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The map label for this gene is afr [H]

Identifier: 13472676

GI number: 13472676

Start: 2451090

End: 2452106

Strand: Direct

Name: afr [H]

Synonym: mlr3046

Alternate gene names: 13472676

Gene position: 2451090-2452106 (Clockwise)

Preceding gene: 13472675

Following gene: 13472677

Centisome position: 34.84

GC content: 65.0

Gene sequence:

>1017_bases
GTGAGTGTCAAATGGGGGCTGATCGGCGCCAGCACGATCGCCAGGCAATTCATGATCAACGCCATTCGCGCGCAAGAGGG
GGGCGAGATCGCGGCGGTGATGAGTTCCAATCCGGAACGCGCCACGGCCTATGCCGGGGAAAACGGCATTCCGCTCGCTG
TATCCTCTCTAGATGCCTTGCTGAACTCCGACATCGACGCCGTCTACATCTCGACCACCAACGAACTGCATCTCGAACAG
GCGCTGGCCGCCATAGAGGCGGGAAAGCATGTGCTGTGCGAGAAGCCGCTGGCGCTGACCAGCGCCGACGCCCGCAAGAT
GGTCTCGGCAGCCAGGGCGGCCGGCATCGTGCTCGGCACCAATCATCATCTGCGCAACGCCGGCCCGCATCGCGCCATGC
GTGACGCCATCGCAACGGGGCACATCGGCAAGCCGATCGCCGCGCGCGTCTTCCATGCCGTCTATCTGCCGGAGACCCTG
CAGGGCTGGCGCATCACCAAACCGGAAGCGGGTGGCGGCGTGGTGCTCGACATCACCGTGCACGATGCCGACACGCTGCG
CTTCGTGCTCAGCGACGATCCCGTCGAGGTTTCGGCCTTCACGCAATCGGCCGGCATGGCCGGCGGCGGGCTGGAAGACG
GCGCCATGTGCATCTGGCGCTTCAAGTCGGGTCTCATCGCCCAGTCGCATGAAGGCTTCACCACCAAATTCGCCGGCACC
GGCTTCGAGGTGCATGGTTCGGAAGGCTCGCTGATCGCCAGCGATGTGATGACCCAGAAACCGGACGGCTCGGTGCTGCT
GCGTACGGCCAAGGGCGAGGAGCAGCTGAGCTTCGACCGCGAGGATCTCTACGTCAGATCGGTGCGTCAGTTCCATGCCG
CGATCCTCGGCAAGGGCGAGGCCGCCGCCACCGGCGAGGACGGCATCTGGTCGCTGGCATCGGCCGAGGCGGCATTGCAA
TCGGCCAGTTCCGGCAAGGCCGTCAAGATCGACCCGAAACTCGGGAGCATGAAGTGA

Upstream 100 bases:

>100_bases
GGGCCGAAGACCATATCGGCGCGGTCAATCTCGTGGTCAGGGAAAGCTGCGGTCCGGATCTCCGCACGCGAAACAGGCTG
GCGGGAACGGGAGGCGCCGC

Downstream 100 bases:

>100_bases
GCAAGGTCGTTTCAGCGGCACAGGCAGCCGGCCTGATCAAGGACGGCATGACGGTATCTGTCTCGTCGTCGAGCGGCCTT
GGCTGTCCGGACGCGGTGCT

Product: glucose-fructose oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSVKWGLIGASTIARQFMINAIRAQEGGEIAAVMSSNPERATAYAGENGIPLAVSSLDALLNSDIDAVYISTTNELHLEQ
ALAAIEAGKHVLCEKPLALTSADARKMVSAARAAGIVLGTNHHLRNAGPHRAMRDAIATGHIGKPIAARVFHAVYLPETL
QGWRITKPEAGGGVVLDITVHDADTLRFVLSDDPVEVSAFTQSAGMAGGGLEDGAMCIWRFKSGLIAQSHEGFTTKFAGT
GFEVHGSEGSLIASDVMTQKPDGSVLLRTAKGEEQLSFDREDLYVRSVRQFHAAILGKGEAAATGEDGIWSLASAEAALQ
SASSGKAVKIDPKLGSMK

Sequences:

>Translated_338_residues
MSVKWGLIGASTIARQFMINAIRAQEGGEIAAVMSSNPERATAYAGENGIPLAVSSLDALLNSDIDAVYISTTNELHLEQ
ALAAIEAGKHVLCEKPLALTSADARKMVSAARAAGIVLGTNHHLRNAGPHRAMRDAIATGHIGKPIAARVFHAVYLPETL
QGWRITKPEAGGGVVLDITVHDADTLRFVLSDDPVEVSAFTQSAGMAGGGLEDGAMCIWRFKSGLIAQSHEGFTTKFAGT
GFEVHGSEGSLIASDVMTQKPDGSVLLRTAKGEEQLSFDREDLYVRSVRQFHAAILGKGEAAATGEDGIWSLASAEAALQ
SASSGKAVKIDPKLGSMK
>Mature_337_residues
SVKWGLIGASTIARQFMINAIRAQEGGEIAAVMSSNPERATAYAGENGIPLAVSSLDALLNSDIDAVYISTTNELHLEQA
LAAIEAGKHVLCEKPLALTSADARKMVSAARAAGIVLGTNHHLRNAGPHRAMRDAIATGHIGKPIAARVFHAVYLPETLQ
GWRITKPEAGGGVVLDITVHDADTLRFVLSDDPVEVSAFTQSAGMAGGGLEDGAMCIWRFKSGLIAQSHEGFTTKFAGTG
FEVHGSEGSLIASDVMTQKPDGSVLLRTAKGEEQLSFDREDLYVRSVRQFHAAILGKGEAAATGEDGIWSLASAEAALQS
ASSGKAVKIDPKLGSMK

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=184, Percent_Identity=27.7173913043478, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787574, Length=348, Percent_Identity=27.2988505747126, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082405, Length=241, Percent_Identity=28.6307053941909, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081947, Length=187, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693182, Length=137, Percent_Identity=32.8467153284672, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581117, Length=255, Percent_Identity=26.6666666666667, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24581115, Length=199, Percent_Identity=26.1306532663317, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584727, Length=256, Percent_Identity=25.390625, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35457; Mature: 35325

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKWGLIGASTIARQFMINAIRAQEGGEIAAVMSSNPERATAYAGENGIPLAVSSLDAL
CCEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHH
LNSDIDAVYISTTNELHLEQALAAIEAGKHVLCEKPLALTSADARKMVSAARAAGIVLGT
HCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCEECCHHHHHHHHHHHHCEEEEEC
NHHLRNAGPHRAMRDAIATGHIGKPIAARVFHAVYLPETLQGWRITKPEAGGGVVLDITV
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEE
HDADTLRFVLSDDPVEVSAFTQSAGMAGGGLEDGAMCIWRFKSGLIAQSHEGFTTKFAGT
ECCCEEEEEECCCCEEEEEEHHHCCCCCCCCCCCCEEEEEECCCCEEECCCCCEEEECCC
GFEVHGSEGSLIASDVMTQKPDGSVLLRTAKGEEQLSFDREDLYVRSVRQFHAAILGKGE
CEEEECCCCCEEEHHHHCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCC
AAATGEDGIWSLASAEAALQSASSGKAVKIDPKLGSMK
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
SVKWGLIGASTIARQFMINAIRAQEGGEIAAVMSSNPERATAYAGENGIPLAVSSLDAL
CEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHH
LNSDIDAVYISTTNELHLEQALAAIEAGKHVLCEKPLALTSADARKMVSAARAAGIVLGT
HCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCEECCHHHHHHHHHHHHCEEEEEC
NHHLRNAGPHRAMRDAIATGHIGKPIAARVFHAVYLPETLQGWRITKPEAGGGVVLDITV
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEEE
HDADTLRFVLSDDPVEVSAFTQSAGMAGGGLEDGAMCIWRFKSGLIAQSHEGFTTKFAGT
ECCCEEEEEECCCCEEEEEEHHHCCCCCCCCCCCCEEEEEECCCCEEECCCCCEEEECCC
GFEVHGSEGSLIASDVMTQKPDGSVLLRTAKGEEQLSFDREDLYVRSVRQFHAAILGKGE
CEEEECCCCCEEEHHHHCCCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCC
AAATGEDGIWSLASAEAALQSASSGKAVKIDPKLGSMK
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA