Click here to switch to the map view.

The map label for this gene is cusS [H]

Identifier: 13474721

GI number: 13474721

Start: 4561875

End: 4563308

Strand: Reverse

Name: cusS [H]

Synonym: mll5673

Alternate gene names: 13474721

Gene position: 4563308-4561875 (Counterclockwise)

Preceding gene: 13474722

Following gene: 13474720

Centisome position: 64.86

GC content: 65.41

Gene sequence:

>1434_bases
GTGAAGAAGAAGGTCCGCACAGGCTCGCTGCAATGGATCCTGGTCCGGCGGCTGATCCTGCTGCAGGCGGCGACGCTGCT
CATCTTCATCGTGCTTTGCGCGGCAGCCCTTTGGATCGCCAATCCGCGCCTGCTGATCGACAATGAGGCCGCCGTCGCGG
CCGTCAAGGATGCGGTCGACCGGGACAAGGACGGCAGGCTGATCGTGCGTGAAACGGAAGAACTCACCGCGTTTCGCGAG
AGCTTCCCCAATGTCTGGTACATTGTTCGCGATGGCAGGGGCGAGAGCGTCCATGCCGGCAACATACCCGACATCTACAC
CAAGAGCTTCGGCGAGCTGCTTGACGGGGCCGATCACGCCACGATCGGATTCTCCGACAAGGACATGCGGCCGGAAGCCT
ATATCGAAAACGCCTCGACCAAGGCCGGCACGGTGCAGATCATCGCCGCGACACAGTCGTCGCGCCAACAGACAGATGGC
CTCGAGATCAACATCTCGGCCACCGTCGAAGTCGCCCGCAACCCGGACGGCAGCAGGAACTGGGAGCGCGCCCTGCCAGC
GCTCGCGCTCGTCATCGCGATCCTGCTTTTGCCCATCATTCTCGTCATGGGCACGACGACGCTGGTGACGACGCCAGCCG
TGGTGCGCCGCTCCTTCGCCGGCCTTGTCGAAACGGTCCGCCAGGCGGCGCGTATCGACATCGGCACCCGCGCCATGCAA
CTGCCGGTCAAGCAGGTGCCGCAGGAAATCGCGCCGCTCGTGCATGCCTTCAACGAGGCGCTTGCCCGCCTCGCCCAGGG
CTATGACCGCCACAACCGCTTCCTTACCGACGCGGCGCATGAACTGCGCACGCCGATCGCCATCCTTCGTACGCGCGCCG
AACTCTTGACGCAGGAGCCGCAGAGCGCGCGGCTGCTGCAAGACATCGAACGCCTGTCGCATCTTGCCCAGCAACTGCTC
GACCATCAGTTGCTCGACCGCCCGTCGGACCAGCGCCAGCTCGTCGATCTCGGCGATCTCGTCAGCCGGGTGGCGGCCGA
CTTCGCACCGCTCGCCATCGAGGCTGGCTATGACCTTGCCTTCGAGCCGTCGCCAAACAAGGCCCGAGTAGAGATCAATG
TCCTGCAGATCGAGCGGGCGCTCGCCAACCTCGTGCGCAACGCGATCGAGCACGGCGGCGGCAGCGGCACGATCACGATC
GCCATCGACGGGACCGGTGGCATCGAGGTACGCGACGAAGGGCCGGGCATTCCCGCGGAAGAGCGCGAGAACGTCTTCGA
GCCCTTCTACCGCTTGCAGCCGCAGTCGCGCGGCGCGGGGCTGGGACTGAATCTCGCCCGGCAGATCGCGTTGCTGCATG
ACGGCACCATCCGGATCCTGACGGGTTCATGGCGCGGTGCCCGCATCCGCATGGAGTTGCCGGTGCGTTCTTGA

Upstream 100 bases:

>100_bases
ATGCGCATATTTCGCGGCTGCGTCGCAAGCTCGACGATGTCGCCGCCGGCATCGAGATCCGGGCGATCCGCAACCTCGGC
TATCTCCTGCGGGCGGTCTC

Downstream 100 bases:

>100_bases
CCTGCCGAGCCCATGGCGTCGGCCGGCCGAAGCCCATGTCGATCCCCGGCTTATCTGTTTTCTAACCAATTGCCGCTATT
GCCATTGCAAGAGCGGGAGC

Product: two-component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKKKVRTGSLQWILVRRLILLQAATLLIFIVLCAAALWIANPRLLIDNEAAVAAVKDAVDRDKDGRLIVRETEELTAFRE
SFPNVWYIVRDGRGESVHAGNIPDIYTKSFGELLDGADHATIGFSDKDMRPEAYIENASTKAGTVQIIAATQSSRQQTDG
LEINISATVEVARNPDGSRNWERALPALALVIAILLLPIILVMGTTTLVTTPAVVRRSFAGLVETVRQAARIDIGTRAMQ
LPVKQVPQEIAPLVHAFNEALARLAQGYDRHNRFLTDAAHELRTPIAILRTRAELLTQEPQSARLLQDIERLSHLAQQLL
DHQLLDRPSDQRQLVDLGDLVSRVAADFAPLAIEAGYDLAFEPSPNKARVEINVLQIERALANLVRNAIEHGGGSGTITI
AIDGTGGIEVRDEGPGIPAEERENVFEPFYRLQPQSRGAGLGLNLARQIALLHDGTIRILTGSWRGARIRMELPVRS

Sequences:

>Translated_477_residues
MKKKVRTGSLQWILVRRLILLQAATLLIFIVLCAAALWIANPRLLIDNEAAVAAVKDAVDRDKDGRLIVRETEELTAFRE
SFPNVWYIVRDGRGESVHAGNIPDIYTKSFGELLDGADHATIGFSDKDMRPEAYIENASTKAGTVQIIAATQSSRQQTDG
LEINISATVEVARNPDGSRNWERALPALALVIAILLLPIILVMGTTTLVTTPAVVRRSFAGLVETVRQAARIDIGTRAMQ
LPVKQVPQEIAPLVHAFNEALARLAQGYDRHNRFLTDAAHELRTPIAILRTRAELLTQEPQSARLLQDIERLSHLAQQLL
DHQLLDRPSDQRQLVDLGDLVSRVAADFAPLAIEAGYDLAFEPSPNKARVEINVLQIERALANLVRNAIEHGGGSGTITI
AIDGTGGIEVRDEGPGIPAEERENVFEPFYRLQPQSRGAGLGLNLARQIALLHDGTIRILTGSWRGARIRMELPVRS
>Mature_477_residues
MKKKVRTGSLQWILVRRLILLQAATLLIFIVLCAAALWIANPRLLIDNEAAVAAVKDAVDRDKDGRLIVRETEELTAFRE
SFPNVWYIVRDGRGESVHAGNIPDIYTKSFGELLDGADHATIGFSDKDMRPEAYIENASTKAGTVQIIAATQSSRQQTDG
LEINISATVEVARNPDGSRNWERALPALALVIAILLLPIILVMGTTTLVTTPAVVRRSFAGLVETVRQAARIDIGTRAMQ
LPVKQVPQEIAPLVHAFNEALARLAQGYDRHNRFLTDAAHELRTPIAILRTRAELLTQEPQSARLLQDIERLSHLAQQLL
DHQLLDRPSDQRQLVDLGDLVSRVAADFAPLAIEAGYDLAFEPSPNKARVEINVLQIERALANLVRNAIEHGGGSGTITI
AIDGTGGIEVRDEGPGIPAEERENVFEPFYRLQPQSRGAGLGLNLARQIALLHDGTIRILTGSWRGARIRMELPVRS

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=303, Percent_Identity=29.3729372937294, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789808, Length=256, Percent_Identity=27.734375, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787894, Length=229, Percent_Identity=31.4410480349345, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790346, Length=239, Percent_Identity=28.8702928870293, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789403, Length=312, Percent_Identity=30.4487179487179, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788279, Length=322, Percent_Identity=25.776397515528, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=272, Percent_Identity=27.5735294117647, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=26.7657992565056, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=29.3617021276596, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788549, Length=218, Percent_Identity=27.9816513761468, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787374, Length=354, Percent_Identity=24.8587570621469, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=26.3598326359833, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=26.3636363636364, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790436, Length=209, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=225, Percent_Identity=28.8888888888889, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52430; Mature: 52430

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN ; PS00664 VINCULIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVRTGSLQWILVRRLILLQAATLLIFIVLCAAALWIANPRLLIDNEAAVAAVKDAVD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHC
RDKDGRLIVRETEELTAFRESFPNVWYIVRDGRGESVHAGNIPDIYTKSFGELLDGADHA
CCCCCCEEEECHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCC
TIGFSDKDMRPEAYIENASTKAGTVQIIAATQSSRQQTDGLEINISATVEVARNPDGSRN
EECCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCC
WERALPALALVIAILLLPIILVMGTTTLVTTPAVVRRSFAGLVETVRQAARIDIGTRAMQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LPVKQVPQEIAPLVHAFNEALARLAQGYDRHNRFLTDAAHELRTPIAILRTRAELLTQEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
QSARLLQDIERLSHLAQQLLDHQLLDRPSDQRQLVDLGDLVSRVAADFAPLAIEAGYDLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
FEPSPNKARVEINVLQIERALANLVRNAIEHGGGSGTITIAIDGTGGIEVRDEGPGIPAE
ECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCCHH
ERENVFEPFYRLQPQSRGAGLGLNLARQIALLHDGTIRILTGSWRGARIRMELPVRS
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEECCEEEEEECCCCCCEEEEECCCCC
>Mature Secondary Structure
MKKKVRTGSLQWILVRRLILLQAATLLIFIVLCAAALWIANPRLLIDNEAAVAAVKDAVD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHC
RDKDGRLIVRETEELTAFRESFPNVWYIVRDGRGESVHAGNIPDIYTKSFGELLDGADHA
CCCCCCEEEECHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCC
TIGFSDKDMRPEAYIENASTKAGTVQIIAATQSSRQQTDGLEINISATVEVARNPDGSRN
EECCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCC
WERALPALALVIAILLLPIILVMGTTTLVTTPAVVRRSFAGLVETVRQAARIDIGTRAMQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LPVKQVPQEIAPLVHAFNEALARLAQGYDRHNRFLTDAAHELRTPIAILRTRAELLTQEP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
QSARLLQDIERLSHLAQQLLDHQLLDRPSDQRQLVDLGDLVSRVAADFAPLAIEAGYDLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
FEPSPNKARVEINVLQIERALANLVRNAIEHGGGSGTITIAIDGTGGIEVRDEGPGIPAE
ECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCCHH
ERENVFEPFYRLQPQSRGAGLGLNLARQIALLHDGTIRILTGSWRGARIRMELPVRS
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEECCEEEEEECCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]