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The map label for this gene is malI [H]

Identifier: 13472675

GI number: 13472675

Start: 2450035

End: 2451093

Strand: Direct

Name: malI [H]

Synonym: mlr3045

Alternate gene names: 13472675

Gene position: 2450035-2451093 (Clockwise)

Preceding gene: 13472672

Following gene: 13472676

Centisome position: 34.82

GC content: 65.91

Gene sequence:

>1059_bases
ATGGCCAGCCGGGCCAAGGCCACGATATTCGACATTGCCCGTGAGGCGGGCGTGTCCAAATCTACGGTATCGCTCGTGCT
CCAGGGCAGCGGGCTGATCCGGCCTGAAACCGCGGTCAAGGTGCGCAAGGCGATCGAGGATGTCGGCTATGTCTACAACC
GCGGCGCCGCCAATCTGCGCAAGGCCCATTCCAATGTCATCGGCATGGTCATCAACGACCTCACCAACCCGTTCTTCGCC
GAGCTGGCGGTCGGCATGGAACGCGTCTTCCAGTCGGCCGGCATCGTGCCCTTCATCGCCAATACGGCGGAGAATCCGGT
GCGCCAGGAAGAGGTGCTGAAGTCGCTGATGGAACAAGGCGTCGCCGGCCTCATCGTGTCACCGGCGCGCGGCACCACGC
CAGGCGCTTTCCGGCGGCTGGAGTTGGCGGGCGTGCCGGTGGTCTTCGCCATGCGCCGCCTGCCTGAAAGCCGCATCCCT
GTGATCGCGCCCGACAATCATCGCGGCGCCTATCTTGCCGCGGCTCATCTGATCGCCAAAGGACATCGCCGGCTCGCCTT
CTTCGGCGGCACCTCGGATCTGGTGGTCTATCAGGAACGGCTGGGCGGCTTTCGCGAAGCCTGCGAGACGTTGGGCATCG
CTGCCCGCGATGTGCTGGTCGTCGAGGGCGAAACCAACCGCAGAGGCGGCCTCGCTTGCCTGGAAACCGCCCTTACAATG
GCTGAGCCTCCGACCGCGGCCCTCTGCTTCAACGATGCCGTCGCGTTTGGCGTGATGCTGGCGTTGCGCAAGCGTCGGCT
TGAGCCGGGACCGGATTTCGCCGTCGTCGGCTTCGACGATGTGGTCGAGGCCGAACACTACATGCCGGCGCTGACCAGCG
TCGCGGTGGATACAGCCGGCCTGGGCGAGCGCGCCGCCCACGTCATGCTCAAGCTGATCCAGTCGCGCACCACGCGGGCC
GAAGACCATATCGGCGCGGTCAATCTCGTGGTCAGGGAAAGCTGCGGTCCGGATCTCCGCACGCGAAACAGGCTGGCGGG
AACGGGAGGCGCCGCGTGA

Upstream 100 bases:

>100_bases
AGGTTTGCGGACTGAGAAAAATTCGCGGTTGACTCAGGATTGAACCAAGGATTAGAACGTTCCAATAGATTGGTTGGGAA
AACTGGGAGGACGCATCGAT

Downstream 100 bases:

>100_bases
GTGTCAAATGGGGGCTGATCGGCGCCAGCACGATCGCCAGGCAATTCATGATCAACGCCATTCGCGCGCAAGAGGGGGGC
GAGATCGCGGCGGTGATGAG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MASRAKATIFDIAREAGVSKSTVSLVLQGSGLIRPETAVKVRKAIEDVGYVYNRGAANLRKAHSNVIGMVINDLTNPFFA
ELAVGMERVFQSAGIVPFIANTAENPVRQEEVLKSLMEQGVAGLIVSPARGTTPGAFRRLELAGVPVVFAMRRLPESRIP
VIAPDNHRGAYLAAAHLIAKGHRRLAFFGGTSDLVVYQERLGGFREACETLGIAARDVLVVEGETNRRGGLACLETALTM
AEPPTAALCFNDAVAFGVMLALRKRRLEPGPDFAVVGFDDVVEAEHYMPALTSVAVDTAGLGERAAHVMLKLIQSRTTRA
EDHIGAVNLVVRESCGPDLRTRNRLAGTGGAA

Sequences:

>Translated_352_residues
MASRAKATIFDIAREAGVSKSTVSLVLQGSGLIRPETAVKVRKAIEDVGYVYNRGAANLRKAHSNVIGMVINDLTNPFFA
ELAVGMERVFQSAGIVPFIANTAENPVRQEEVLKSLMEQGVAGLIVSPARGTTPGAFRRLELAGVPVVFAMRRLPESRIP
VIAPDNHRGAYLAAAHLIAKGHRRLAFFGGTSDLVVYQERLGGFREACETLGIAARDVLVVEGETNRRGGLACLETALTM
AEPPTAALCFNDAVAFGVMLALRKRRLEPGPDFAVVGFDDVVEAEHYMPALTSVAVDTAGLGERAAHVMLKLIQSRTTRA
EDHIGAVNLVVRESCGPDLRTRNRLAGTGGAA
>Mature_351_residues
ASRAKATIFDIAREAGVSKSTVSLVLQGSGLIRPETAVKVRKAIEDVGYVYNRGAANLRKAHSNVIGMVINDLTNPFFAE
LAVGMERVFQSAGIVPFIANTAENPVRQEEVLKSLMEQGVAGLIVSPARGTTPGAFRRLELAGVPVVFAMRRLPESRIPV
IAPDNHRGAYLAAAHLIAKGHRRLAFFGGTSDLVVYQERLGGFREACETLGIAARDVLVVEGETNRRGGLACLETALTMA
EPPTAALCFNDAVAFGVMLALRKRRLEPGPDFAVVGFDDVVEAEHYMPALTSVAVDTAGLGERAAHVMLKLIQSRTTRAE
DHIGAVNLVVRESCGPDLRTRNRLAGTGGAA

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=293, Percent_Identity=34.1296928327645, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=31.8339100346021, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=32.0634920634921, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=24.6951219512195, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789068, Length=291, Percent_Identity=29.2096219931272, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787948, Length=311, Percent_Identity=27.6527331189711, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=27.4143302180685, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788474, Length=309, Percent_Identity=28.1553398058252, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=26.8436578171091, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=25.3205128205128, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=26.8987341772152, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786268, Length=304, Percent_Identity=25.6578947368421, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790192, Length=232, Percent_Identity=26.7241379310345, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37563; Mature: 37432

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRAKATIFDIAREAGVSKSTVSLVLQGSGLIRPETAVKVRKAIEDVGYVYNRGAANLR
CCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
KAHSNVIGMVINDLTNPFFAELAVGMERVFQSAGIVPFIANTAENPVRQEEVLKSLMEQG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCC
VAGLIVSPARGTTPGAFRRLELAGVPVVFAMRRLPESRIPVIAPDNHRGAYLAAAHLIAK
CCEEEEECCCCCCCHHHHEEHHCCCHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHC
GHRRLAFFGGTSDLVVYQERLGGFREACETLGIAARDVLVVEGETNRRGGLACLETALTM
CCCEEEEECCCCCHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
AEPPTAALCFNDAVAFGVMLALRKRRLEPGPDFAVVGFDDVVEAEHYMPALTSVAVDTAG
CCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCC
LGERAAHVMLKLIQSRTTRAEDHIGAVNLVVRESCGPDLRTRNRLAGTGGAA
CHHHHHHHHHHHHHHHCCCHHHHCCHHHEEEECCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
ASRAKATIFDIAREAGVSKSTVSLVLQGSGLIRPETAVKVRKAIEDVGYVYNRGAANLR
CCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
KAHSNVIGMVINDLTNPFFAELAVGMERVFQSAGIVPFIANTAENPVRQEEVLKSLMEQG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCC
VAGLIVSPARGTTPGAFRRLELAGVPVVFAMRRLPESRIPVIAPDNHRGAYLAAAHLIAK
CCEEEEECCCCCCCHHHHEEHHCCCHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHC
GHRRLAFFGGTSDLVVYQERLGGFREACETLGIAARDVLVVEGETNRRGGLACLETALTM
CCCEEEEECCCCCHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
AEPPTAALCFNDAVAFGVMLALRKRRLEPGPDFAVVGFDDVVEAEHYMPALTSVAVDTAG
CCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCC
LGERAAHVMLKLIQSRTTRAEDHIGAVNLVVRESCGPDLRTRNRLAGTGGAA
CHHHHHHHHHHHHHHHCCCHHHHCCHHHEEEECCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]