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The map label for this gene is dadB

Identifier: 13473409

GI number: 13473409

Start: 3195744

End: 3196889

Strand: Reverse

Name: dadB

Synonym: mll3997

Alternate gene names: 13473409

Gene position: 3196889-3195744 (Counterclockwise)

Preceding gene: 13473410

Following gene: 13473408

Centisome position: 45.44

GC content: 68.5

Gene sequence:

>1146_bases
ATGAACGACGCCGTCAATCCGACTGCTGCGAAAACCGTCACCGGTTGGCCGGTCAGCGAGGCCGCGGCCGGCGCGATCCT
GACCATCGATCTCGGCGCGATCCGTGAAAACTACCGGCGGCTGAAGGCGCTGCTGGGCGGCGTGCACTGCGCCGGCGTTG
TCAAGGCCAATGGCTACGGCCTTGGCGCGGCCAAGGTGGCGGCGGCACTGACCCGGGAAGGCTGTGACATCTTCTTCGTC
GCGCTGCTGGCCGAAGGCATCGCGCTGCGCAAGGCGGTCGGGACCGGACCGGACATCTACGTGCTGAACGGGCTGCCGCC
TGGCTCCGAGCCGGAAGCGGTGGCGGCTGGCCTTTGCGCGGTCATCAATAGTGGCGCGCAGTTGAAAGCCTGGCGCGCGG
CGGTGCACGATGCCGGTCGCCGGCTGCCGGCGGCCATCCAGGTCGACAGCGGCATGTCGCGGCTCGGCATGGCGCCGGCC
GAAGTCGAGGCGCTGGCCGGGGATAGCAGCGCGTTCGACGGCATCGACATCAAATATGTCATGAGCCATCTGGCCTGCGC
CGACGAGCCGCGCCATCCGGCCAACGAACAGCAGAGGCTGGCCTTCGAACGGCTGCGCGCCATGCTGCCCCGGGCGCCCG
CCTCGCTCGCCAATTCCTCCGGCATCTTTCTCGGGCCGTCCTACCATCACGATCTCGCTCGGCCCGGAGCCGCGCTCTAC
GGCATCAACCCGACGCCGGGCGAGCCCAACCCGATGTTGCCCGTGGTGTGGCTGCAGGCCAAGGTGGCGCAGACGCGCCG
CATCGAAAAGGGCGCCGGCATCGGCTATGGTCACAGCTATCACGCGGACGGTCCGCTGAGCCTGGCGACGATTTCGTTCG
GCTATGCGGATGGCTGGCTGCGCCGCTCCGCTTCGGCGGCCTGGTTCGAAGGCGTGCGCCTGCCTTTCCTCGGGCGTGTG
TCGATGGATTCGATTATTCTCGACATCTCCGCTTTGCCGCCCGGCAGGCTGCGCGAGGGCGATCTGGTCGAGCTGCTCGG
TCCCTCGCAGAGCGTCGACGACGCCGCCGGCCATGCCGGCACCATCGGCTATGAAATCCTGGCCAGTCTCGGCCCGCGCT
TTCACCGCCACTATGTCGGCGGCTGA

Upstream 100 bases:

>100_bases
AGAGGTGGTGGCGGCGTTCAGCCATCTGGCGCGTTAGCCGCGTCGAGGTTGCCGCGTTGGTGCGGGGGTCGTATTGATGG
CCGATGCAATGAGGGTCGCC

Downstream 100 bases:

>100_bases
GCGGTTTGGCCGCGCTTGACAAGCGGGCGCCTGTCGGCAAGGTTCGGGGCATGAGCAACCTCGCTTACACCAGAACCTTG
TTCAGGGTCTGCCCGATCGC

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNDAVNPTAAKTVTGWPVSEAAAGAILTIDLGAIRENYRRLKALLGGVHCAGVVKANGYGLGAAKVAAALTREGCDIFFV
ALLAEGIALRKAVGTGPDIYVLNGLPPGSEPEAVAAGLCAVINSGAQLKAWRAAVHDAGRRLPAAIQVDSGMSRLGMAPA
EVEALAGDSSAFDGIDIKYVMSHLACADEPRHPANEQQRLAFERLRAMLPRAPASLANSSGIFLGPSYHHDLARPGAALY
GINPTPGEPNPMLPVVWLQAKVAQTRRIEKGAGIGYGHSYHADGPLSLATISFGYADGWLRRSASAAWFEGVRLPFLGRV
SMDSIILDISALPPGRLREGDLVELLGPSQSVDDAAGHAGTIGYEILASLGPRFHRHYVGG

Sequences:

>Translated_381_residues
MNDAVNPTAAKTVTGWPVSEAAAGAILTIDLGAIRENYRRLKALLGGVHCAGVVKANGYGLGAAKVAAALTREGCDIFFV
ALLAEGIALRKAVGTGPDIYVLNGLPPGSEPEAVAAGLCAVINSGAQLKAWRAAVHDAGRRLPAAIQVDSGMSRLGMAPA
EVEALAGDSSAFDGIDIKYVMSHLACADEPRHPANEQQRLAFERLRAMLPRAPASLANSSGIFLGPSYHHDLARPGAALY
GINPTPGEPNPMLPVVWLQAKVAQTRRIEKGAGIGYGHSYHADGPLSLATISFGYADGWLRRSASAAWFEGVRLPFLGRV
SMDSIILDISALPPGRLREGDLVELLGPSQSVDDAAGHAGTIGYEILASLGPRFHRHYVGG
>Mature_381_residues
MNDAVNPTAAKTVTGWPVSEAAAGAILTIDLGAIRENYRRLKALLGGVHCAGVVKANGYGLGAAKVAAALTREGCDIFFV
ALLAEGIALRKAVGTGPDIYVLNGLPPGSEPEAVAAGLCAVINSGAQLKAWRAAVHDAGRRLPAAIQVDSGMSRLGMAPA
EVEALAGDSSAFDGIDIKYVMSHLACADEPRHPANEQQRLAFERLRAMLPRAPASLANSSGIFLGPSYHHDLARPGAALY
GINPTPGEPNPMLPVVWLQAKVAQTRRIEKGAGIGYGHSYHADGPLSLATISFGYADGWLRRSASAAWFEGVRLPFLGRV
SMDSIILDISALPPGRLREGDLVELLGPSQSVDDAAGHAGTIGYEILASLGPRFHRHYVGG

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1790487, Length=365, Percent_Identity=31.2328767123288, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787439, Length=355, Percent_Identity=30.9859154929577, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR2_RHILO (Q98F05)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104976.1
- ProteinModelPortal:   Q98F05
- SMR:   Q98F05
- GeneID:   1227637
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll3997
- NMPDR:   fig|266835.1.peg.3080
- HOGENOM:   HBG712172
- OMA:   RIADEFP
- BRENDA:   5.1.1.1
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 39785; Mature: 39785

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 55-55 ACT_SITE 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAVNPTAAKTVTGWPVSEAAAGAILTIDLGAIRENYRRLKALLGGVHCAGVVKANGYG
CCCCCCCCCCCEECCCCCHHHHCCEEEEEEHHHHHHHHHHHHHHHCCHHEEEEEEECCCC
LGAAKVAAALTREGCDIFFVALLAEGIALRKAVGTGPDIYVLNGLPPGSEPEAVAAGLCA
CCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHH
VINSGAQLKAWRAAVHDAGRRLPAAIQVDSGMSRLGMAPAEVEALAGDSSAFDGIDIKYV
HHCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHCCCCCCCCCCHHHHH
MSHLACADEPRHPANEQQRLAFERLRAMLPRAPASLANSSGIFLGPSYHHDLARPGAALY
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCCCHHHCCCCCEEE
GINPTPGEPNPMLPVVWLQAKVAQTRRIEKGAGIGYGHSYHADGPLSLATISFGYADGWL
ECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHH
RRSASAAWFEGVRLPFLGRVSMDSIILDISALPPGRLREGDLVELLGPSQSVDDAAGHAG
HHCCCHHHHCCCCCCEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHCCCCC
TIGYEILASLGPRFHRHYVGG
HHHHHHHHHHCHHHHHHCCCC
>Mature Secondary Structure
MNDAVNPTAAKTVTGWPVSEAAAGAILTIDLGAIRENYRRLKALLGGVHCAGVVKANGYG
CCCCCCCCCCCEECCCCCHHHHCCEEEEEEHHHHHHHHHHHHHHHCCHHEEEEEEECCCC
LGAAKVAAALTREGCDIFFVALLAEGIALRKAVGTGPDIYVLNGLPPGSEPEAVAAGLCA
CCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHH
VINSGAQLKAWRAAVHDAGRRLPAAIQVDSGMSRLGMAPAEVEALAGDSSAFDGIDIKYV
HHCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHCCCCCCCCCCHHHHH
MSHLACADEPRHPANEQQRLAFERLRAMLPRAPASLANSSGIFLGPSYHHDLARPGAALY
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCCCCHHHCCCCCEEE
GINPTPGEPNPMLPVVWLQAKVAQTRRIEKGAGIGYGHSYHADGPLSLATISFGYADGWL
ECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHH
RRSASAAWFEGVRLPFLGRVSMDSIILDISALPPGRLREGDLVELLGPSQSVDDAAGHAG
HHCCCHHHHCCCCCCEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCHHHCCCCC
TIGYEILASLGPRFHRHYVGG
HHHHHHHHHHCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968