Click here to switch to the map view.

The map label for this gene is potH [H]

Identifier: 13472604

GI number: 13472604

Start: 2373134

End: 2374018

Strand: Reverse

Name: potH [H]

Synonym: mll2950

Alternate gene names: 13472604

Gene position: 2374018-2373134 (Counterclockwise)

Preceding gene: 13472605

Following gene: 13472603

Centisome position: 33.74

GC content: 66.55

Gene sequence:

>885_bases
GTGAGCGCGTCCGACCCGACAAACGGATCCGTTGCCCAACCCGCCGCCGAAGGGCGGCCGGGGTGGGCGTGGCGGCCGTT
CGCGCTTGCGCTGCCGGCGCTGGTGCTGCTCGTCGCGGTGATCGGCTATCCGCTGTTCACCATCGTGCTGCGCAGCCTGT
CGGAGCCGCAATGGGGCTTGCAGAACTATGCCTGGTTCTTCGGCGCATCGGTCAACCTCACCGTGCTTCAGCGGACCTTC
GCCATCTCGGCCTGGGTCACGCTGGTGTGCGTGATCGCGGCCTACCCCTACGCCTATCTGATGACCGCGGTCGGGCCGCG
CATCCGGCTGGCGCTGGTGTTATGCGTGCTGATTCCGTTCTGGGTGAGCGGGGTGGTTCGCACGCTCTCCTGGGTGATCC
TGCTGCAGGATTCGGGCGTCATCAATTCGCTGCTGCGCGCAGCCGGGTTCGACGGCATCAAGTTGATCCGAACCCAGACG
GGCGTGGTGATCGGCATGGCACAGGTGCTTTTGCCGTTCATGATCCTGCCGCTCTATTCGGTCATGAAAGGCATCGACCT
CAGGCTGATGCGCGCGGCGCAAAGTCTTGGCGCACGGCCGTCGCGCGCCTTCTTCACCGTCTATCTGCCGCTGTCCTTGC
CGGGGGTCTATGCCGGCGCGATCATCGTGTTCATCCTGGCGCTGGGCTTCTACATCACCCCGGCCTTGCTCGGCGGGCCG
CGCTCGACGATGCTGTCGACGCTGGTGCAGACGCAGGTGCTCAGCCTGCTGCAATGGGGCAGGGGCGGCGCGATGGGCGT
GGTGCTGCTCGTCGCAACCTTCGTGCTCCTGGCGCTCGCCGCACCGGTGATGCGATCCAGGTACCGCGAAGCAGGGCGGC
GCTGA

Upstream 100 bases:

>100_bases
AAGCAGAACTTCAAGTTCTGGGTCCCGAACTTCGCGGTGGCTCAGGAGAAGTGGTCGGCGCTGATGGCCGGCAACTGAGC
GAGCGTCGCAGAGGCAGGCG

Downstream 100 bases:

>100_bases
TGGAGATGAAGCCGCTCACGCGCCTGCTTTTGGGCCTGGTCTGCCTGCTGGTGGCGATATGGCTGGTGGCGCCGACGCTG
GTCGTCGTGCCGATGTCGTT

Product: hypothetical protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSASDPTNGSVAQPAAEGRPGWAWRPFALALPALVLLVAVIGYPLFTIVLRSLSEPQWGLQNYAWFFGASVNLTVLQRTF
AISAWVTLVCVIAAYPYAYLMTAVGPRIRLALVLCVLIPFWVSGVVRTLSWVILLQDSGVINSLLRAAGFDGIKLIRTQT
GVVIGMAQVLLPFMILPLYSVMKGIDLRLMRAAQSLGARPSRAFFTVYLPLSLPGVYAGAIIVFILALGFYITPALLGGP
RSTMLSTLVQTQVLSLLQWGRGGAMGVVLLVATFVLLALAAPVMRSRYREAGRR

Sequences:

>Translated_294_residues
MSASDPTNGSVAQPAAEGRPGWAWRPFALALPALVLLVAVIGYPLFTIVLRSLSEPQWGLQNYAWFFGASVNLTVLQRTF
AISAWVTLVCVIAAYPYAYLMTAVGPRIRLALVLCVLIPFWVSGVVRTLSWVILLQDSGVINSLLRAAGFDGIKLIRTQT
GVVIGMAQVLLPFMILPLYSVMKGIDLRLMRAAQSLGARPSRAFFTVYLPLSLPGVYAGAIIVFILALGFYITPALLGGP
RSTMLSTLVQTQVLSLLQWGRGGAMGVVLLVATFVLLALAAPVMRSRYREAGRR
>Mature_293_residues
SASDPTNGSVAQPAAEGRPGWAWRPFALALPALVLLVAVIGYPLFTIVLRSLSEPQWGLQNYAWFFGASVNLTVLQRTFA
ISAWVTLVCVIAAYPYAYLMTAVGPRIRLALVLCVLIPFWVSGVVRTLSWVILLQDSGVINSLLRAAGFDGIKLIRTQTG
VVIGMAQVLLPFMILPLYSVMKGIDLRLMRAAQSLGARPSRAFFTVYLPLSLPGVYAGAIIVFILALGFYITPALLGGPR
STMLSTLVQTQVLSLLQWGRGGAMGVVLLVATFVLLALAAPVMRSRYREAGRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=222, Percent_Identity=32.4324324324324, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=28.6324786324786, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787713, Length=236, Percent_Identity=29.6610169491525, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31782; Mature: 31650

Theoretical pI: Translated: 11.28; Mature: 11.28

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDPTNGSVAQPAAEGRPGWAWRPFALALPALVLLVAVIGYPLFTIVLRSLSEPQWGL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QNYAWFFGASVNLTVLQRTFAISAWVTLVCVIAAYPYAYLMTAVGPRIRLALVLCVLIPF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
WVSGVVRTLSWVILLQDSGVINSLLRAAGFDGIKLIRTQTGVVIGMAQVLLPFMILPLYS
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMKGIDLRLMRAAQSLGARPSRAFFTVYLPLSLPGVYAGAIIVFILALGFYITPALLGGP
HHHCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RSTMLSTLVQTQVLSLLQWGRGGAMGVVLLVATFVLLALAAPVMRSRYREAGRR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SASDPTNGSVAQPAAEGRPGWAWRPFALALPALVLLVAVIGYPLFTIVLRSLSEPQWGL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QNYAWFFGASVNLTVLQRTFAISAWVTLVCVIAAYPYAYLMTAVGPRIRLALVLCVLIPF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
WVSGVVRTLSWVILLQDSGVINSLLRAAGFDGIKLIRTQTGVVIGMAQVLLPFMILPLYS
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMKGIDLRLMRAAQSLGARPSRAFFTVYLPLSLPGVYAGAIIVFILALGFYITPALLGGP
HHHCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RSTMLSTLVQTQVLSLLQWGRGGAMGVVLLVATFVLLALAAPVMRSRYREAGRR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]