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The map label for this gene is dapE [H]

Identifier: 13472867

GI number: 13472867

Start: 2646732

End: 2648117

Strand: Reverse

Name: dapE [H]

Synonym: mll3297

Alternate gene names: 13472867

Gene position: 2648117-2646732 (Counterclockwise)

Preceding gene: 13472869

Following gene: 13472866

Centisome position: 37.64

GC content: 63.28

Gene sequence:

>1386_bases
ATGTCCGCAGTTTCCCCCGTCCTCGACCGCCTCGACAAGAATTTCGACCAGAGCCTCGAACGCCTGTTCGGCCTGCTCAG
GATCAAGTCGATCTCCACCGATCCGGCCTATGCCGCCGACTGCCGCAAGGCGGCGGAATGGCTTGTCGCGGAGCTCAAGC
TTATCGGCTTCGACGCCAGCGTGCGCGACACGCCGGGACACCCGATGGTGGTTGCGCATCACGAGGGGCCGGCCGGTTCG
CCGCATGTGCTGTTCTATGGCCATTACGACGTGCAGCCCGTCGACCCGATCGAATTGTGGGAAAACGACCCGTTCGCGCC
TGCCGTCAAGGAGGTCGGACCCGACCACAAGGTGATCACCGGGCGCGGTTCGGCCGACGACAAGGGCCAGCTGATGACCT
TTGTCGAGGCTTGCCGCGCCTGGAAACAGGTGCATGGCAATCTGCCATGCCGCGTCACCATCCTGTTCGAAGGCGAGGAG
GAATCCGGCTCGCCATCGCTGATACCGTTCCTGAAGGCCAATGCCGACGAGCTCAAGGGCGACTTCGCGCTGGTCTGCGA
CACCGGGATGTGGGACCGCGAGACGCCCTCAATCTGCGTTTCCTTGCGCGGGATGGTCGGCGAGGAGGTCACGGTAAAGG
CCGCCAGCCGCGATCTGCATTCCGGGCTTTATGGCGGTGCCGCCGCCAACCCGATCCGTATTCTCGCGAAAATCCTGGCC
GACATCCATGACAAGGACGGCCACATCACCATTCCGGGTTTCTATGATGGCGTCGAGGAGACGCCAACACAGGTGCTGAA
ATCTTGGGAAAGTCTCGGCGAGACGGCCGAGACTTTCCTCGGCCCTGTCGGCCTGTCGATCCCTTCGGGTGAGAAAGGCC
GCTCGGTGTTGGAACTGACCTGGGCGCGGCCGACCGCCGAGTTCAACGGCATCATCGGCGGCTACACCGGCAAGGGCTTC
AAGACGGTGATCGCGGCGGAAGCCTCGGCCAAGGTGTCGTTCCGCCTCGTCCACAAGCAGGATCCGAAGGCAATCCGTGC
CGCGTTCCAGGCCTTCGTGCGGGAACGTATCCCAGCCGATTGCTCTGTCGAATTCCACCCGCATGGCGGCTCGCCGGCAA
TCCAGCTTTCCTATGACTCGCCGTTCCTGGCCAAGGCCAAGGACGCGCTGTCGGACGAATGGGAGAAACAGGCGGTCACG
ACCGGCAGTGGCGGATCAATCCCGGTTGTCGGCGATTTCCAGACCTATCTCGGTATCGAGTCCCTGCTGGTCGGCTTCGG
CCTCGACGACGACCGCATCCACTCGCCCAACGAGAAATACGAGCTGTCGTCCTTCCACAAGGGCCAGCGCTCCTGGGCGC
GGATTCTCGATGCACTGACGCGCTGA

Upstream 100 bases:

>100_bases
GCCTACGGCAGCCGACTGGCGCCGTGGTCCAATCGTCTCGGGCTTTTCGTTTCCCCGCTCTGCCTTTACGGTTCCGGCAC
TGAATCGAAAGGTCTCACAT

Downstream 100 bases:

>100_bases
GCGGCGGAAAAGACGTTTTCCTGTTGATTTTTCACCGGATCGCGGCGAGGACGGGGAACGCCGCGACCGGAGAAGACAAT
GGCCGACATCCGTTTCCACA

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSAVSPVLDRLDKNFDQSLERLFGLLRIKSISTDPAYAADCRKAAEWLVAELKLIGFDASVRDTPGHPMVVAHHEGPAGS
PHVLFYGHYDVQPVDPIELWENDPFAPAVKEVGPDHKVITGRGSADDKGQLMTFVEACRAWKQVHGNLPCRVTILFEGEE
ESGSPSLIPFLKANADELKGDFALVCDTGMWDRETPSICVSLRGMVGEEVTVKAASRDLHSGLYGGAAANPIRILAKILA
DIHDKDGHITIPGFYDGVEETPTQVLKSWESLGETAETFLGPVGLSIPSGEKGRSVLELTWARPTAEFNGIIGGYTGKGF
KTVIAAEASAKVSFRLVHKQDPKAIRAAFQAFVRERIPADCSVEFHPHGGSPAIQLSYDSPFLAKAKDALSDEWEKQAVT
TGSGGSIPVVGDFQTYLGIESLLVGFGLDDDRIHSPNEKYELSSFHKGQRSWARILDALTR

Sequences:

>Translated_461_residues
MSAVSPVLDRLDKNFDQSLERLFGLLRIKSISTDPAYAADCRKAAEWLVAELKLIGFDASVRDTPGHPMVVAHHEGPAGS
PHVLFYGHYDVQPVDPIELWENDPFAPAVKEVGPDHKVITGRGSADDKGQLMTFVEACRAWKQVHGNLPCRVTILFEGEE
ESGSPSLIPFLKANADELKGDFALVCDTGMWDRETPSICVSLRGMVGEEVTVKAASRDLHSGLYGGAAANPIRILAKILA
DIHDKDGHITIPGFYDGVEETPTQVLKSWESLGETAETFLGPVGLSIPSGEKGRSVLELTWARPTAEFNGIIGGYTGKGF
KTVIAAEASAKVSFRLVHKQDPKAIRAAFQAFVRERIPADCSVEFHPHGGSPAIQLSYDSPFLAKAKDALSDEWEKQAVT
TGSGGSIPVVGDFQTYLGIESLLVGFGLDDDRIHSPNEKYELSSFHKGQRSWARILDALTR
>Mature_460_residues
SAVSPVLDRLDKNFDQSLERLFGLLRIKSISTDPAYAADCRKAAEWLVAELKLIGFDASVRDTPGHPMVVAHHEGPAGSP
HVLFYGHYDVQPVDPIELWENDPFAPAVKEVGPDHKVITGRGSADDKGQLMTFVEACRAWKQVHGNLPCRVTILFEGEEE
SGSPSLIPFLKANADELKGDFALVCDTGMWDRETPSICVSLRGMVGEEVTVKAASRDLHSGLYGGAAANPIRILAKILAD
IHDKDGHITIPGFYDGVEETPTQVLKSWESLGETAETFLGPVGLSIPSGEKGRSVLELTWARPTAEFNGIIGGYTGKGFK
TVIAAEASAKVSFRLVHKQDPKAIRAAFQAFVRERIPADCSVEFHPHGGSPAIQLSYDSPFLAKAKDALSDEWEKQAVTT
GSGGSIPVVGDFQTYLGIESLLVGFGLDDDRIHSPNEKYELSSFHKGQRSWARILDALTR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=474, Percent_Identity=28.6919831223629, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI21071039, Length=395, Percent_Identity=27.8481012658228, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI271398277, Length=340, Percent_Identity=30, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17563146, Length=464, Percent_Identity=28.8793103448276, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193211059, Length=473, Percent_Identity=27.906976744186, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321159, Length=474, Percent_Identity=28.0590717299578, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319758, Length=468, Percent_Identity=23.0769230769231, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24585880, Length=474, Percent_Identity=29.746835443038, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50073; Mature: 49942

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVSPVLDRLDKNFDQSLERLFGLLRIKSISTDPAYAADCRKAAEWLVAELKLIGFDAS
CCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
VRDTPGHPMVVAHHEGPAGSPHVLFYGHYDVQPVDPIELWENDPFAPAVKEVGPDHKVIT
CCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHCCCCCEEEE
GRGSADDKGQLMTFVEACRAWKQVHGNLPCRVTILFEGEEESGSPSLIPFLKANADELKG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCHHHHCC
DFALVCDTGMWDRETPSICVSLRGMVGEEVTVKAASRDLHSGLYGGAAANPIRILAKILA
CEEEEECCCCCCCCCCHHHHEECCCCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHH
DIHDKDGHITIPGFYDGVEETPTQVLKSWESLGETAETFLGPVGLSIPSGEKGRSVLELT
HHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
WARPTAEFNGIIGGYTGKGFKTVIAAEASAKVSFRLVHKQDPKAIRAAFQAFVRERIPAD
ECCCCHHHCCEEECCCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCC
CSVEFHPHGGSPAIQLSYDSPFLAKAKDALSDEWEKQAVTTGSGGSIPVVGDFQTYLGIE
CEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCCEEEECHHHHHHHH
SLLVGFGLDDDRIHSPNEKYELSSFHKGQRSWARILDALTR
HHHHHCCCCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SAVSPVLDRLDKNFDQSLERLFGLLRIKSISTDPAYAADCRKAAEWLVAELKLIGFDAS
CHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
VRDTPGHPMVVAHHEGPAGSPHVLFYGHYDVQPVDPIELWENDPFAPAVKEVGPDHKVIT
CCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHCCCCCEEEE
GRGSADDKGQLMTFVEACRAWKQVHGNLPCRVTILFEGEEESGSPSLIPFLKANADELKG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCHHHHCC
DFALVCDTGMWDRETPSICVSLRGMVGEEVTVKAASRDLHSGLYGGAAANPIRILAKILA
CEEEEECCCCCCCCCCHHHHEECCCCCCCEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHH
DIHDKDGHITIPGFYDGVEETPTQVLKSWESLGETAETFLGPVGLSIPSGEKGRSVLELT
HHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
WARPTAEFNGIIGGYTGKGFKTVIAAEASAKVSFRLVHKQDPKAIRAAFQAFVRERIPAD
ECCCCHHHCCEEECCCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCC
CSVEFHPHGGSPAIQLSYDSPFLAKAKDALSDEWEKQAVTTGSGGSIPVVGDFQTYLGIE
CEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCCEEEECHHHHHHHH
SLLVGFGLDDDRIHSPNEKYELSSFHKGQRSWARILDALTR
HHHHHCCCCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA