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The map label for this gene is hisF

Identifier: 13474186

GI number: 13474186

Start: 3974162

End: 3974953

Strand: Direct

Name: hisF

Synonym: mlr5016

Alternate gene names: 13474186

Gene position: 3974162-3974953 (Clockwise)

Preceding gene: 13474185

Following gene: 13474187

Centisome position: 56.48

GC content: 66.67

Gene sequence:

>792_bases
ATGCTGAAAGCCCGTGTCATCCCGTGCCTCGACGTCAAGGACGGCCGCGTCGTCAAGGGCGTCAACTTCGTCGACCTCAT
CGACGCCGGCGACCCGGTCGAAGCGGCCAAGGCCTATGACGCCGCCGGCGCCGACGAACTCTGCTTTCTCGACATCACCG
CCTCGTCCGACAACCGTGAAACCATCTTCGATGTCATCGCCCGCACCGCCGAACAGTGCTTCATGCCGCTGACCGTCGGC
GGCGGCGTGCGCCAGGTCGCCGACATCAGGAAGCTGCTTCTGGCCGGCGCCGACAAGGTGTCGATCAACACGGCGGCGGT
GAAGAATCCGGATTTCGTCGCCGAAGCCGCCGACAAGTTCGGCAATCAATGCATCGTCGTCGCCATCGACGCCAAGAAGG
TCTCCGGCGCCGGCGAGGCCGACCGCTGGGAGATCTTCACCCATGGCGGACGCGAGAAGACCGGCATCGACGCCGTCGAA
TTCGCCCGCAAAATGGTCGATCGCGGCGCCGGCGAGATCCTGCTGACATCGATGGACCGCGACGGCACCAAGGCCGGCTA
CGATATCGCGCTGACGCGTGCGATCGCCGATGCGGTGCGCGCGCCGATCATCGCTTCGGGCGGCGTCGGCACGCTGGATC
ACCTGGTCGAAGGCATTCGCGACGGCCACGCCGGCGCGGTGCTGGCTGCCTCCATCTTCCATTTTGGCACCTACACCATC
GCGCAGGCCAAGGCGCATATGGCCGCCGCGGGCCTGCCGATGCGGCTGGACTCCTCGGGCGATCGGCCCTAG

Upstream 100 bases:

>100_bases
CCGTTTCCGCTTACGATCCGGCCTTCGACGCGCGGGGTGACGTCCCGCCGTTGGTCGGCGAGCTGCTCAACGATCTCATC
GCCACGATAGAGGGCCGCTG

Downstream 100 bases:

>100_bases
AGCCTGTCCGGCTAAGAAAAGGGCCCAGGCGCCATGGCTGAATTTTCGTTCTCCGATCTGGAGGCAATCATCAGGGACCG
CGCCCATTCCGGCGACCCGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRETIFDVIARTAEQCFMPLTVG
GGVRQVADIRKLLLAGADKVSINTAAVKNPDFVAEAADKFGNQCIVVAIDAKKVSGAGEADRWEIFTHGGREKTGIDAVE
FARKMVDRGAGEILLTSMDRDGTKAGYDIALTRAIADAVRAPIIASGGVGTLDHLVEGIRDGHAGAVLAASIFHFGTYTI
AQAKAHMAAAGLPMRLDSSGDRP

Sequences:

>Translated_263_residues
MLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRETIFDVIARTAEQCFMPLTVG
GGVRQVADIRKLLLAGADKVSINTAAVKNPDFVAEAADKFGNQCIVVAIDAKKVSGAGEADRWEIFTHGGREKTGIDAVE
FARKMVDRGAGEILLTSMDRDGTKAGYDIALTRAIADAVRAPIIASGGVGTLDHLVEGIRDGHAGAVLAASIFHFGTYTI
AQAKAHMAAAGLPMRLDSSGDRP
>Mature_263_residues
MLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRETIFDVIARTAEQCFMPLTVG
GGVRQVADIRKLLLAGADKVSINTAAVKNPDFVAEAADKFGNQCIVVAIDAKKVSGAGEADRWEIFTHGGREKTGIDAVE
FARKMVDRGAGEILLTSMDRDGTKAGYDIALTRAIADAVRAPIIASGGVGTLDHLVEGIRDGHAGAVLAASIFHFGTYTI
AQAKAHMAAAGLPMRLDSSGDRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=41.5384615384615, Blast_Score=203, Evalue=9e-54,
Organism=Escherichia coli, GI87082028, Length=246, Percent_Identity=29.2682926829268, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=31.4285714285714, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_RHILO (Q98CT1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105754.1
- ProteinModelPortal:   Q98CT1
- SMR:   Q98CT1
- GeneID:   1228415
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5016
- NMPDR:   fig|266835.1.peg.3858
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27569; Mature: 27569

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRE
CCCCCEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHH
TIFDVIARTAEQCFMPLTVGGGVRQVADIRKLLLAGADKVSINTAAVKNPDFVAEAADKF
HHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
GNQCIVVAIDAKKVSGAGEADRWEIFTHGGREKTGIDAVEFARKMVDRGAGEILLTSMDR
CCEEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTKAGYDIALTRAIADAVRAPIIASGGVGTLDHLVEGIRDGHAGAVLAASIFHFGTYTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AQAKAHMAAAGLPMRLDSSGDRP
HHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure
MLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAAKAYDAAGADELCFLDITASSDNRE
CCCCCEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHH
TIFDVIARTAEQCFMPLTVGGGVRQVADIRKLLLAGADKVSINTAAVKNPDFVAEAADKF
HHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
GNQCIVVAIDAKKVSGAGEADRWEIFTHGGREKTGIDAVEFARKMVDRGAGEILLTSMDR
CCEEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
DGTKAGYDIALTRAIADAVRAPIIASGGVGTLDHLVEGIRDGHAGAVLAASIFHFGTYTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AQAKAHMAAAGLPMRLDSSGDRP
HHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968