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The map label for this gene is yfkN [H]

Identifier: 13472651

GI number: 13472651

Start: 2420087

End: 2422207

Strand: Direct

Name: yfkN [H]

Synonym: mlr3017

Alternate gene names: 13472651

Gene position: 2420087-2422207 (Clockwise)

Preceding gene: 13472650

Following gene: 13472652

Centisome position: 34.4

GC content: 64.07

Gene sequence:

>2121_bases
ATGAAGAAGCTTGCCGCCATTGCCGCCCTGTCCGTATCGACGCTTGGCCTGTCGGCCGGCGCATCCTTTGCCGACTACAC
ATTGAACATTCTGCATTTCAATGACTGGCACAGCCGCATCGAAGGCAACAACAAATACGAGTCGACCTGTTCGGCCGACG
AGGAGACCAAGGGCGAATGCATCGGCGGCGCCGGCCGGTTGATCACGGCGATCGCCCAGGAACGCAAGAAGCTCGAAGGC
CAGAACGTGCTGCTGCTCAATGCAGGCGACAGCTTCCAGGGATCGCTGTTCTACACCACCTATAAAGGCACCGTGGAGGA
GGAATTCCTCAATCAGATGAAACCCGACGCGGTGACGCTCGGCAACCATGAATTCGACGACGGCGAGAGCGCCCTGGTGC
CTTACCTCGACAAGGCGAAATTCCCGATCGTCAGTGCCAATGTCATGCCCAACGACAAGTCCGGCGCGGCGGGCAAGATC
AAACCGTCGATCGTCGTGGAAGTCGGCGGCCAGAAGATCGGCATTGTCGGCGCCGTCACCAACGACACGCCCGAACTCGC
CTCCCCCGGCCCCAACATCGCGATCGCGGACGACGTCGAGTCGATCACCGCGGAAGTCGAGAAGCTCAAGGCGCAAGGGG
TCAACAAGATCATCGCGGTGACCCATATCGGCTACAACAGGGAACGCGACATCATCGCCAAGATCCCGGGCGTCGACGTG
GTCGTCGGCGGCCACAGCCATACGCTCCTGTCGAACACCGATCCGAAGGCGGCGGGCCCCTATCCGACGATGGTCGACAA
CCCCGGCGGCTACAAGGTGCCGGTGGTGCAGGCGGCCTCCTATTCGAAATATCTCGGCGAGTTCAAGGTGGTGTTCGACG
ACAACGGCGTCGTCAAGTCGGCAAGCGGCGATCCGATCTTTCTCGACAAGTCGATCACGCCCGATCCCGCCGTGCTCGCC
CGCATCAAGGAACTCGGCGCGCCGATCGAGGCGCTGAAGAACAAGGAAGTGGCCGAGACCACCGATGTCATCGACGGCAG
CCGCGAGAATTGCCGCGCCAAGGAATGCGAGATGGGCAACCTCGTCTCCGACGCCATCCTCGACCGCGTCAAGGGACAGG
GCGTCGAGATCGTCATCTCCAATGGCGGCGGCCTGCGCGCCTCGATCGACAAGGGCACCGTCACCATGGGTGAGGTGCTG
ACCGTGCTGCCGTTCCAGAACACGCTGGCGACCTTCCAGATTTCCGGCAAGGACCTGGTCGCCGGGCTGGAAAGCGGCCT
CAGCCAGATCGAGGACGGCGCCGGCCGCTTCCCGCAGGTGGCGGGCCTCAAATATTCCTTCGACAAGTCGGTCGCGCCCA
ATGCCGGCCGCGTCAAATCCGTCGAGGTCATGGAGAACGGCGCCTGGACGCCGATCAAGCCAGACAAGGACTATCTGGTC
GCCACCAACAATTACGTCCGTCAGGGCGGCGACGGCTACAAGGTCTTCGCCGAAAGGGCCAAGAACGCCTACGACTACGG
CCCCGGTCTCGAACAGGTCGTTGCCGACTATCTCGGCGCCCACCGGCCCTACACGCCCAAGCTCGACGGCCGCATCACCG
AGATCGCCGCGACCGTCGCGGCGGCCCCGGCAGCGGAACCGGCAAAGCCAGCCGAGGCAACCCCAGCCCCCGCGCCGGCG
CAACCTGCCAAACCGGCCGAGGCAGCGAAACCGGCTGAACCAGCAAAGCCCGCTGAAGCGGCACCGGCAATGCCCGAATT
GCCCGCCAACTCGGGCAACATCGCCAACACGCCGCCAGCCATATCGACGGAGGCGGCACCTCCGCCAGCTCCCGCCACTC
CAGCACCTGCGCCAGCCGAACCGGCGAAACCAGCTGAGGCTGCGCCCGCACCAGCGGCACCGGTGCCAGCGGAACCTGCC
AAGCCGGCCGAACCGGCAAAGCCCGCTGAAGCCGCACCCGCCACGGGCAGCCATGTCATCGTTGCCGGCGACACCTATTG
GGACCTGGCCAAGAAGGCCTATGGCGACGCCACCAAGTGGAAACTCATCTACGAGGCCAACAAGGGTCAAAGGCCACATC
GGCTTACCCCTGGCGCAACGTTGACCATCCCGGCGAAGTAA

Upstream 100 bases:

>100_bases
TCAGCCGGATCGCGATGGGAACATGCTCAAAACGCTGGCTGTTATATCCCAGTCAAGCAAGCGCGCTAAACGCCTCTGAT
CAGCCTACCGGGAGACGAAG

Downstream 100 bases:

>100_bases
CGCGCCTCGCATCCTTCAACGACGAGCCCGCTCGGGAAACCGGGCGGGTTTTGCGTTTTCGGGCTAAGGTGCGGCCAGAC
GCGGCATTGAAAACCGGCGC

Product: 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MKKLAAIAALSVSTLGLSAGASFADYTLNILHFNDWHSRIEGNNKYESTCSADEETKGECIGGAGRLITAIAQERKKLEG
QNVLLLNAGDSFQGSLFYTTYKGTVEEEFLNQMKPDAVTLGNHEFDDGESALVPYLDKAKFPIVSANVMPNDKSGAAGKI
KPSIVVEVGGQKIGIVGAVTNDTPELASPGPNIAIADDVESITAEVEKLKAQGVNKIIAVTHIGYNRERDIIAKIPGVDV
VVGGHSHTLLSNTDPKAAGPYPTMVDNPGGYKVPVVQAASYSKYLGEFKVVFDDNGVVKSASGDPIFLDKSITPDPAVLA
RIKELGAPIEALKNKEVAETTDVIDGSRENCRAKECEMGNLVSDAILDRVKGQGVEIVISNGGGLRASIDKGTVTMGEVL
TVLPFQNTLATFQISGKDLVAGLESGLSQIEDGAGRFPQVAGLKYSFDKSVAPNAGRVKSVEVMENGAWTPIKPDKDYLV
ATNNYVRQGGDGYKVFAERAKNAYDYGPGLEQVVADYLGAHRPYTPKLDGRITEIAATVAAAPAAEPAKPAEATPAPAPA
QPAKPAEAAKPAEPAKPAEAAPAMPELPANSGNIANTPPAISTEAAPPPAPATPAPAPAEPAKPAEAAPAPAAPVPAEPA
KPAEPAKPAEAAPATGSHVIVAGDTYWDLAKKAYGDATKWKLIYEANKGQRPHRLTPGATLTIPAK

Sequences:

>Translated_706_residues
MKKLAAIAALSVSTLGLSAGASFADYTLNILHFNDWHSRIEGNNKYESTCSADEETKGECIGGAGRLITAIAQERKKLEG
QNVLLLNAGDSFQGSLFYTTYKGTVEEEFLNQMKPDAVTLGNHEFDDGESALVPYLDKAKFPIVSANVMPNDKSGAAGKI
KPSIVVEVGGQKIGIVGAVTNDTPELASPGPNIAIADDVESITAEVEKLKAQGVNKIIAVTHIGYNRERDIIAKIPGVDV
VVGGHSHTLLSNTDPKAAGPYPTMVDNPGGYKVPVVQAASYSKYLGEFKVVFDDNGVVKSASGDPIFLDKSITPDPAVLA
RIKELGAPIEALKNKEVAETTDVIDGSRENCRAKECEMGNLVSDAILDRVKGQGVEIVISNGGGLRASIDKGTVTMGEVL
TVLPFQNTLATFQISGKDLVAGLESGLSQIEDGAGRFPQVAGLKYSFDKSVAPNAGRVKSVEVMENGAWTPIKPDKDYLV
ATNNYVRQGGDGYKVFAERAKNAYDYGPGLEQVVADYLGAHRPYTPKLDGRITEIAATVAAAPAAEPAKPAEATPAPAPA
QPAKPAEAAKPAEPAKPAEAAPAMPELPANSGNIANTPPAISTEAAPPPAPATPAPAPAEPAKPAEAAPAPAAPVPAEPA
KPAEPAKPAEAAPATGSHVIVAGDTYWDLAKKAYGDATKWKLIYEANKGQRPHRLTPGATLTIPAK
>Mature_706_residues
MKKLAAIAALSVSTLGLSAGASFADYTLNILHFNDWHSRIEGNNKYESTCSADEETKGECIGGAGRLITAIAQERKKLEG
QNVLLLNAGDSFQGSLFYTTYKGTVEEEFLNQMKPDAVTLGNHEFDDGESALVPYLDKAKFPIVSANVMPNDKSGAAGKI
KPSIVVEVGGQKIGIVGAVTNDTPELASPGPNIAIADDVESITAEVEKLKAQGVNKIIAVTHIGYNRERDIIAKIPGVDV
VVGGHSHTLLSNTDPKAAGPYPTMVDNPGGYKVPVVQAASYSKYLGEFKVVFDDNGVVKSASGDPIFLDKSITPDPAVLA
RIKELGAPIEALKNKEVAETTDVIDGSRENCRAKECEMGNLVSDAILDRVKGQGVEIVISNGGGLRASIDKGTVTMGEVL
TVLPFQNTLATFQISGKDLVAGLESGLSQIEDGAGRFPQVAGLKYSFDKSVAPNAGRVKSVEVMENGAWTPIKPDKDYLV
ATNNYVRQGGDGYKVFAERAKNAYDYGPGLEQVVADYLGAHRPYTPKLDGRITEIAATVAAAPAAEPAKPAEATPAPAPA
QPAKPAEAAKPAEPAKPAEAAPAMPELPANSGNIANTPPAISTEAAPPPAPATPAPAPAEPAKPAEAAPAPAAPVPAEPA
KPAEPAKPAEAAPATGSHVIVAGDTYWDLAKKAYGDATKWKLIYEANKGQRPHRLTPGATLTIPAK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=550, Percent_Identity=38, Blast_Score=331, Evalue=2e-90,
Organism=Escherichia coli, GI1786687, Length=536, Percent_Identity=26.3059701492537, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1790658, Length=280, Percent_Identity=26.4285714285714, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922444, Length=534, Percent_Identity=35.9550561797753, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI19922446, Length=535, Percent_Identity=34.9532710280374, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24654424, Length=535, Percent_Identity=34.9532710280374, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24641187, Length=533, Percent_Identity=33.5834896810507, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI28573524, Length=530, Percent_Identity=33.7735849056604, Blast_Score=287, Evalue=2e-77,
Organism=Drosophila melanogaster, GI221329836, Length=527, Percent_Identity=31.8785578747628, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI19921980, Length=480, Percent_Identity=26.875, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI161076508, Length=480, Percent_Identity=26.875, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24652512, Length=480, Percent_Identity=26.875, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 73627; Mature: 73627

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLAAIAALSVSTLGLSAGASFADYTLNILHFNDWHSRIEGNNKYESTCSADEETKGEC
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCE
IGGAGRLITAIAQERKKLEGQNVLLLNAGDSFQGSLFYTTYKGTVEEEFLNQMKPDAVTL
ECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEE
GNHEFDDGESALVPYLDKAKFPIVSANVMPNDKSGAAGKIKPSIVVEVGGQKIGIVGAVT
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEC
NDTPELASPGPNIAIADDVESITAEVEKLKAQGVNKIIAVTHIGYNRERDIIAKIPGVDV
CCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCEE
VVGGHSHTLLSNTDPKAAGPYPTMVDNPGGYKVPVVQAASYSKYLGEFKVVFDDNGVVKS
EECCCCCEEECCCCCCCCCCCCCEEECCCCEEEEEEECCHHHHHCCCEEEEECCCCEEEC
ASGDPIFLDKSITPDPAVLARIKELGAPIEALKNKEVAETTDVIDGSRENCRAKECEMGN
CCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCC
LVSDAILDRVKGQGVEIVISNGGGLRASIDKGTVTMGEVLTVLPFQNTLATFQISGKDLV
HHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEHHHHEEEEECCCCEEEEEECCHHHH
AGLESGLSQIEDGAGRFPQVAGLKYSFDKSVAPNAGRVKSVEVMENGAWTPIKPDKDYLV
HHHHHHHHHHHHCCCCCCHHCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEE
ATNNYVRQGGDGYKVFAERAKNAYDYGPGLEQVVADYLGAHRPYTPKLDGRITEIAATVA
EECCHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AAPAAEPAKPAEATPAPAPAQPAKPAEAAKPAEPAKPAEAAPAMPELPANSGNIANTPPA
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ISTEAAPPPAPATPAPAPAEPAKPAEAAPAPAAPVPAEPAKPAEPAKPAEAAPATGSHVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VAGDTYWDLAKKAYGDATKWKLIYEANKGQRPHRLTPGATLTIPAK
EECCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCCCEEEECCC
>Mature Secondary Structure
MKKLAAIAALSVSTLGLSAGASFADYTLNILHFNDWHSRIEGNNKYESTCSADEETKGEC
CCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCE
IGGAGRLITAIAQERKKLEGQNVLLLNAGDSFQGSLFYTTYKGTVEEEFLNQMKPDAVTL
ECCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEE
GNHEFDDGESALVPYLDKAKFPIVSANVMPNDKSGAAGKIKPSIVVEVGGQKIGIVGAVT
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEC
NDTPELASPGPNIAIADDVESITAEVEKLKAQGVNKIIAVTHIGYNRERDIIAKIPGVDV
CCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCEE
VVGGHSHTLLSNTDPKAAGPYPTMVDNPGGYKVPVVQAASYSKYLGEFKVVFDDNGVVKS
EECCCCCEEECCCCCCCCCCCCCEEECCCCEEEEEEECCHHHHHCCCEEEEECCCCEEEC
ASGDPIFLDKSITPDPAVLARIKELGAPIEALKNKEVAETTDVIDGSRENCRAKECEMGN
CCCCEEEECCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCC
LVSDAILDRVKGQGVEIVISNGGGLRASIDKGTVTMGEVLTVLPFQNTLATFQISGKDLV
HHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEHHHHEEEEECCCCEEEEEECCHHHH
AGLESGLSQIEDGAGRFPQVAGLKYSFDKSVAPNAGRVKSVEVMENGAWTPIKPDKDYLV
HHHHHHHHHHHHCCCCCCHHCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEE
ATNNYVRQGGDGYKVFAERAKNAYDYGPGLEQVVADYLGAHRPYTPKLDGRITEIAATVA
EECCHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AAPAAEPAKPAEATPAPAPAQPAKPAEAAKPAEPAKPAEAAPAMPELPANSGNIANTPPA
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ISTEAAPPPAPATPAPAPAEPAKPAEAAPAPAAPVPAEPAKPAEPAKPAEAAPATGSHVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VAGDTYWDLAKKAYGDATKWKLIYEANKGQRPHRLTPGATLTIPAK
EECCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]