Click here to switch to the map view.

The map label for this gene is bioB [H]

Identifier: 13474856

GI number: 13474856

Start: 4703847

End: 4704842

Strand: Reverse

Name: bioB [H]

Synonym: mll5831

Alternate gene names: 13474856

Gene position: 4704842-4703847 (Counterclockwise)

Preceding gene: 13474857

Following gene: 13474855

Centisome position: 66.87

GC content: 62.25

Gene sequence:

>996_bases
ATGAACGAACTCAGCCCAGACCTGACCTCGGATCCAATCGGTTGGGCGCTTCCCCCTTGGACGCGAGCGGAAGCCCAAGC
GCTCCACGACGCGCCATTCAACGACCTTTTGTTTCAGGCGCAGACCGTTCACCGGCAGAACTTCGATCCCAACAAGGTCC
AGCTGAGCCGGCTTCTCAGCATCAAGACCGGCGGATGCCCGGAGGATTGCGGCTATTGCAGCCAATCGGCACATCACGAA
AGCGGGTTGAAGGCGTCAAAGCTGATGGAGGTCCGGCGCGTCATCGCCGAGGCGACCAAGGCACGCGATGCCGGCGCCAC
CCGCTATTGCATGGGCGCGGCCTGGCGAAACCCGAAGGCGCGCGACATGGACGCGGTGGTGGCGATGGTCGAGGGTGTCA
AGGCACTCGGCATGGAGACCTGCATGACGCTCGGCATGCTCGATCTTGAGCAGGCCGCTCGTCTGAAACAGGCCGGTCTC
GACTACTACAACCACAACATCGACACCTCCGAGCGCTATTACTCAGAGATCATCTCGACCCGCACGTTTGCCGACCGGCT
GGATACGCTCGCCAATGTCCGCGACAGCGGCATTAAGGTCTGTTGCGGCGGCATTGTCGGCATGGGCGAAGAGCCGGTGG
ACCGGATCGACATGCTGGTGACGCTGGCCAACCTGCCGGAGCATCCCGAAAGCGTTCCGATCAACATGCTGATCCCGATC
GAGGGCACCCCGCTGGCCGAGGCCAAACCCATCGAGCCGATCGAATTCGTGCGCGTGATCGCGCTGGCCCGCATCATGAT
GCCGAAGTCGCACGTGCGTCTTTCCGCCGGCCGCACCGCCATGACCGATGAAATGCAGGCGCTGTGCTTTTTTGCCGGCG
CCAACTCGATCTTCGTCGGCGACACACTGCTGACGGCGGACAATCCCGGCGAAGACAAGGATACTCTGCTGTTCCAGCGT
CTCGGCATCGAGCCGATGGAAATCGGCACGCAATGA

Upstream 100 bases:

>100_bases
CGCGTTTCGATAGAAGACTTCCCGCTGGCATGCGCCTGGTCTTCAGCATCCACTGCAACGCTCCAGGTCAAGGACGGAAG
ATTATGGAGAACAAGATTTT

Downstream 100 bases:

>100_bases
ACGAGGCACTTCTTGTCCGGTATGACGCGTCCTTGCGCGGACTGGCGCGCAAGGACCGGCTGCGCACACTTGCACCGCGC
GCCGGGCTCGACTTCTCGTC

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNELSPDLTSDPIGWALPPWTRAEAQALHDAPFNDLLFQAQTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHE
SGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGL
DYYNHNIDTSERYYSEIISTRTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPI
EGTPLAEAKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDKDTLLFQR
LGIEPMEIGTQ

Sequences:

>Translated_331_residues
MNELSPDLTSDPIGWALPPWTRAEAQALHDAPFNDLLFQAQTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHE
SGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGL
DYYNHNIDTSERYYSEIISTRTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPI
EGTPLAEAKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDKDTLLFQR
LGIEPMEIGTQ
>Mature_331_residues
MNELSPDLTSDPIGWALPPWTRAEAQALHDAPFNDLLFQAQTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHE
SGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKARDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGL
DYYNHNIDTSERYYSEIISTRTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPI
EGTPLAEAKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDKDTLLFQR
LGIEPMEIGTQ

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=310, Percent_Identity=60, Blast_Score=403, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=49.0506329113924, Blast_Score=329, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36323; Mature: 36323

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELSPDLTSDPIGWALPPWTRAEAQALHDAPFNDLLFQAQTVHRQNFDPNKVQLSRLLS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IKTGGCPEDCGYCSQSAHHESGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKA
HCCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
RDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNIDTSERYYSEIIST
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHH
RTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
EGTPLAEAKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHCCCCEEEEC
DTLLTADNPGEDKDTLLFQRLGIEPMEIGTQ
CEEEECCCCCCCHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MNELSPDLTSDPIGWALPPWTRAEAQALHDAPFNDLLFQAQTVHRQNFDPNKVQLSRLLS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IKTGGCPEDCGYCSQSAHHESGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKA
HCCCCCCHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
RDMDAVVAMVEGVKALGMETCMTLGMLDLEQAARLKQAGLDYYNHNIDTSERYYSEIIST
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHH
RTFADRLDTLANVRDSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPESVPINMLIPI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
EGTPLAEAKPIEPIEFVRVIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHCCCCEEEEC
DTLLTADNPGEDKDTLLFQRLGIEPMEIGTQ
CEEEECCCCCCCHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]