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The map label for this gene is polA [H]

Identifier: 13472866

GI number: 13472866

Start: 2646033

End: 2646653

Strand: Reverse

Name: polA [H]

Synonym: mll3296

Alternate gene names: 13472866

Gene position: 2646653-2646033 (Counterclockwise)

Preceding gene: 13472867

Following gene: 13472865

Centisome position: 37.62

GC content: 64.25

Gene sequence:

>621_bases
ATGGCCGACATCCGTTTCCACAAGCATGATCTGCCCGACCTTTCGCATTACAATGTCGGGGCGGTCGCCATCGACACCGA
GACGCTGGGGCTGAACCCGCATCGCGACCGGTTGTGCTTGGTGCAGATATCGCCCGGCGACGGCAGTGCCGACGTCATCC
AGATCGCACCCGGCCAGAAGAAGGCGCCGAACCTCGTCAGCCTGCTCAGGAATCGCAGCGTGACGAAGCTGTTCCATTTC
GGACGCTTCGACCTTGCCGTGCTCTACAACGCCTTCGGGGTGATGCCCGAACCGGTGTTCTGCACCAAGATCGCCTCGCG
GCTGACCCGCACCTATACCGACCGGCACGGGCTGAAGGACATCTGCTTCGAGCTTCTCGGTGTCGGCCTGTCCAAGGCGC
AGCAATCGTCGGACTGGGCGGCCGAAACGCTGTCGCCCGAGCAGCTCGAATATGCCGCCTCGGACGTGCTCTATCTGCAC
CGGCTGCGCGATGTGCTGGCCGGGCGGCTGGCGCGCGAGGGGCGGACCAAGGAGGCCGATGCCTGCTTCCGCTTCCTGCC
GACGCGGGCCAAGCTTGACCTGATGGGCTGGGACGAAGAGGATATCTTCGCTCACAGCTGA

Upstream 100 bases:

>100_bases
TCTCGATGCACTGACGCGCTGAGCGGCGGAAAAGACGTTTTCCTGTTGATTTTTCACCGGATCGCGGCGAGGACGGGGAA
CGCCGCGACCGGAGAAGACA

Downstream 100 bases:

>100_bases
CCGCCTTCGCGACAATCCGCGCCCGACAGCTGGTTGGGAACCAGATGCCAGCCGTCGCGTTCTGTGGGCTGGAAAAAGGG
GATGACATGGCCGACAGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MADIRFHKHDLPDLSHYNVGAVAIDTETLGLNPHRDRLCLVQISPGDGSADVIQIAPGQKKAPNLVSLLRNRSVTKLFHF
GRFDLAVLYNAFGVMPEPVFCTKIASRLTRTYTDRHGLKDICFELLGVGLSKAQQSSDWAAETLSPEQLEYAASDVLYLH
RLRDVLAGRLAREGRTKEADACFRFLPTRAKLDLMGWDEEDIFAHS

Sequences:

>Translated_206_residues
MADIRFHKHDLPDLSHYNVGAVAIDTETLGLNPHRDRLCLVQISPGDGSADVIQIAPGQKKAPNLVSLLRNRSVTKLFHF
GRFDLAVLYNAFGVMPEPVFCTKIASRLTRTYTDRHGLKDICFELLGVGLSKAQQSSDWAAETLSPEQLEYAASDVLYLH
RLRDVLAGRLAREGRTKEADACFRFLPTRAKLDLMGWDEEDIFAHS
>Mature_205_residues
ADIRFHKHDLPDLSHYNVGAVAIDTETLGLNPHRDRLCLVQISPGDGSADVIQIAPGQKKAPNLVSLLRNRSVTKLFHFG
RFDLAVLYNAFGVMPEPVFCTKIASRLTRTYTDRHGLKDICFELLGVGLSKAQQSSDWAAETLSPEQLEYAASDVLYLHR
LRDVLAGRLAREGRTKEADACFRFLPTRAKLDLMGWDEEDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=140, Percent_Identity=35.7142857142857, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 23065; Mature: 22933

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIRFHKHDLPDLSHYNVGAVAIDTETLGLNPHRDRLCLVQISPGDGSADVIQIAPGQK
CCCCCCCCCCCCCCCCCCCCEEEEECEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCC
KAPNLVSLLRNRSVTKLFHFGRFDLAVLYNAFGVMPEPVFCTKIASRLTRTYTDRHGLKD
CCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
ICFELLGVGLSKAQQSSDWAAETLSPEQLEYAASDVLYLHRLRDVLAGRLAREGRTKEAD
HHHHHHHCCHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ACFRFLPTRAKLDLMGWDEEDIFAHS
HHHHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ADIRFHKHDLPDLSHYNVGAVAIDTETLGLNPHRDRLCLVQISPGDGSADVIQIAPGQK
CCCCCCCCCCCCCCCCCCCEEEEECEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCC
KAPNLVSLLRNRSVTKLFHFGRFDLAVLYNAFGVMPEPVFCTKIASRLTRTYTDRHGLKD
CCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHH
ICFELLGVGLSKAQQSSDWAAETLSPEQLEYAASDVLYLHRLRDVLAGRLAREGRTKEAD
HHHHHHHCCHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ACFRFLPTRAKLDLMGWDEEDIFAHS
HHHHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]