Click here to switch to the map view.

The map label for this gene is vanX [H]

Identifier: 13471635

GI number: 13471635

Start: 1396583

End: 1397314

Strand: Direct

Name: vanX [H]

Synonym: mlr1665

Alternate gene names: 13471635

Gene position: 1396583-1397314 (Clockwise)

Preceding gene: 13471634

Following gene: 13471636

Centisome position: 19.85

GC content: 67.08

Gene sequence:

>732_bases
TTGAATAGAGCGCGCCAGGCGATTCTGCGCGTTGGCTTTTACGCGCTGACGGTTGCCGTTGCCGTTCTGCCGGCACATGC
CGACCCTCTCCCCGCCGGCTTCGTCCGCCTCGCCGATATCGCCCCGTCGATCCGCCAGGACATCCGCTACGCCGGTGCAG
GCAATTTCCTGCACCGCAAGGTGACCGGCTACGACGCCCCCGTCTGCATTCTCACCTTGCAGGCGGCGCAAGCCCTTGCC
GGCGTCCAGCAGGCAATCGCCGCTCGGGGCCTGACGCTGGTCGTCTTCGACTGCTATCGCCCCGCCCGTGCCGTCGCCGA
CATGGGCGAGTGGACGCGACAGGGCGGGCCGCCCGATCCGCAATGGTATCCCGGGGTCCGGCGTGGCGACCTCATCGCCA
AGGGCTATGTCGGCGAACTGTCGACCCATTCGCGCGGTTCGACCGTCGATGTCGCGATTGCGCGGGCCGACGGTCCATCC
GTCGCCAAACCCGCCTGCGGCGCCATCGATGCCGATACGCTCGACTTCGGCAGCGGTTTCGACTGTTTCGACCCGATGAG
CGAGACGGCGCACCGCCCGCTCGGCAATGAAGCGACAGAAAACCGCAGGCTGCTCGTCGAGGCCATGCGCGCCGGCGGTT
TCAAGAACTACGCGCGCGAATGGTGGCACTATACGCTCCGGCATGAGCCCTTTCCCACCCAGCGCTTCGATTTCCCGGTA
GGCGCCGAGTGA

Upstream 100 bases:

>100_bases
GATCGCCGCCGCACCGCGACGTGGTTGCCCGACGGCGCTGGTTATTCGACCCTGACCGTGATCGACGCGGCCGGGCGAGC
GGCCAGCGTGAAAGTGTTCG

Downstream 100 bases:

>100_bases
TTTGCCGGACCAGGCGACCTTGCCTCGGCACCGCGCATCCCTAGATAGGACCTCGAATCCGCCCCGAGCGCCGGCGAGGA
AAATATTTCCCAACGCGACG

Product: D-alanyl-D-alanine dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanX [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNRARQAILRVGFYALTVAVAVLPAHADPLPAGFVRLADIAPSIRQDIRYAGAGNFLHRKVTGYDAPVCILTLQAAQALA
GVQQAIAARGLTLVVFDCYRPARAVADMGEWTRQGGPPDPQWYPGVRRGDLIAKGYVGELSTHSRGSTVDVAIARADGPS
VAKPACGAIDADTLDFGSGFDCFDPMSETAHRPLGNEATENRRLLVEAMRAGGFKNYAREWWHYTLRHEPFPTQRFDFPV
GAE

Sequences:

>Translated_243_residues
MNRARQAILRVGFYALTVAVAVLPAHADPLPAGFVRLADIAPSIRQDIRYAGAGNFLHRKVTGYDAPVCILTLQAAQALA
GVQQAIAARGLTLVVFDCYRPARAVADMGEWTRQGGPPDPQWYPGVRRGDLIAKGYVGELSTHSRGSTVDVAIARADGPS
VAKPACGAIDADTLDFGSGFDCFDPMSETAHRPLGNEATENRRLLVEAMRAGGFKNYAREWWHYTLRHEPFPTQRFDFPV
GAE
>Mature_243_residues
MNRARQAILRVGFYALTVAVAVLPAHADPLPAGFVRLADIAPSIRQDIRYAGAGNFLHRKVTGYDAPVCILTLQAAQALA
GVQQAIAARGLTLVVFDCYRPARAVADMGEWTRQGGPPDPQWYPGVRRGDLIAKGYVGELSTHSRGSTVDVAIARADGPS
VAKPACGAIDADTLDFGSGFDCFDPMSETAHRPLGNEATENRRLLVEAMRAGGFKNYAREWWHYTLRHEPFPTQRFDFPV
GAE

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=193, Percent_Identity=31.6062176165803, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 26379; Mature: 26379

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRARQAILRVGFYALTVAVAVLPAHADPLPAGFVRLADIAPSIRQDIRYAGAGNFLHRK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCHHHHC
VTGYDAPVCILTLQAAQALAGVQQAIAARGLTLVVFDCYRPARAVADMGEWTRQGGPPDP
CCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC
QWYPGVRRGDLIAKGYVGELSTHSRGSTVDVAIARADGPSVAKPACGAIDADTLDFGSGF
CCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DCFDPMSETAHRPLGNEATENRRLLVEAMRAGGFKNYAREWWHYTLRHEPFPTQRFDFPV
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GAE
CCC
>Mature Secondary Structure
MNRARQAILRVGFYALTVAVAVLPAHADPLPAGFVRLADIAPSIRQDIRYAGAGNFLHRK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHCCCCCHHHHC
VTGYDAPVCILTLQAAQALAGVQQAIAARGLTLVVFDCYRPARAVADMGEWTRQGGPPDP
CCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC
QWYPGVRRGDLIAKGYVGELSTHSRGSTVDVAIARADGPSVAKPACGAIDADTLDFGSGF
CCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DCFDPMSETAHRPLGNEATENRRLLVEAMRAGGFKNYAREWWHYTLRHEPFPTQRFDFPV
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380148; 7854121; 7873524 [H]