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The map label for this gene is ubiE

Identifier: 13472762

GI number: 13472762

Start: 2534782

End: 2535558

Strand: Direct

Name: ubiE

Synonym: mlr3165

Alternate gene names: 13472762

Gene position: 2534782-2535558 (Clockwise)

Preceding gene: 13472758

Following gene: 13472763

Centisome position: 36.03

GC content: 61.39

Gene sequence:

>777_bases
ATGTCAGTTGAGAGAACCACGGCCGCGGGCGGCATGGAAACCTCCTATGGTTTCAAGCGTGTGGGGGAAGGCGAGAAGCA
GTCCCTGGTCAACGATGTTTTCCACAAGGTCGCCAACCGCTACGACCTGATGAACGACCTGATGTCGGCCGGCCTGCATC
GGCTGTGGAAGGATGCCATGGTCACCTGGCTCAACCCGCCGAAGCGGCCGGGCTGGAAGGTCCTGGACGTGGCCGGCGGC
ACCGGCGATATCGCCTTCCGCATCGTCGACGCCAGCCACGGCAATGCGCATGCCACGGTGCTCGACATCAACGGCTCGAT
GCTGGGTGTCGGCCGCGATCGGGCGCAAAAGAAGGGCCTGTCCGGCAACACCGATTTCGTCGAGGCCAATGCCGAGGAAC
TGCCCTTTGCCGATGCTACCTTCGACGCCTACACCATCGCTTTCGGCATCCGCAACGTGCCACGCATCGATGTCGCGCTC
AGCGAGGCGTTTCGCGTGCTGAAGCCGGGTGGCCGGTTCCTGTGCCTGGAATTTTCCGAGGTCGAGATGCCGCTGCTCGA
CAAGGCCTATGAAGCCTGGTCGTTCAACGCCATCCCCAAGATCGGCAAGATGGTCACCGGCGACGGCGAGCCCTATTCCT
ATCTGGTTGAATCGATCGCGAAATTCCCCAACCAGCAGAATTTCGCGGCGATGATTTCCCGCGCCGGTTTCGACCGCGTT
TCCTTCCGCAATTATTCCGGCGGTATTGCCGCGCTTCATTCGGGCTGGAAGCTTTGA

Upstream 100 bases:

>100_bases
ACCCACCACCTGGAAGCGCGGCTGAACACCGCGCCTTAAACAATAGCGCCCACAGCATCGATCGGCTATGGTCCCGCCGG
TTTTATGACGGATCAAAAAA

Downstream 100 bases:

>100_bases
GGCTGGCCCGCATTTCTCATCCCCGATATGGCTCATGCCGGTCGCGAGAACCGGCCTGGGTGCGGCCATGAGCAGCGTCG
GCGCTGGTTTCAGGCTCGCG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSVERTTAAGGMETSYGFKRVGEGEKQSLVNDVFHKVANRYDLMNDLMSAGLHRLWKDAMVTWLNPPKRPGWKVLDVAGG
TGDIAFRIVDASHGNAHATVLDINGSMLGVGRDRAQKKGLSGNTDFVEANAEELPFADATFDAYTIAFGIRNVPRIDVAL
SEAFRVLKPGGRFLCLEFSEVEMPLLDKAYEAWSFNAIPKIGKMVTGDGEPYSYLVESIAKFPNQQNFAAMISRAGFDRV
SFRNYSGGIAALHSGWKL

Sequences:

>Translated_258_residues
MSVERTTAAGGMETSYGFKRVGEGEKQSLVNDVFHKVANRYDLMNDLMSAGLHRLWKDAMVTWLNPPKRPGWKVLDVAGG
TGDIAFRIVDASHGNAHATVLDINGSMLGVGRDRAQKKGLSGNTDFVEANAEELPFADATFDAYTIAFGIRNVPRIDVAL
SEAFRVLKPGGRFLCLEFSEVEMPLLDKAYEAWSFNAIPKIGKMVTGDGEPYSYLVESIAKFPNQQNFAAMISRAGFDRV
SFRNYSGGIAALHSGWKL
>Mature_257_residues
SVERTTAAGGMETSYGFKRVGEGEKQSLVNDVFHKVANRYDLMNDLMSAGLHRLWKDAMVTWLNPPKRPGWKVLDVAGGT
GDIAFRIVDASHGNAHATVLDINGSMLGVGRDRAQKKGLSGNTDFVEANAEELPFADATFDAYTIAFGIRNVPRIDVALS
EAFRVLKPGGRFLCLEFSEVEMPLLDKAYEAWSFNAIPKIGKMVTGDGEPYSYLVESIAKFPNQQNFAAMISRAGFDRVS
FRNYSGGIAALHSGWKL

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=279, Percent_Identity=47.3118279569892, Blast_Score=253, Evalue=1e-67,
Organism=Escherichia coli, GI48994983, Length=245, Percent_Identity=46.9387755102041, Blast_Score=233, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17557039, Length=249, Percent_Identity=49.3975903614458, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323526, Length=258, Percent_Identity=45.7364341085271, Blast_Score=220, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24641702, Length=260, Percent_Identity=48.8461538461538, Blast_Score=257, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_RHILO (Q98GV1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_104329.1
- ProteinModelPortal:   Q98GV1
- GeneID:   1226990
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr3165
- NMPDR:   fig|266835.1.peg.2433
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28291; Mature: 28160

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVERTTAAGGMETSYGFKRVGEGEKQSLVNDVFHKVANRYDLMNDLMSAGLHRLWKDAM
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTWLNPPKRPGWKVLDVAGGTGDIAFRIVDASHGNAHATVLDINGSMLGVGRDRAQKKGL
HHHCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCHHHHHCCC
SGNTDFVEANAEELPFADATFDAYTIAFGIRNVPRIDVALSEAFRVLKPGGRFLCLEFSE
CCCCCEEECCHHHCCCCCCCCHHEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
VEMPLLDKAYEAWSFNAIPKIGKMVTGDGEPYSYLVESIAKFPNQQNFAAMISRAGFDRV
CCCCHHHHHHHHCCCCCCHHHCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
SFRNYSGGIAALHSGWKL
EECCCCCCCHHHCCCCCC
>Mature Secondary Structure 
SVERTTAAGGMETSYGFKRVGEGEKQSLVNDVFHKVANRYDLMNDLMSAGLHRLWKDAM
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTWLNPPKRPGWKVLDVAGGTGDIAFRIVDASHGNAHATVLDINGSMLGVGRDRAQKKGL
HHHCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCHHHHHCCC
SGNTDFVEANAEELPFADATFDAYTIAFGIRNVPRIDVALSEAFRVLKPGGRFLCLEFSE
CCCCCEEECCHHHCCCCCCCCHHEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
VEMPLLDKAYEAWSFNAIPKIGKMVTGDGEPYSYLVESIAKFPNQQNFAAMISRAGFDRV
CCCCHHHHHHHHCCCCCCHHHCCEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
SFRNYSGGIAALHSGWKL
EECCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968