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The map label for this gene is nifA [H]

Identifier: 13474878

GI number: 13474878

Start: 4730373

End: 4732124

Strand: Reverse

Name: nifA [H]

Synonym: mll5857

Alternate gene names: 13474878

Gene position: 4732124-4730373 (Counterclockwise)

Preceding gene: 13474879

Following gene: 13474877

Centisome position: 67.26

GC content: 58.28

Gene sequence:

>1752_bases
ATGCTTCCAGATCAGGTCGGTGAAATTGTATCTCGGAACACCCCGCCTGAACCTGCGGTCGAAGCGGCGCGCAATGCGGA
CAGCTTGCTCGGGGGAATCTACGAGATATCGAAGATTCTGACTGCCCCGACCCGGCTGGAGATTACGCTTGCCAATGTCG
CGAATATCCTCTCCTCGTTTGTGCAAATGCGTCATGGCGCAATTGTCGTCCTGGACGCTGAAGGAGAGCCGCAGATTAGC
GCAACTACCGGCGGCGCTGTGCCTCAGTCAGCCATCGGCAGTGTCATACCCCAGGCCGTAATAGACAAAATCGTCGCTAC
TGGAGTGCCGATCGTCATACAAGACACAAGCACGTCGGAGCTGTTTCAGGCCGATCCTCAATCGACCAGCGGCACGATCG
CAACTGCGTTTATCGGTGTCCCGCTAAAGGCTGAGGAAAAGATCTTCGGGACGCTGTCGATCGACCGGGTCAGGAACGGT
ACCACCAAGTTCCGTTACGAGGAGGACTTACGTTTCCTGGCCATGGTCGCCAATCTGGTCGGCCGGACCATCCGCCTGCA
TCGCACTTTGAGCACGGCTGGTCAGCGACTTATCGAGAAGCAACCGAGACCAGAGCAATCGCTCGACGAGGACAGGACCC
ATCCGGCCCGACATCCGCATGTCAAGATCGACGGAATCATCGGAGAAAGTCCCGCACTCAAGCAAGTGCTGGAGATCGTC
TCGGTCGTGGCCAGGACCAATTCCACCGTGCTTCTGCGAGGCGAAAGCGGTACCGGCAAGGAGTTCTTTGCACAGGCCAT
CCATAAGCTTTCGCATCGGAGGGAGAAATCCTTCGTCAAATTGAACTGCGCCGCATTGCCCGAAAGCGTCTTGGAATCGG
AGCTCTTTGGCCACGAGAAGGGCGCCTTCACCGGGGCTATCCTGCAGCGCGCCGGCCGGTTCGAATTGGCGAATGGCGGA
ACCCTGCTGCTTGATGAAATCGGCGAGATTTCGCCTGCCTTTCAAGCCAAGCTGTTGCGCGTCTTGCAGGAAGGAGAACT
TGAGCGAGTGGGCGGCACCAGGACCCTAAAGGTTGACGTGCGGCTCATATGCGCCACCAACAAGGACCTCGAGATGGCTG
TCCGGAATGGAGAGTTCAGGGCCGACCTTTATTACCGCATCAATGTGGTGCCAATAGTCTTGCCTCCCCTCAGAGAGCGG
CCGGGCGATATTCCACGCCTTGCCAAAGCTCTCCTCGATAGATTCAACAAGGAGAACCATCGCGATCTTGCCTTCACGCC
GTCGGCGCTTGACCTGATCTCGCAATGCTATTTCCCTGGCAACGTGCGTGAGTTGGAGAATTGTGTGCGACGGACGGCCA
CACTTGCGCGTTCAATGACAATCACTCCGTCGGATTTCGCCTGCCAGAACAGCCAGTGCCTTTCTTCGCTTCTGTGGAAA
GGAGTCGGCCGTTCGCACGGCGCCTATGCCGTCGACGAGTTCGCACGTGGCAATATGATGCCGGTTGGATCGCCGCTGCC
TGCCATGCGAGTCGGCCCCTCCCAGAATGAGGCATCGCCCGGCGAGACCTGCGACCCCAACCATCCAGCTTGCCCAGCAA
TCAACCAGCGTCTGACGGAACGCGACCGACTGATCGATGCGATGGAGAAAGCCGGCTGGGTTCAGGCCAAGGCGGCTCGT
TTCCTCGGTCTCACGCCGCGGCAGGTCGGCTATGCTCTGCGCCGGCATCATATCGAAGTGAAGAAGTTCTAA

Upstream 100 bases:

>100_bases
TGCCGAAAGGCTGAAAATACTATGTTTCTGGAACTCAAAATACAAAAAAGGGCCTGGGAGGAGCACCTTGGACAACCCTT
GAGGTGTTTGCATGGCTCAC

Downstream 100 bases:

>100_bases
ACGTCCCTGATGACATCAAACGTGGGGGCGCGGTTGCTGCCCGCACCGGGCGTAACTGAGACAAGACGTCACTTTTCGCG
TTGCTTTTGACCGTTGTCGG

Product: nif-specific regulatory protein,nifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MLPDQVGEIVSRNTPPEPAVEAARNADSLLGGIYEISKILTAPTRLEITLANVANILSSFVQMRHGAIVVLDAEGEPQIS
ATTGGAVPQSAIGSVIPQAVIDKIVATGVPIVIQDTSTSELFQADPQSTSGTIATAFIGVPLKAEEKIFGTLSIDRVRNG
TTKFRYEEDLRFLAMVANLVGRTIRLHRTLSTAGQRLIEKQPRPEQSLDEDRTHPARHPHVKIDGIIGESPALKQVLEIV
SVVARTNSTVLLRGESGTGKEFFAQAIHKLSHRREKSFVKLNCAALPESVLESELFGHEKGAFTGAILQRAGRFELANGG
TLLLDEIGEISPAFQAKLLRVLQEGELERVGGTRTLKVDVRLICATNKDLEMAVRNGEFRADLYYRINVVPIVLPPLRER
PGDIPRLAKALLDRFNKENHRDLAFTPSALDLISQCYFPGNVRELENCVRRTATLARSMTITPSDFACQNSQCLSSLLWK
GVGRSHGAYAVDEFARGNMMPVGSPLPAMRVGPSQNEASPGETCDPNHPACPAINQRLTERDRLIDAMEKAGWVQAKAAR
FLGLTPRQVGYALRRHHIEVKKF

Sequences:

>Translated_583_residues
MLPDQVGEIVSRNTPPEPAVEAARNADSLLGGIYEISKILTAPTRLEITLANVANILSSFVQMRHGAIVVLDAEGEPQIS
ATTGGAVPQSAIGSVIPQAVIDKIVATGVPIVIQDTSTSELFQADPQSTSGTIATAFIGVPLKAEEKIFGTLSIDRVRNG
TTKFRYEEDLRFLAMVANLVGRTIRLHRTLSTAGQRLIEKQPRPEQSLDEDRTHPARHPHVKIDGIIGESPALKQVLEIV
SVVARTNSTVLLRGESGTGKEFFAQAIHKLSHRREKSFVKLNCAALPESVLESELFGHEKGAFTGAILQRAGRFELANGG
TLLLDEIGEISPAFQAKLLRVLQEGELERVGGTRTLKVDVRLICATNKDLEMAVRNGEFRADLYYRINVVPIVLPPLRER
PGDIPRLAKALLDRFNKENHRDLAFTPSALDLISQCYFPGNVRELENCVRRTATLARSMTITPSDFACQNSQCLSSLLWK
GVGRSHGAYAVDEFARGNMMPVGSPLPAMRVGPSQNEASPGETCDPNHPACPAINQRLTERDRLIDAMEKAGWVQAKAAR
FLGLTPRQVGYALRRHHIEVKKF
>Mature_583_residues
MLPDQVGEIVSRNTPPEPAVEAARNADSLLGGIYEISKILTAPTRLEITLANVANILSSFVQMRHGAIVVLDAEGEPQIS
ATTGGAVPQSAIGSVIPQAVIDKIVATGVPIVIQDTSTSELFQADPQSTSGTIATAFIGVPLKAEEKIFGTLSIDRVRNG
TTKFRYEEDLRFLAMVANLVGRTIRLHRTLSTAGQRLIEKQPRPEQSLDEDRTHPARHPHVKIDGIIGESPALKQVLEIV
SVVARTNSTVLLRGESGTGKEFFAQAIHKLSHRREKSFVKLNCAALPESVLESELFGHEKGAFTGAILQRAGRFELANGG
TLLLDEIGEISPAFQAKLLRVLQEGELERVGGTRTLKVDVRLICATNKDLEMAVRNGEFRADLYYRINVVPIVLPPLRER
PGDIPRLAKALLDRFNKENHRDLAFTPSALDLISQCYFPGNVRELENCVRRTATLARSMTITPSDFACQNSQCLSSLLWK
GVGRSHGAYAVDEFARGNMMPVGSPLPAMRVGPSQNEASPGETCDPNHPACPAINQRLTERDRLIDAMEKAGWVQAKAAR
FLGLTPRQVGYALRRHHIEVKKF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=241, Percent_Identity=54.356846473029, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1790437, Length=258, Percent_Identity=53.1007751937985, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1788550, Length=260, Percent_Identity=50.3846153846154, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1789087, Length=233, Percent_Identity=55.3648068669528, Blast_Score=255, Evalue=7e-69,
Organism=Escherichia coli, GI87082152, Length=239, Percent_Identity=51.8828451882845, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1790299, Length=252, Percent_Identity=47.2222222222222, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1788905, Length=241, Percent_Identity=48.5477178423236, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI87081872, Length=358, Percent_Identity=39.1061452513966, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1789233, Length=233, Percent_Identity=45.4935622317597, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1786524, Length=282, Percent_Identity=41.8439716312057, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1787583, Length=241, Percent_Identity=43.9834024896266, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789828, Length=253, Percent_Identity=41.1067193675889, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081858, Length=238, Percent_Identity=32.7731092436975, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 63747; Mature: 63747

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDQVGEIVSRNTPPEPAVEAARNADSLLGGIYEISKILTAPTRLEITLANVANILSSF
CCCHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
VQMRHGAIVVLDAEGEPQISATTGGAVPQSAIGSVIPQAVIDKIVATGVPIVIQDTSTSE
HHHHCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
LFQADPQSTSGTIATAFIGVPLKAEEKIFGTLSIDRVRNGTTKFRYEEDLRFLAMVANLV
HHHCCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHCCCCCCEEEHHHHHHHHHHHHHH
GRTIRLHRTLSTAGQRLIEKQPRPEQSLDEDRTHPARHPHVKIDGIIGESPALKQVLEIV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEEEECEECCCHHHHHHHHHH
SVVARTNSTVLLRGESGTGKEFFAQAIHKLSHRREKSFVKLNCAALPESVLESELFGHEK
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEHHHCCHHHHHHHHHCCCC
GAFTGAILQRAGRFELANGGTLLLDEIGEISPAFQAKLLRVLQEGELERVGGTRTLKVDV
CCHHHHHHHHCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEE
RLICATNKDLEMAVRNGEFRADLYYRINVVPIVLPPLRERPGDIPRLAKALLDRFNKENH
EEEEECCCCCEEHCCCCCEEEEEEEEEEEEEEECCCHHCCCCCHHHHHHHHHHHHCCCCC
RDLAFTPSALDLISQCYFPGNVRELENCVRRTATLARSMTITPSDFACQNSQCLSSLLWK
CCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHH
GVGRSHGAYAVDEFARGNMMPVGSPLPAMRVGPSQNEASPGETCDPNHPACPAINQRLTE
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RDRLIDAMEKAGWVQAKAARFLGLTPRQVGYALRRHHIEVKKF
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCEECCC
>Mature Secondary Structure
MLPDQVGEIVSRNTPPEPAVEAARNADSLLGGIYEISKILTAPTRLEITLANVANILSSF
CCCHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHH
VQMRHGAIVVLDAEGEPQISATTGGAVPQSAIGSVIPQAVIDKIVATGVPIVIQDTSTSE
HHHHCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHH
LFQADPQSTSGTIATAFIGVPLKAEEKIFGTLSIDRVRNGTTKFRYEEDLRFLAMVANLV
HHHCCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHCCCCCCEEEHHHHHHHHHHHHHH
GRTIRLHRTLSTAGQRLIEKQPRPEQSLDEDRTHPARHPHVKIDGIIGESPALKQVLEIV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEEEECEECCCHHHHHHHHHH
SVVARTNSTVLLRGESGTGKEFFAQAIHKLSHRREKSFVKLNCAALPESVLESELFGHEK
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEHHHCCHHHHHHHHHCCCC
GAFTGAILQRAGRFELANGGTLLLDEIGEISPAFQAKLLRVLQEGELERVGGTRTLKVDV
CCHHHHHHHHCCCEEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEE
RLICATNKDLEMAVRNGEFRADLYYRINVVPIVLPPLRERPGDIPRLAKALLDRFNKENH
EEEEECCCCCEEHCCCCCEEEEEEEEEEEEEEECCCHHCCCCCHHHHHHHHHHHHCCCCC
RDLAFTPSALDLISQCYFPGNVRELENCVRRTATLARSMTITPSDFACQNSQCLSSLLWK
CCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHH
GVGRSHGAYAVDEFARGNMMPVGSPLPAMRVGPSQNEASPGETCDPNHPACPAINQRLTE
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RDRLIDAMEKAGWVQAKAARFLGLTPRQVGYALRRHHIEVKKF
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12801410 [H]