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The map label for this gene is phoB [H]

Identifier: 13473197

GI number: 13473197

Start: 2994399

End: 2995088

Strand: Reverse

Name: phoB [H]

Synonym: mll3716

Alternate gene names: 13473197

Gene position: 2995088-2994399 (Counterclockwise)

Preceding gene: 13473198

Following gene: 13473195

Centisome position: 42.57

GC content: 64.2

Gene sequence:

>690_bases
ATGATCGCACCACGCATCATGGTGGTGGAGGACGAGGAGCCGCTCGGCGTGCTCCTCCGCTACAATCTGGAATCGGAGGG
TTACCAGGTCGAGGTGGTGACACGCGGCGACGAAGCCGAAATCCGCCTGCAGGAGAACGTGCCCGATCTCCTGGTGCTCG
ACTGGATGGTGCCGGCGGTGTCCGGCATCGAACTCTGCCGGCGCCTGCGGATGCGGCCCGAGACCGAGCGGCTGCCGATC
ATCATGCTGACCGCGCGGGGCGAGGAAAGCGACCGTGTGCGTGGGCTCTCCACCGGCGCCGACGACTATCTGGTCAAGCC
GTTTTCGACGCCGGAATTCATGGCTCGCGTGAAGGCGCTGCTACGCCGCGCCAAGCCGGAAGTGCTGTCCAGCGTGCTCA
AGGTCGGCGACATCGTGCTCGACCGTGAATCACACCGGGTCTATCGCAAAAAGAGCGAGATCCGGCTCGGGCCGACTGAA
TTCCGCCTGCTCGAATTCATGATGCGTCATCCCGGCCGCGTGTTCTCGCGCAGCCAGCTGCTCGACAATGTCTGGGGCGA
GACGATCTACATCGACGAGCGCACCGTGGACGTGCATGTCGGCCGCCTGCGCAAGGCGGTCAACAATGGCCGCATGCCGG
ATGTTATCAGAACCATTCGTGGGGCGGGCTACGCGATCCGCGAGGATTGA

Upstream 100 bases:

>100_bases
TACTATGTCCTGACCGGCACGCAACTGCCCGCCAACCGTCCAAAACAGGACGAGACGGCGATGTCGGCGCCGGCGGCCTG
AGCGCAGGGGTAACCAATCG

Downstream 100 bases:

>100_bases
GCACGACCTGAATCAGGCGACGTTTTTCTTCGCGGCAAGGCCTGCTTTCGTTTGCACGCCCGGCGCGAAAATCTCCTGGT
CGACAAAGGTCAGCGCGCGC

Product: phosphate regulatory protein, PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MIAPRIMVVEDEEPLGVLLRYNLESEGYQVEVVTRGDEAEIRLQENVPDLLVLDWMVPAVSGIELCRRLRMRPETERLPI
IMLTARGEESDRVRGLSTGADDYLVKPFSTPEFMARVKALLRRAKPEVLSSVLKVGDIVLDRESHRVYRKKSEIRLGPTE
FRLLEFMMRHPGRVFSRSQLLDNVWGETIYIDERTVDVHVGRLRKAVNNGRMPDVIRTIRGAGYAIRED

Sequences:

>Translated_229_residues
MIAPRIMVVEDEEPLGVLLRYNLESEGYQVEVVTRGDEAEIRLQENVPDLLVLDWMVPAVSGIELCRRLRMRPETERLPI
IMLTARGEESDRVRGLSTGADDYLVKPFSTPEFMARVKALLRRAKPEVLSSVLKVGDIVLDRESHRVYRKKSEIRLGPTE
FRLLEFMMRHPGRVFSRSQLLDNVWGETIYIDERTVDVHVGRLRKAVNNGRMPDVIRTIRGAGYAIRED
>Mature_229_residues
MIAPRIMVVEDEEPLGVLLRYNLESEGYQVEVVTRGDEAEIRLQENVPDLLVLDWMVPAVSGIELCRRLRMRPETERLPI
IMLTARGEESDRVRGLSTGADDYLVKPFSTPEFMARVKALLRRAKPEVLSSVLKVGDIVLDRESHRVYRKKSEIRLGPTE
FRLLEFMMRHPGRVFSRSQLLDNVWGETIYIDERTVDVHVGRLRKAVNNGRMPDVIRTIRGAGYAIRED

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=48.8789237668161, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=41.8502202643172, Blast_Score=160, Evalue=4e-41,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=39.1891891891892, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=37.9464285714286, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=35.7758620689655, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.7713004484305, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=35.5555555555556, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=35, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1789402, Length=224, Percent_Identity=34.375, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=35.5855855855856, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=36.480686695279, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790863, Length=241, Percent_Identity=32.7800829875519, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1787229, Length=231, Percent_Identity=32.9004329004329, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=31.7596566523605, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=30.2752293577982, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26369; Mature: 26369

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPRIMVVEDEEPLGVLLRYNLESEGYQVEVVTRGDEAEIRLQENVPDLLVLDWMVPAV
CCCCEEEEEECCCCCEEEEEEEECCCCEEEEEEECCCCCEEEECCCCCCEEHHHHHHHHH
SGIELCRRLRMRPETERLPIIMLTARGEESDRVRGLSTGADDYLVKPFSTPEFMARVKAL
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHEECCCCCCCCEEECCCCCHHHHHHHHHH
LRRAKPEVLSSVLKVGDIVLDRESHRVYRKKSEIRLGPTEFRLLEFMMRHPGRVFSRSQL
HHHCCHHHHHHHHHHHHHEECCCHHHHHHHHCCCEECHHHHHHHHHHHHCCCCHHHHHHH
LDNVWGETIYIDERTVDVHVGRLRKAVNNGRMPDVIRTIRGAGYAIRED
HHHHCCCEEEEECCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MIAPRIMVVEDEEPLGVLLRYNLESEGYQVEVVTRGDEAEIRLQENVPDLLVLDWMVPAV
CCCCEEEEEECCCCCEEEEEEEECCCCEEEEEEECCCCCEEEECCCCCCEEHHHHHHHHH
SGIELCRRLRMRPETERLPIIMLTARGEESDRVRGLSTGADDYLVKPFSTPEFMARVKAL
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHEECCCCCCCCEEECCCCCHHHHHHHHHH
LRRAKPEVLSSVLKVGDIVLDRESHRVYRKKSEIRLGPTEFRLLEFMMRHPGRVFSRSQL
HHHCCHHHHHHHHHHHHHEECCCHHHHHHHHCCCEECHHHHHHHHHHHHCCCCHHHHHHH
LDNVWGETIYIDERTVDVHVGRLRKAVNNGRMPDVIRTIRGAGYAIRED
HHHHCCCEEEEECCEEEHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]