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The map label for this gene is sbp [H]

Identifier: 13471636

GI number: 13471636

Start: 1397519

End: 1398547

Strand: Direct

Name: sbp [H]

Synonym: mlr1666

Alternate gene names: 13471636

Gene position: 1397519-1398547 (Clockwise)

Preceding gene: 13471635

Following gene: 13471637

Centisome position: 19.86

GC content: 61.61

Gene sequence:

>1029_bases
ATGACCAAACCTCATTTCAGGAAACTGCTCGGCGCACTGGTCGCCACATCTGTCCAGTTCGGCACGCTCGGTTTCGCGTT
CGCCGACACCACCATCCTCAACGTGTCCTATGATCCGACGCGTGAGCTCTACAAGGCCTATGACGAGGCATTTGCCGCCC
ACTGGAAGGCCGAGACCGGCGAGACGGTGACCATCCAGCAGTCGCATGGCGGATCGGGCGCCCAAGCCCGCGCCGTGATC
GACGGCCTCGACGCCGACGTGGTGACGCTGGCGCTCGAGGGCGACATCAACGCCATCGTCTCCAAGTCGAAGAAGATCAA
TCCCGACTGGCGCAAGAAATTCGAAAACAATTCAGCGCCTTACACCTCGACCATCATCTTCCTGGTCCGCAAGGGCAATC
CCAAGGGCATCCACGACTGGAGCGACCTGGTCAAGGACGGCACCCAGGTGATCACGCCCAATCCCAAGACCTCAGGCGGT
GCCCGCTGGAACTACCTGGCAGCCTGGGCCTACGCCAACGCCCATGATGGCGGCGACGAGGCCAAAACCAAGGAGTTCGT
CGGCAAGCTCTACGCCAATGCCCCGGTTCTCGACACCGGCGCACGCGGCTCGACCGTCACCTTCGCACAAAAGGGCATCG
GCGACGTGCTGATCGGCTGGGAAAACGACGCCTATCTGGCGCTCGACGAATTCGGCGCCGACAATTTCGAGATCGTCTAT
CCGCCGACATCGATCCTGGCCGAGCCGCCGGTGGCAGTCGTCGACGCCAACGTCGATGCCAAGGGCACGCGCAAGGTGGC
CGAAGCCTATCTCGGCTGGCTCTATTCCAAGGAAGGCCAGACGATCATCGCCAAGAACCACTATCGCCCGGCCAAGCCCG
AACTGGTGGCGGCCGTCGATCTGCCCAAGACCCCCGATATCAAGCTGATCTCCATCGACGATCCGCTTTTCGGCGGCTGG
AAGAAGGCGCAGCCTTATCATTTCGGCGACGGTGGTATATTCGACCAGATCTACAAGCCGGCCCAGTGA

Upstream 100 bases:

>100_bases
ATCGTCGAAGATTTTCTTCAGAAGGGCAAAAAAGGCAACATTCGATGCATCTAATTTCTATCAGTTCGCTAGATTTGGCG
CGGGTTCAACGGAGGCAGAA

Downstream 100 bases:

>100_bases
GGTTCGGCAGTCGCGAAGAAGCCCGCGAATGGGCTTCTTCGGCAAAGTCTGGTTGAGTAAGAAGGGACGATCCAGAACTC
CATGACCACAGCACCCGCGC

Product: sulfate binding protein of ABC transporter

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTKPHFRKLLGALVATSVQFGTLGFAFADTTILNVSYDPTRELYKAYDEAFAAHWKAETGETVTIQQSHGGSGAQARAVI
DGLDADVVTLALEGDINAIVSKSKKINPDWRKKFENNSAPYTSTIIFLVRKGNPKGIHDWSDLVKDGTQVITPNPKTSGG
ARWNYLAAWAYANAHDGGDEAKTKEFVGKLYANAPVLDTGARGSTVTFAQKGIGDVLIGWENDAYLALDEFGADNFEIVY
PPTSILAEPPVAVVDANVDAKGTRKVAEAYLGWLYSKEGQTIIAKNHYRPAKPELVAAVDLPKTPDIKLISIDDPLFGGW
KKAQPYHFGDGGIFDQIYKPAQ

Sequences:

>Translated_342_residues
MTKPHFRKLLGALVATSVQFGTLGFAFADTTILNVSYDPTRELYKAYDEAFAAHWKAETGETVTIQQSHGGSGAQARAVI
DGLDADVVTLALEGDINAIVSKSKKINPDWRKKFENNSAPYTSTIIFLVRKGNPKGIHDWSDLVKDGTQVITPNPKTSGG
ARWNYLAAWAYANAHDGGDEAKTKEFVGKLYANAPVLDTGARGSTVTFAQKGIGDVLIGWENDAYLALDEFGADNFEIVY
PPTSILAEPPVAVVDANVDAKGTRKVAEAYLGWLYSKEGQTIIAKNHYRPAKPELVAAVDLPKTPDIKLISIDDPLFGGW
KKAQPYHFGDGGIFDQIYKPAQ
>Mature_341_residues
TKPHFRKLLGALVATSVQFGTLGFAFADTTILNVSYDPTRELYKAYDEAFAAHWKAETGETVTIQQSHGGSGAQARAVID
GLDADVVTLALEGDINAIVSKSKKINPDWRKKFENNSAPYTSTIIFLVRKGNPKGIHDWSDLVKDGTQVITPNPKTSGGA
RWNYLAAWAYANAHDGGDEAKTKEFVGKLYANAPVLDTGARGSTVTFAQKGIGDVLIGWENDAYLALDEFGADNFEIVYP
PTSILAEPPVAVVDANVDAKGTRKVAEAYLGWLYSKEGQTIIAKNHYRPAKPELVAAVDLPKTPDIKLISIDDPLFGGWK
KAQPYHFGDGGIFDQIYKPAQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=311, Percent_Identity=62.0578778135048, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1788765, Length=309, Percent_Identity=48.2200647249191, Blast_Score=291, Evalue=6e-80,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37134; Mature: 37003

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPHFRKLLGALVATSVQFGTLGFAFADTTILNVSYDPTRELYKAYDEAFAAHWKAETG
CCCHHHHHHHHHHHHHCCCCCCEEEEEECEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
ETVTIQQSHGGSGAQARAVIDGLDADVVTLALEGDINAIVSKSKKINPDWRKKFENNSAP
CEEEEEECCCCCCCCHHHEECCCCCCEEEEEEECCHHHHHHCCCCCCHHHHHHHCCCCCC
YTSTIIFLVRKGNPKGIHDWSDLVKDGTQVITPNPKTSGGARWNYLAAWAYANAHDGGDE
EEEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEEECCCCCCCCH
AKTKEFVGKLYANAPVLDTGARGSTVTFAQKGIGDVLIGWENDAYLALDEFGADNFEIVY
HHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCEEEEECCCCEEEEECCCCCCCEEEE
PPTSILAEPPVAVVDANVDAKGTRKVAEAYLGWLYSKEGQTIIAKNHYRPAKPELVAAVD
CCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEC
LPKTPDIKLISIDDPLFGGWKKAQPYHFGDGGIFDQIYKPAQ
CCCCCCEEEEEECCCCCCCCCCCCCEECCCCCHHHHHCCCCC
>Mature Secondary Structure 
TKPHFRKLLGALVATSVQFGTLGFAFADTTILNVSYDPTRELYKAYDEAFAAHWKAETG
CCHHHHHHHHHHHHHCCCCCCEEEEEECEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
ETVTIQQSHGGSGAQARAVIDGLDADVVTLALEGDINAIVSKSKKINPDWRKKFENNSAP
CEEEEEECCCCCCCCHHHEECCCCCCEEEEEEECCHHHHHHCCCCCCHHHHHHHCCCCCC
YTSTIIFLVRKGNPKGIHDWSDLVKDGTQVITPNPKTSGGARWNYLAAWAYANAHDGGDE
EEEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEEECCCCCCCCH
AKTKEFVGKLYANAPVLDTGARGSTVTFAQKGIGDVLIGWENDAYLALDEFGADNFEIVY
HHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCEEEEECCCCEEEEECCCCCCCEEEE
PPTSILAEPPVAVVDANVDAKGTRKVAEAYLGWLYSKEGQTIIAKNHYRPAKPELVAAVD
CCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEC
LPKTPDIKLISIDDPLFGGWKKAQPYHFGDGGIFDQIYKPAQ
CCCCCCEEEEEECCCCCCCCCCCCCEECCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]