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The map label for this gene is pucE [H]

Identifier: 13474281

GI number: 13474281

Start: 4070886

End: 4072367

Strand: Direct

Name: pucE [H]

Synonym: mlr5134

Alternate gene names: 13474281

Gene position: 4070886-4072367 (Clockwise)

Preceding gene: 13474280

Following gene: 13474282

Centisome position: 57.86

GC content: 64.44

Gene sequence:

>1482_bases
ATGGCCAAGGTCAACATTCGTAACGAGATCCGGTTCATCCTCAATGGCAGGGATGTCGTGCTGTCCTCGGTGGCGCCGGA
CGCGACCCTGCTCGACTGGCTGCGGCTCGACCGTTCGCTACGCGGCACCAAGGAAGGCTGCGCCGAAGGCGATTGCGGTG
CCTGCACCGTTCTGGTCGGCCGACTTTCGGCCGGCGGCCTGATCTATGAAAGCGTCAATGCCTGTATCCGCTTCCTCGGT
TCGCTGGACGGCACGCATGTCGTGACGGTGGAACATTTGCGTGGTCAGCCCGGCCAGTTGCATCCGGTCCAGCAGGCGAT
GGTCGATTTCCATGGCTCGCAATGCGGCTTCTGCACGCCGGGCTTCGTCATGTCGCTCTATGGTCTGTGGATGAAGTCGC
CGGATCCCTCGAACGCGGCGATTGAAAAGGCCTTGCAAGGCAATCTCTGCCGCTGCACCGGCTATGAGGCGATCATGCGC
GCCGCGCACGCCATCTCCAGCTACGGCAAGGCGGCGAAGGATCCTTTGGCTGTCGAGCGCAAGGCGATCACGGCGAGGCT
GGAAGCCTTGCGGGACGGTGCGCGGGTCGAGATCGGTTCGGCCAAGGCGCGGCTCATCGTGCCGGCCAATGTCGACGATT
TCGCCACCGTCCTGGACCAGGAACCCGGCGCCACCATCGTTGCCGGCTCGACCGATGTCGGCTTGTGGGTGACCAAGCAC
ATGCGCGACATCTCTCCGGCGGTCTTCATAGGCAATCTCGACGGGCTGTGCACCGTCTCCGAAGACAAGGGCGTCATATC
GATCGGGGCCGGCGTCACCTACACCGAAGCCTTTTCGATGCTCGCCAAACGCATCCCGGCGTTGGGGCCGCTGTTTGACC
GCATCGGCGGCGAACAGGTGCGCAACATGGGCACCATCGGCGGCAACATCGCCAACGGCTCGCCGATCGGCGACACGCCG
CCGCCCTTGATCGCGCTCGGCGCGCGCCTGACGCTGCGCAAGGGCCAGAAGCGGCGCACGATACCGCTGGAAGCCTTCTT
CATCGCCTATGGCAGGCAGGACCGGCAACCCGGCGAATTCGTCGAGGCCGTGCACGTGCCCGTGCCGGCCAAGGGAGAGA
AATTTGCCGTCTACAAAATCACCAAGCGGCGCGACGAGGATATCACGGCGGCCCTCGGCGCCTTCTTGCTGACGCTGGCC
AAGGACGGCACGGTCGCCGATGTGCGCATCGCCTATGGCGGCATGGCGGCGACGCCGAAGCGGGCGTCTTTTGTCGAAAA
GGCGCTGCTCGGCAAGCCGTGGACCGAAGAGACGGTCGAGACGGCGATGGCGCAATACGCTGGGGATTTCACCCCGCTCA
CCGACATGCGGGCGTCAGCCGAATACCGGGCTCTGGCAGCCAAGAATTTGTTGTTGCGTTTCTTCGTCGAAACCACCGGC
ACCAGGGCACCCTTCCAGGTGTCCCGCAACGAGGCGGCATGA

Upstream 100 bases:

>100_bases
CAGCGTGCCGATCCGTTTCGGCATGGCGGAGGCGGTGCACTATCACGTGCCATTGCTGATCTCGCCCTATGGCTATTCGA
CCTACAGGGGGAGCTGAGGC

Downstream 100 bases:

>100_bases
TGAACAAGCACGCCACATCAAACCTCAAAGCCGAGAAGATCGTCGGCGGCGTCGCCACCGATCAGCGGCATGATTCCGCC
CACAAGCATGTCAGCGGCAC

Product: xanthine dehydrogenase, xdhA

Products: NA

Alternate protein names: XDHase subunit E [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAKVNIRNEIRFILNGRDVVLSSVAPDATLLDWLRLDRSLRGTKEGCAEGDCGACTVLVGRLSAGGLIYESVNACIRFLG
SLDGTHVVTVEHLRGQPGQLHPVQQAMVDFHGSQCGFCTPGFVMSLYGLWMKSPDPSNAAIEKALQGNLCRCTGYEAIMR
AAHAISSYGKAAKDPLAVERKAITARLEALRDGARVEIGSAKARLIVPANVDDFATVLDQEPGATIVAGSTDVGLWVTKH
MRDISPAVFIGNLDGLCTVSEDKGVISIGAGVTYTEAFSMLAKRIPALGPLFDRIGGEQVRNMGTIGGNIANGSPIGDTP
PPLIALGARLTLRKGQKRRTIPLEAFFIAYGRQDRQPGEFVEAVHVPVPAKGEKFAVYKITKRRDEDITAALGAFLLTLA
KDGTVADVRIAYGGMAATPKRASFVEKALLGKPWTEETVETAMAQYAGDFTPLTDMRASAEYRALAAKNLLLRFFVETTG
TRAPFQVSRNEAA

Sequences:

>Translated_493_residues
MAKVNIRNEIRFILNGRDVVLSSVAPDATLLDWLRLDRSLRGTKEGCAEGDCGACTVLVGRLSAGGLIYESVNACIRFLG
SLDGTHVVTVEHLRGQPGQLHPVQQAMVDFHGSQCGFCTPGFVMSLYGLWMKSPDPSNAAIEKALQGNLCRCTGYEAIMR
AAHAISSYGKAAKDPLAVERKAITARLEALRDGARVEIGSAKARLIVPANVDDFATVLDQEPGATIVAGSTDVGLWVTKH
MRDISPAVFIGNLDGLCTVSEDKGVISIGAGVTYTEAFSMLAKRIPALGPLFDRIGGEQVRNMGTIGGNIANGSPIGDTP
PPLIALGARLTLRKGQKRRTIPLEAFFIAYGRQDRQPGEFVEAVHVPVPAKGEKFAVYKITKRRDEDITAALGAFLLTLA
KDGTVADVRIAYGGMAATPKRASFVEKALLGKPWTEETVETAMAQYAGDFTPLTDMRASAEYRALAAKNLLLRFFVETTG
TRAPFQVSRNEAA
>Mature_492_residues
AKVNIRNEIRFILNGRDVVLSSVAPDATLLDWLRLDRSLRGTKEGCAEGDCGACTVLVGRLSAGGLIYESVNACIRFLGS
LDGTHVVTVEHLRGQPGQLHPVQQAMVDFHGSQCGFCTPGFVMSLYGLWMKSPDPSNAAIEKALQGNLCRCTGYEAIMRA
AHAISSYGKAAKDPLAVERKAITARLEALRDGARVEIGSAKARLIVPANVDDFATVLDQEPGATIVAGSTDVGLWVTKHM
RDISPAVFIGNLDGLCTVSEDKGVISIGAGVTYTEAFSMLAKRIPALGPLFDRIGGEQVRNMGTIGGNIANGSPIGDTPP
PLIALGARLTLRKGQKRRTIPLEAFFIAYGRQDRQPGEFVEAVHVPVPAKGEKFAVYKITKRRDEDITAALGAFLLTLAK
DGTVADVRIAYGGMAATPKRASFVEKALLGKPWTEETVETAMAQYAGDFTPLTDMRASAEYRALAAKNLLLRFFVETTGT
RAPFQVSRNEAA

Specific function: Oxidizes hypoxanthine and xanthine to uric acid [H]

COG id: COG4630

COG function: function code F; Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI91823271, Length=520, Percent_Identity=30.9615384615385, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI71773480, Length=531, Percent_Identity=27.3069679849341, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1789231, Length=260, Percent_Identity=29.6153846153846, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786480, Length=164, Percent_Identity=35.9756097560976, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789232, Length=153, Percent_Identity=35.2941176470588, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32566215, Length=160, Percent_Identity=45.625, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17540638, Length=156, Percent_Identity=44.2307692307692, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17539860, Length=158, Percent_Identity=44.9367088607595, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17737937, Length=483, Percent_Identity=32.5051759834368, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24647201, Length=500, Percent_Identity=27.2, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24647199, Length=513, Percent_Identity=27.0955165692008, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24647197, Length=504, Percent_Identity=26.984126984127, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24647193, Length=517, Percent_Identity=26.1121856866538, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647195, Length=327, Percent_Identity=30.5810397553517, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR017611 [H]

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2 [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 52807; Mature: 52676

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVNIRNEIRFILNGRDVVLSSVAPDATLLDWLRLDRSLRGTKEGCAEGDCGACTVLVG
CCEECCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RLSAGGLIYESVNACIRFLGSLDGTHVVTVEHLRGQPGQLHPVQQAMVDFHGSQCGFCTP
HHCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHCCCCCCCCCH
GFVMSLYGLWMKSPDPSNAAIEKALQGNLCRCTGYEAIMRAAHAISSYGKAAKDPLAVER
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCCCCCHHHHH
KAITARLEALRDGARVEIGSAKARLIVPANVDDFATVLDQEPGATIVAGSTDVGLWVTKH
HHHHHHHHHHHCCCEEEECCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCCCHHHHHH
MRDISPAVFIGNLDGLCTVSEDKGVISIGAGVTYTEAFSMLAKRIPALGPLFDRIGGEQV
HHHCCCEEEEECCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHH
RNMGTIGGNIANGSPIGDTPPPLIALGARLTLRKGQKRRTIPLEAFFIAYGRQDRQPGEF
HHCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCHH
VEAVHVPVPAKGEKFAVYKITKRRDEDITAALGAFLLTLAKDGTVADVRIAYGGMAATPK
HHHEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCH
RASFVEKALLGKPWTEETVETAMAQYAGDFTPLTDMRASAEYRALAAKNLLLRFFVETTG
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC
TRAPFQVSRNEAA
CCCCEEECCCCCC
>Mature Secondary Structure 
AKVNIRNEIRFILNGRDVVLSSVAPDATLLDWLRLDRSLRGTKEGCAEGDCGACTVLVG
CEECCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RLSAGGLIYESVNACIRFLGSLDGTHVVTVEHLRGQPGQLHPVQQAMVDFHGSQCGFCTP
HHCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHCCCCCCCCCH
GFVMSLYGLWMKSPDPSNAAIEKALQGNLCRCTGYEAIMRAAHAISSYGKAAKDPLAVER
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCCCCCHHHHH
KAITARLEALRDGARVEIGSAKARLIVPANVDDFATVLDQEPGATIVAGSTDVGLWVTKH
HHHHHHHHHHHCCCEEEECCCCEEEEEECCCHHHHHHHCCCCCCEEEECCCCCCHHHHHH
MRDISPAVFIGNLDGLCTVSEDKGVISIGAGVTYTEAFSMLAKRIPALGPLFDRIGGEQV
HHHCCCEEEEECCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHH
RNMGTIGGNIANGSPIGDTPPPLIALGARLTLRKGQKRRTIPLEAFFIAYGRQDRQPGEF
HHCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCHH
VEAVHVPVPAKGEKFAVYKITKRRDEDITAALGAFLLTLAKDGTVADVRIAYGGMAATPK
HHHEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCH
RASFVEKALLGKPWTEETVETAMAQYAGDFTPLTDMRASAEYRALAAKNLLLRFFVETTG
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC
TRAPFQVSRNEAA
CCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]