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The map label for this gene is tktB [C]

Identifier: 13474797

GI number: 13474797

Start: 4646108

End: 4646947

Strand: Reverse

Name: tktB [C]

Synonym: mll5765

Alternate gene names: 13474797

Gene position: 4646947-4646108 (Counterclockwise)

Preceding gene: 13474798

Following gene: 13474796

Centisome position: 66.04

GC content: 63.81

Gene sequence:

>840_bases
ATGCCACGCGCGGGCAGCAATGTCAGCCTTGCCGAACGCGCCTATCGCATCCGTCGACATGCCGTGCGCATGGGCGAAGT
CCAGGGCCAGGGCTATGTCGGGCAGGCGCTCGGCGTCGCCGATGTGCTTGCGGTCGCCTATTTCCATGCACTTCGCTACC
GGCCGGACGACACGCTGTGGGAGGGACGCGATCGCTTCCTCCTATCCATCGGACACTATGCGATCGCACTCTACGCGGCA
TTGATCGAAGCAGGCGTGCTGCCGGAGGAAGAGCTCGAGACCTACGGTACCGACGACAGCCGCATGCCGATGTCCGGCAT
GGCGGCGTACACACCTGGCATGGAGATCACCGGCGGCTCGCTTGGTCACGGTCTTGGTATTGCCGTCGGCATGTGCCTGG
GACTGAAACGCAAGCAGAGCGATTGTTTCGTCTACAACCTCTTGTCCGACGGTGAGCTTGGCGAGGGTTCGACCTGGGAA
GCAGCGATGTCTGCCGCCCATCACAAGCTCGACAACCTCATCGCCATCGTCGACTTCAACAACCTGCAGGCCGACGGCCC
CTCCACAGCCATGCTGTCTTCGGAGCCGGTGACCGACAAGTTCGAGGCCTTCGGCTGGCACGCGCAGCGCGTTGACGGTA
ACGACATCGAGGCCCTCCGCGCTGCGTTCGACAACGCCCGGGGCCTCAAGGATCCGCGCCCGCGGATCATCGTCTGCGAC
ACCCGCATGGCGAAGGGTGTGCCGTTCCTGGAGGCTCGCGACATCACGCATTTCATCCGCGTCGAGCCCTATGAGTGGGC
TCTGGCGCTCGAGGCTCTGGAAAAGGGACGTCCGCAGTGA

Upstream 100 bases:

>100_bases
CTCTTCCTCGCCTCCGATCTGGCCAGCTACATCACCGGTGCCGTGATCGACGTCAATGGGGGGATGCTGATCCATGGCTG
ACGGGACAACGGAATTCGAG

Downstream 100 bases:

>100_bases
GGCAGTCAAAATTCGCGCCGCGCCACGCCGTCAACGCGGGTGAGCGCAAGACCACCTCGGCGATGATCGCCTCGATCGCG
CCCAATGGCGTTCCGACGCG

Product: transketolase

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MPRAGSNVSLAERAYRIRRHAVRMGEVQGQGYVGQALGVADVLAVAYFHALRYRPDDTLWEGRDRFLLSIGHYAIALYAA
LIEAGVLPEEELETYGTDDSRMPMSGMAAYTPGMEITGGSLGHGLGIAVGMCLGLKRKQSDCFVYNLLSDGELGEGSTWE
AAMSAAHHKLDNLIAIVDFNNLQADGPSTAMLSSEPVTDKFEAFGWHAQRVDGNDIEALRAAFDNARGLKDPRPRIIVCD
TRMAKGVPFLEARDITHFIRVEPYEWALALEALEKGRPQ

Sequences:

>Translated_279_residues
MPRAGSNVSLAERAYRIRRHAVRMGEVQGQGYVGQALGVADVLAVAYFHALRYRPDDTLWEGRDRFLLSIGHYAIALYAA
LIEAGVLPEEELETYGTDDSRMPMSGMAAYTPGMEITGGSLGHGLGIAVGMCLGLKRKQSDCFVYNLLSDGELGEGSTWE
AAMSAAHHKLDNLIAIVDFNNLQADGPSTAMLSSEPVTDKFEAFGWHAQRVDGNDIEALRAAFDNARGLKDPRPRIIVCD
TRMAKGVPFLEARDITHFIRVEPYEWALALEALEKGRPQ
>Mature_278_residues
PRAGSNVSLAERAYRIRRHAVRMGEVQGQGYVGQALGVADVLAVAYFHALRYRPDDTLWEGRDRFLLSIGHYAIALYAAL
IEAGVLPEEELETYGTDDSRMPMSGMAAYTPGMEITGGSLGHGLGIAVGMCLGLKRKQSDCFVYNLLSDGELGEGSTWEA
AMSAAHHKLDNLIAIVDFNNLQADGPSTAMLSSEPVTDKFEAFGWHAQRVDGNDIEALRAAFDNARGLKDPRPRIIVCDT
RMAKGVPFLEARDITHFIRVEPYEWALALEALEKGRPQ

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=274, Percent_Identity=36.4963503649635, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI205277463, Length=253, Percent_Identity=37.1541501976285, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4507521, Length=253, Percent_Identity=37.1541501976285, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI225637459, Length=251, Percent_Identity=29.4820717131474, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI225637461, Length=251, Percent_Identity=29.0836653386454, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI225637463, Length=167, Percent_Identity=37.125748502994, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788808, Length=267, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI48994911, Length=279, Percent_Identity=32.9749103942652, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=34.5238095238095, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319593, Length=243, Percent_Identity=33.7448559670782, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325331, Length=244, Percent_Identity=35.655737704918, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45551847, Length=260, Percent_Identity=32.6923076923077, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550715, Length=260, Percent_Identity=32.6923076923077, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24666278, Length=246, Percent_Identity=31.7073170731707, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645119, Length=219, Percent_Identity=35.1598173515982, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30584; Mature: 30453

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRAGSNVSLAERAYRIRRHAVRMGEVQGQGYVGQALGVADVLAVAYFHALRYRPDDTLW
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGRDRFLLSIGHYAIALYAALIEAGVLPEEELETYGTDDSRMPMSGMAAYTPGMEITGGS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
LGHGLGIAVGMCLGLKRKQSDCFVYNLLSDGELGEGSTWEAAMSAAHHKLDNLIAIVDFN
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECC
NLQADGPSTAMLSSEPVTDKFEAFGWHAQRVDGNDIEALRAAFDNARGLKDPRPRIIVCD
CCCCCCCCCEEECCCCCCHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
TRMAKGVPFLEARDITHFIRVEPYEWALALEALEKGRPQ
CHHHCCCCCHHHHCCEEEEEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRAGSNVSLAERAYRIRRHAVRMGEVQGQGYVGQALGVADVLAVAYFHALRYRPDDTLW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGRDRFLLSIGHYAIALYAALIEAGVLPEEELETYGTDDSRMPMSGMAAYTPGMEITGGS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
LGHGLGIAVGMCLGLKRKQSDCFVYNLLSDGELGEGSTWEAAMSAAHHKLDNLIAIVDFN
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECC
NLQADGPSTAMLSSEPVTDKFEAFGWHAQRVDGNDIEALRAAFDNARGLKDPRPRIIVCD
CCCCCCCCCEEECCCCCCHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEC
TRMAKGVPFLEARDITHFIRVEPYEWALALEALEKGRPQ
CHHHCCCCCHHHHCCEEEEEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]