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The map label for this gene is ubiB [H]

Identifier: 13472763

GI number: 13472763

Start: 2535626

End: 2537200

Strand: Direct

Name: ubiB [H]

Synonym: mlr3166

Alternate gene names: 13472763

Gene position: 2535626-2537200 (Clockwise)

Preceding gene: 13472762

Following gene: 13472764

Centisome position: 36.04

GC content: 66.48

Gene sequence:

>1575_bases
ATGAGCAGCGTCGGCGCTGGTTTCAGGCTCGCGCGGGCCGGCTGGGTGCTGGTGCGCGAGGGCGTTATCGCCGCCCTACC
GGGCGAGGAACTGTCCGGTCTGCCGAAATTCGGCTGGCAACTGGCGCGGCTGTTCACCCGCCGCCGCGCGCTCGCCTATC
AGCGCAGCGACCGGCTGGCCAAGGCCGTGGTCCGGCTCGGCCCGTCCTATGTCAAGCTCGGCCAGTTCCTGGCAACACGG
CCCGACGTCGTCGGCAACGACATGGCGCTCGATCTCGCCATGTTGCAGGACAAGATGCACACCTTCCCGAAGGCCGAGGC
TGTCGCGGCCATCGAAGCCTCGCTCGGGCGCAAGGTGCGCGATCTCTACGCGGAGTTCGGCGACCCGGTCGCCGCCGCTT
CCATCGCCCAGGTCCATGCCGCCGAAGCCATCCATGATGGCGTTGCCACCAAGGTCGCGGTGAAGGTCATCCGGCCCGGT
GTGCGCCGCCGGTTCTTCCAGGATCTCGAAAGCTATTTCCTCGCCGCCCGCCTGCAGGAGAAGTACATCCCGTCCTCGCG
CCGGCTGCGGCCTGTCGAGGTGACCGAGACGCTGGCGCAGACCACCAAGATCGAGATGGATTTGCGCCTCGAGGCGGCGG
CACTCTCCGAACTTGGTGAGAACACCAAGGACGATCCGGGCTTCCGCGTGCCATCCGTCGACTGGGAACGCACCGGCCGC
GACGTGCTGACCATGGAATGGGTCGACGGCGTCAAGATGAACAACATCGCCGGCCTGGAGGAGGCCGGCCATGATCTGAA
GGCGATCGCCGCCAATCTCATCCAGTCGTTCCTGCGCCATACGCTGCGTGACGGTTTCTTCCACGCCGACATGCACCCCG
GAAACCTGTTCGTCGAAGCCAACGGCACCATCGTCGCCGTCGATCTCGGCATTGCCGGACGTCTCGGCAAGAAGGAGCGC
CGCTTCCTCGCCGAAATCCTCTACGGCTTCATCACGCGCGACTATCTGCGCGTCGCCGAAGTGCATTTCGAGGCCGGCTA
CGTGCCGCGCCAGCACAATGTCGCGGCCTTCGCGCAGGCGATTCGCGCCATCGGCGAGCCGATTCACGGCCAGCCAGCCG
AGACCATTTCCATGGCCAAGCTCCTGACGCTGCTGTTCGAGGTGACCGAACTCTTCGACATGGCGACACGGCCGGAGCTG
ATCCTGCTGCAGAAGACCATGGTCGTGGTCGAAGGCGTGGCGCGCACGCTCGATCCAGCCTTCAACATGTGGAAGACCGC
CGAGCCGGTGGTCGGCGGCTGGATCGCCGGAAACCTTGGCCCGCGCATCATGATGATCGATGCGCGCGACGGTGCCAAGG
CGCTGCTGACGCTGGCCCGCCAGGCGCCGGAGCTTGCCGCGCGCACCGACCGGCTGTCGCGCGAGATCGACCTGATGGCC
GAGCACGGCCTGCGTTTCGACGAGACCACGGCGCGTGCCATCGGCAAGGCCGAGGCGCGCTACAGCCGCTCCGGCCGCGT
CGCGCTGTGGGTGATCGCATTGACGCTGGTCTATATCGCCTGGAAGATTCTCTAG

Upstream 100 bases:

>100_bases
ATTGCCGCGCTTCATTCGGGCTGGAAGCTTTGAGGCTGGCCCGCATTTCTCATCCCCGATATGGCTCATGCCGGTCGCGA
GAACCGGCCTGGGTGCGGCC

Downstream 100 bases:

>100_bases
GCCTGGAAATCGAAGCTCGAGCTGACAACTTGCGGCGAGTTGATTGCATTGCTATCATTGCTATCGGTCAGCATGAGCAA
AGTGCAATCACATGGCAAGC

Product: ubiquinone biosynthesis protein AarF

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSSVGAGFRLARAGWVLVREGVIAALPGEELSGLPKFGWQLARLFTRRRALAYQRSDRLAKAVVRLGPSYVKLGQFLATR
PDVVGNDMALDLAMLQDKMHTFPKAEAVAAIEASLGRKVRDLYAEFGDPVAAASIAQVHAAEAIHDGVATKVAVKVIRPG
VRRRFFQDLESYFLAARLQEKYIPSSRRLRPVEVTETLAQTTKIEMDLRLEAAALSELGENTKDDPGFRVPSVDWERTGR
DVLTMEWVDGVKMNNIAGLEEAGHDLKAIAANLIQSFLRHTLRDGFFHADMHPGNLFVEANGTIVAVDLGIAGRLGKKER
RFLAEILYGFITRDYLRVAEVHFEAGYVPRQHNVAAFAQAIRAIGEPIHGQPAETISMAKLLTLLFEVTELFDMATRPEL
ILLQKTMVVVEGVARTLDPAFNMWKTAEPVVGGWIAGNLGPRIMMIDARDGAKALLTLARQAPELAARTDRLSREIDLMA
EHGLRFDETTARAIGKAEARYSRSGRVALWVIALTLVYIAWKIL

Sequences:

>Translated_524_residues
MSSVGAGFRLARAGWVLVREGVIAALPGEELSGLPKFGWQLARLFTRRRALAYQRSDRLAKAVVRLGPSYVKLGQFLATR
PDVVGNDMALDLAMLQDKMHTFPKAEAVAAIEASLGRKVRDLYAEFGDPVAAASIAQVHAAEAIHDGVATKVAVKVIRPG
VRRRFFQDLESYFLAARLQEKYIPSSRRLRPVEVTETLAQTTKIEMDLRLEAAALSELGENTKDDPGFRVPSVDWERTGR
DVLTMEWVDGVKMNNIAGLEEAGHDLKAIAANLIQSFLRHTLRDGFFHADMHPGNLFVEANGTIVAVDLGIAGRLGKKER
RFLAEILYGFITRDYLRVAEVHFEAGYVPRQHNVAAFAQAIRAIGEPIHGQPAETISMAKLLTLLFEVTELFDMATRPEL
ILLQKTMVVVEGVARTLDPAFNMWKTAEPVVGGWIAGNLGPRIMMIDARDGAKALLTLARQAPELAARTDRLSREIDLMA
EHGLRFDETTARAIGKAEARYSRSGRVALWVIALTLVYIAWKIL
>Mature_523_residues
SSVGAGFRLARAGWVLVREGVIAALPGEELSGLPKFGWQLARLFTRRRALAYQRSDRLAKAVVRLGPSYVKLGQFLATRP
DVVGNDMALDLAMLQDKMHTFPKAEAVAAIEASLGRKVRDLYAEFGDPVAAASIAQVHAAEAIHDGVATKVAVKVIRPGV
RRRFFQDLESYFLAARLQEKYIPSSRRLRPVEVTETLAQTTKIEMDLRLEAAALSELGENTKDDPGFRVPSVDWERTGRD
VLTMEWVDGVKMNNIAGLEEAGHDLKAIAANLIQSFLRHTLRDGFFHADMHPGNLFVEANGTIVAVDLGIAGRLGKKERR
FLAEILYGFITRDYLRVAEVHFEAGYVPRQHNVAAFAQAIRAIGEPIHGQPAETISMAKLLTLLFEVTELFDMATRPELI
LLQKTMVVVEGVARTLDPAFNMWKTAEPVVGGWIAGNLGPRIMMIDARDGAKALLTLARQAPELAARTDRLSREIDLMAE
HGLRFDETTARAIGKAEARYSRSGRVALWVIALTLVYIAWKIL

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI41393593, Length=289, Percent_Identity=31.1418685121107, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI40254938, Length=293, Percent_Identity=27.3037542662116, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI217035081, Length=236, Percent_Identity=29.6610169491525, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI27363457, Length=329, Percent_Identity=25.2279635258359, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI217416386, Length=276, Percent_Identity=27.8985507246377, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI32261307, Length=336, Percent_Identity=24.4047619047619, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI34147522, Length=304, Percent_Identity=25.3289473684211, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI2367309, Length=478, Percent_Identity=35.3556485355649, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17559152, Length=302, Percent_Identity=24.8344370860927, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325148, Length=454, Percent_Identity=24.6696035242291, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321319, Length=328, Percent_Identity=26.219512195122, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24662575, Length=320, Percent_Identity=27.8125, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI22024280, Length=256, Percent_Identity=25.78125, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 57938; Mature: 57807

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVGAGFRLARAGWVLVREGVIAALPGEELSGLPKFGWQLARLFTRRRALAYQRSDRLA
CCCCCCCHHHHHHCCCEEECCEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAVVRLGPSYVKLGQFLATRPDVVGNDMALDLAMLQDKMHTFPKAEAVAAIEASLGRKVR
HHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
DLYAEFGDPVAAASIAQVHAAEAIHDGVATKVAVKVIRPGVRRRFFQDLESYFLAARLQE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYIPSSRRLRPVEVTETLAQTTKIEMDLRLEAAALSELGENTKDDPGFRVPSVDWERTGR
HHCCCCCCCCCHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
DVLTMEWVDGVKMNNIAGLEEAGHDLKAIAANLIQSFLRHTLRDGFFHADMHPGNLFVEA
CEEEEHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEE
NGTIVAVDLGIAGRLGKKERRFLAEILYGFITRDYLRVAEVHFEAGYVPRQHNVAAFAQA
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRAIGEPIHGQPAETISMAKLLTLLFEVTELFDMATRPELILLQKTMVVVEGVARTLDPA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
FNMWKTAEPVVGGWIAGNLGPRIMMIDARDGAKALLTLARQAPELAARTDRLSREIDLMA
HHHHHCCCCHHCCEEECCCCCEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
EHGLRFDETTARAIGKAEARYSRSGRVALWVIALTLVYIAWKIL
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSVGAGFRLARAGWVLVREGVIAALPGEELSGLPKFGWQLARLFTRRRALAYQRSDRLA
CCCCCCHHHHHHCCCEEECCEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAVVRLGPSYVKLGQFLATRPDVVGNDMALDLAMLQDKMHTFPKAEAVAAIEASLGRKVR
HHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
DLYAEFGDPVAAASIAQVHAAEAIHDGVATKVAVKVIRPGVRRRFFQDLESYFLAARLQE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYIPSSRRLRPVEVTETLAQTTKIEMDLRLEAAALSELGENTKDDPGFRVPSVDWERTGR
HHCCCCCCCCCHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
DVLTMEWVDGVKMNNIAGLEEAGHDLKAIAANLIQSFLRHTLRDGFFHADMHPGNLFVEA
CEEEEHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEE
NGTIVAVDLGIAGRLGKKERRFLAEILYGFITRDYLRVAEVHFEAGYVPRQHNVAAFAQA
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRAIGEPIHGQPAETISMAKLLTLLFEVTELFDMATRPELILLQKTMVVVEGVARTLDPA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
FNMWKTAEPVVGGWIAGNLGPRIMMIDARDGAKALLTLARQAPELAARTDRLSREIDLMA
HHHHHCCCCHHCCEEECCCCCEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
EHGLRFDETTARAIGKAEARYSRSGRVALWVIALTLVYIAWKIL
HCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA