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The map label for this gene is nodE [H]

Identifier: 13474137

GI number: 13474137

Start: 3925595

End: 3926803

Strand: Direct

Name: nodE [H]

Synonym: mlr4953

Alternate gene names: 13474137

Gene position: 3925595-3926803 (Clockwise)

Preceding gene: 13474136

Following gene: 13474138

Centisome position: 55.79

GC content: 65.84

Gene sequence:

>1209_bases
ATGCTGAAACGCGTCGTCATTACCGGCATTGGCGGGCTGTGCGGACTAGGCGTCGATGCGCAGTCGATCTGGGCCGAAAT
GCGGGCCGGCCGTTCAGCCATAGGCCCGATCGACAATCCCTTCCTGCATGATTTGAAGGTCAAGACCGGCTGCGAGATCA
AGGCGCTGCCCGATCATGGCATCGAGCGCAAGCAGCTGGTGTCGATGGACCGTTTCAGCCTGCTGGCGGTGATCGCTGCT
AAAGAGGCCATGCGGCAGTCCGGCCTGGCCCCGCATGCCGACAACACCTATCGCATGGGAACCATCGTCGGCGTCGGCGT
CTGCGGCTTCGAGACCGTCGAGGAGAACTATCGCGCCATTCTGGTCGAGGGCAAGAATCGCGCCGGCATCTTCACCGTGC
CCAGGGTCATGCCGGGCGCCGCCGCCGGTCAGGTCAGCATGCATCTTGGCCTGCGCGGACCGGTCTTCGGCGTCACGTCG
GCCTGCTCGTCGGCCAACCACGCCATCGCCTCGGCGGCCGACCAGATCAGGCTCGGCCGCGCCGACGTCATGGTTGCCGG
CGGCACCGAAGCGCCACTGGTCTGGGGCGTGCTCAAGGGCTGGGAGGCATTGCGCGTGCTGTCACCCGACACCTGCCGGC
CGTTCTCGGCCGACCGCCAGGGCCTCGTGCTCGGCGAAGGTGCTGGCATGGCGGTTCTGGAAAGCTACGACCACGCCGTG
GCGCGTGGCGCCACCATCCTCGCCGAAATCGCCGGCGCCGGCCTTTCGGCCGATGCCTCCGACATCGTCGCCCCGACCAT
CGAGGGACCGGAAGCGGCGATGCGCTTCTGTCTCGTCGATGCCGGGCTCAATCCCGAGGACATCGATTATCTCAACGCGC
ATGGTACCGGCACCAAGGCCAACGACCAGATCGAGACCAACGCGATCAAGCGCGTCTTCGGCGATTACGCGCGGTCGCTG
TCCATCTCCTCGACCAAGTCGATGCATGCCCATTGCCTGGGCGCGTCGGGCGGGCTGGAGATGATCGCCTGCGTCATGGC
GATCCGCGAAGGCGTCGTGCCGCCGACCGCGAATTACCGCGAACCCGATCCCGATTGCGATCTCGACGTGACGCCCAATG
TCGCGCGCGAGCGCAAGGTGCGCGTCGCGATCAGCAACGGTTTTGCTTTCGGCGGCACCAACGCAGTGCTGGCATTCAAG
GCCATCTAA

Upstream 100 bases:

>100_bases
GAGATCGAGATGAACACGGTCGATGCCTGGAGCAATCTGAAAAATGTTGGCGACATGGTCGAGGCCGTTCGCGCGCTGAT
CGCGAAAAAAGCCTGACCGA

Downstream 100 bases:

>100_bases
TCCCGGGCGGTCACTACCGCCCCGGGCGGTCACCAGCGCCCGAGGGATTAACGTCTGCCGGGGCGGTTTGAGCGCCCTTT
GGCATTGCGCGTCCCGCTGT

Product: NodE

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLKRVVITGIGGLCGLGVDAQSIWAEMRAGRSAIGPIDNPFLHDLKVKTGCEIKALPDHGIERKQLVSMDRFSLLAVIAA
KEAMRQSGLAPHADNTYRMGTIVGVGVCGFETVEENYRAILVEGKNRAGIFTVPRVMPGAAAGQVSMHLGLRGPVFGVTS
ACSSANHAIASAADQIRLGRADVMVAGGTEAPLVWGVLKGWEALRVLSPDTCRPFSADRQGLVLGEGAGMAVLESYDHAV
ARGATILAEIAGAGLSADASDIVAPTIEGPEAAMRFCLVDAGLNPEDIDYLNAHGTGTKANDQIETNAIKRVFGDYARSL
SISSTKSMHAHCLGASGGLEMIACVMAIREGVVPPTANYREPDPDCDLDVTPNVARERKVRVAISNGFAFGGTNAVLAFK
AI

Sequences:

>Translated_402_residues
MLKRVVITGIGGLCGLGVDAQSIWAEMRAGRSAIGPIDNPFLHDLKVKTGCEIKALPDHGIERKQLVSMDRFSLLAVIAA
KEAMRQSGLAPHADNTYRMGTIVGVGVCGFETVEENYRAILVEGKNRAGIFTVPRVMPGAAAGQVSMHLGLRGPVFGVTS
ACSSANHAIASAADQIRLGRADVMVAGGTEAPLVWGVLKGWEALRVLSPDTCRPFSADRQGLVLGEGAGMAVLESYDHAV
ARGATILAEIAGAGLSADASDIVAPTIEGPEAAMRFCLVDAGLNPEDIDYLNAHGTGTKANDQIETNAIKRVFGDYARSL
SISSTKSMHAHCLGASGGLEMIACVMAIREGVVPPTANYREPDPDCDLDVTPNVARERKVRVAISNGFAFGGTNAVLAFK
AI
>Mature_402_residues
MLKRVVITGIGGLCGLGVDAQSIWAEMRAGRSAIGPIDNPFLHDLKVKTGCEIKALPDHGIERKQLVSMDRFSLLAVIAA
KEAMRQSGLAPHADNTYRMGTIVGVGVCGFETVEENYRAILVEGKNRAGIFTVPRVMPGAAAGQVSMHLGLRGPVFGVTS
ACSSANHAIASAADQIRLGRADVMVAGGTEAPLVWGVLKGWEALRVLSPDTCRPFSADRQGLVLGEGAGMAVLESYDHAV
ARGATILAEIAGAGLSADASDIVAPTIEGPEAAMRFCLVDAGLNPEDIDYLNAHGTGTKANDQIETNAIKRVFGDYARSL
SISSTKSMHAHCLGASGGLEMIACVMAIREGVVPPTANYREPDPDCDLDVTPNVARERKVRVAISNGFAFGGTNAVLAFK
AI

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=414, Percent_Identity=38.1642512077295, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI223972627, Length=175, Percent_Identity=40, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI41872631, Length=414, Percent_Identity=28.9855072463768, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1787337, Length=414, Percent_Identity=39.6135265700483, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788663, Length=415, Percent_Identity=33.9759036144578, Blast_Score=201, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI71983541, Length=420, Percent_Identity=32.1428571428571, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI71983535, Length=265, Percent_Identity=35.8490566037736, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17550940, Length=228, Percent_Identity=28.5087719298246, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI212642053, Length=346, Percent_Identity=27.7456647398844, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320904, Length=443, Percent_Identity=31.1512415349887, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24644341, Length=411, Percent_Identity=39.1727493917275, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI221330659, Length=352, Percent_Identity=29.8295454545455, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19920632, Length=352, Percent_Identity=29.8295454545455, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581345, Length=362, Percent_Identity=26.5193370165746, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 42076; Mature: 42076

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00098 THIOLASE_1 ; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRVVITGIGGLCGLGVDAQSIWAEMRAGRSAIGPIDNPFLHDLKVKTGCEIKALPDHG
CCCEEEEECCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHEEECCCCEEEECCCCC
IERKQLVSMDRFSLLAVIAAKEAMRQSGLAPHADNTYRMGTIVGVGVCGFETVEENYRAI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHHHCCCCEEE
LVEGKNRAGIFTVPRVMPGAAAGQVSMHLGLRGPVFGVTSACSSANHAIASAADQIRLGR
EEECCCCCCEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCC
ADVMVAGGTEAPLVWGVLKGWEALRVLSPDTCRPFSADRQGLVLGEGAGMAVLESYDHAV
CEEEEECCCCCCHHHHHHHCHHHEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
ARGATILAEIAGAGLSADASDIVAPTIEGPEAAMRFCLVDAGLNPEDIDYLNAHGTGTKA
HHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHEEECCCCCCCCCEEECCCCCCCC
NDQIETNAIKRVFGDYARSLSISSTKSMHAHCLGASGGLEMIACVMAIREGVVPPTANYR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCC
EPDPDCDLDVTPNVARERKVRVAISNGFAFGGTNAVLAFKAI
CCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCEEEEEEEC
>Mature Secondary Structure
MLKRVVITGIGGLCGLGVDAQSIWAEMRAGRSAIGPIDNPFLHDLKVKTGCEIKALPDHG
CCCEEEEECCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHEEECCCCEEEECCCCC
IERKQLVSMDRFSLLAVIAAKEAMRQSGLAPHADNTYRMGTIVGVGVCGFETVEENYRAI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHHHCCCCEEE
LVEGKNRAGIFTVPRVMPGAAAGQVSMHLGLRGPVFGVTSACSSANHAIASAADQIRLGR
EEECCCCCCEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCC
ADVMVAGGTEAPLVWGVLKGWEALRVLSPDTCRPFSADRQGLVLGEGAGMAVLESYDHAV
CEEEEECCCCCCHHHHHHHCHHHEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
ARGATILAEIAGAGLSADASDIVAPTIEGPEAAMRFCLVDAGLNPEDIDYLNAHGTGTKA
HHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHEEECCCCCCCCCEEECCCCCCCC
NDQIETNAIKRVFGDYARSLSISSTKSMHAHCLGASGGLEMIACVMAIREGVVPPTANYR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCC
EPDPDCDLDVTPNVARERKVRVAISNGFAFGGTNAVLAFKAI
CCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]