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The map label for this gene is proS

Identifier: 13471387

GI number: 13471387

Start: 1125520

End: 1126848

Strand: Reverse

Name: proS

Synonym: mll1344

Alternate gene names: 13471387

Gene position: 1126848-1125520 (Counterclockwise)

Preceding gene: 13471388

Following gene: 13471386

Centisome position: 16.02

GC content: 62.3

Gene sequence:

>1329_bases
ATGCGTTTGTCGCGCTATTTCCTGCCTATCCTCAAAGAAAATCCGCGCGAGGCCGAGATCGTCTCACATCGGCTGATGCT
GCGTGCCGGCATGATCCGCCAGCAGGGGCAGGGCAGTTTTTCCTGGCTGCCACTTGGCAAGCGGGTGCTGGACAAGGTCT
GCCAGATCATCCGCGAGGAGCAGAACCGCGCCGGCGCGCTGGAGATCCTGATGCCGACCATCCAGTCGGCCGATCTGTGG
CGCGAAAGCGGCCGCTACAATGACTATGGCAAGGAGATGCTGCGCATCAAGGACCGCCAGGATCGCGACATGCTCTACGG
CCCGACCAACGAGGAAATGGTCACCGAGATCTTCCGCGCCTATGTGAAATCCTACAAGGATTTGCCGCTCAACCTCTACC
ACATCCAGTGGAAGTTCCGTGACGAGGTGCGGCCGCGCTTCGGCGTCATGCGTTCGCGCGAGTTCCTGATGAAGGACGCC
TATTCCTTCGACCTCGACTTCGAGGGCGCGAGGTCTGCATACAACCGGATGTTCGTCTCCTACCTCAGGACCTTTACCCG
CATGGGCCTGCAGGCGATTCCCATGCGCGCCGACACCGGGCCGATCGGCGGCGACCTCAGCCACGAATTCATCATCCTGG
CCGATACCGGCGAAAGCCAGGTTTTCTGCCATCGTGACTATCTTTCGCTTGCCGTGCCGGGCGCCAATACCGATTTCGCC
AATGACGGCGAGATCGCGGATATCGTCAAGACATGGACGACGCCCTACGCCGCCACCGACGAGATGCATGACGAAGCCGC
CTGGGAAAAGGTGCCCGAGAGCGACAAGGTTTCGGCACGCGGCATCGAGGTTGGCCACATCTTCCATTTCGGCGAGAAGT
ATTCCAAGCCGATGGGCGCCAAGGTGACCGGACCGGACGGCAAGGACCATTTCGCCTCCGGCGGCTCCTACGGCATCGGC
CCGTCGCGCCTGGTCGCGGCGATCATCGAAGCCAGCCATGATGAGAACGGCATCATCTGGCCCGACGCCGTGGCGCCGTT
CGACATCGGCCTGATCAACATGAAAGTGGGCGACGCCGATTGCGACCGTGTCAGCGAAGAGCTGAATGCCGCCTTCACCG
CGGCCGGCAAGGATGTTCTCTATGACGACACCGACCAGCGGCCGGGCGGCAAGTTCGCCACAGCCGACCTGATCGGGCTG
CCCTGGCAAGTGATCGTCGGACCGCGCGGCGTGGCCGCCGGCGAGGTCGAGATCAAGAATCGCAGGACCGGCGAACGCGA
AACGCTGCCCATCGCCGACGCGAAAAAACGCTTTGGTGTCGCCGCATGA

Upstream 100 bases:

>100_bases
CCCGGGTTTCCATTCCGTCATGAAATGGCTATGAAGCCGGGGCAAGCAAGCCTGCGTCGCGCCGATTCTGGCGTGGCCGT
TCTCATTCACGGACCAGTCC

Downstream 100 bases:

>100_bases
GCGAGGCGGCGGCGGCAAGATCGGCGGGCGCCGGCCCGTTCTCCTTCTTCGAGCGCACGGTTGCCTGGCGCTATTTGCGC
TCGCGGCGCAAGGAGACGGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRLSRYFLPILKENPREAEIVSHRLMLRAGMIRQQGQGSFSWLPLGKRVLDKVCQIIREEQNRAGALEILMPTIQSADLW
RESGRYNDYGKEMLRIKDRQDRDMLYGPTNEEMVTEIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDFEGARSAYNRMFVSYLRTFTRMGLQAIPMRADTGPIGGDLSHEFIILADTGESQVFCHRDYLSLAVPGANTDFA
NDGEIADIVKTWTTPYAATDEMHDEAAWEKVPESDKVSARGIEVGHIFHFGEKYSKPMGAKVTGPDGKDHFASGGSYGIG
PSRLVAAIIEASHDENGIIWPDAVAPFDIGLINMKVGDADCDRVSEELNAAFTAAGKDVLYDDTDQRPGGKFATADLIGL
PWQVIVGPRGVAAGEVEIKNRRTGERETLPIADAKKRFGVAA

Sequences:

>Translated_442_residues
MRLSRYFLPILKENPREAEIVSHRLMLRAGMIRQQGQGSFSWLPLGKRVLDKVCQIIREEQNRAGALEILMPTIQSADLW
RESGRYNDYGKEMLRIKDRQDRDMLYGPTNEEMVTEIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDFEGARSAYNRMFVSYLRTFTRMGLQAIPMRADTGPIGGDLSHEFIILADTGESQVFCHRDYLSLAVPGANTDFA
NDGEIADIVKTWTTPYAATDEMHDEAAWEKVPESDKVSARGIEVGHIFHFGEKYSKPMGAKVTGPDGKDHFASGGSYGIG
PSRLVAAIIEASHDENGIIWPDAVAPFDIGLINMKVGDADCDRVSEELNAAFTAAGKDVLYDDTDQRPGGKFATADLIGL
PWQVIVGPRGVAAGEVEIKNRRTGERETLPIADAKKRFGVAA
>Mature_442_residues
MRLSRYFLPILKENPREAEIVSHRLMLRAGMIRQQGQGSFSWLPLGKRVLDKVCQIIREEQNRAGALEILMPTIQSADLW
RESGRYNDYGKEMLRIKDRQDRDMLYGPTNEEMVTEIFRAYVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDFEGARSAYNRMFVSYLRTFTRMGLQAIPMRADTGPIGGDLSHEFIILADTGESQVFCHRDYLSLAVPGANTDFA
NDGEIADIVKTWTTPYAATDEMHDEAAWEKVPESDKVSARGIEVGHIFHFGEKYSKPMGAKVTGPDGKDHFASGGSYGIG
PSRLVAAIIEASHDENGIIWPDAVAPFDIGLINMKVGDADCDRVSEELNAAFTAAGKDVLYDDTDQRPGGKFATADLIGL
PWQVIVGPRGVAAGEVEIKNRRTGERETLPIADAKKRFGVAA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=425, Percent_Identity=33.1764705882353, Blast_Score=239, Evalue=3e-63,
Organism=Escherichia coli, GI1786392, Length=262, Percent_Identity=52.6717557251908, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=399, Percent_Identity=32.0802005012531, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI193203271, Length=95, Percent_Identity=36.8421052631579, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=213, Percent_Identity=36.150234741784, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24656200, Length=422, Percent_Identity=30.5687203791469, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_RHILO (Q98KS5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102953.1
- ProteinModelPortal:   Q98KS5
- SMR:   Q98KS5
- GeneID:   1225616
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll1344
- NMPDR:   fig|266835.1.peg.1058
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49631; Mature: 49631

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPILKENPREAEIVSHRLMLRAGMIRQQGQGSFSWLPLGKRVLDKVCQIIREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
QNRAGALEILMPTIQSADLWRESGRYNDYGKEMLRIKDRQDRDMLYGPTNEEMVTEIFRA
HCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHH
YVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDLDFEGARSAYNRMFVS
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YLRTFTRMGLQAIPMRADTGPIGGDLSHEFIILADTGESQVFCHRDYLSLAVPGANTDFA
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCEEEEECCCCCCCC
NDGEIADIVKTWTTPYAATDEMHDEAAWEKVPESDKVSARGIEVGHIFHFGEKYSKPMGA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEHEEECHHHHCCCCCC
KVTGPDGKDHFASGGSYGIGPSRLVAAIIEASHDENGIIWPDAVAPFDIGLINMKVGDAD
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEEEECCCH
CDRVSEELNAAFTAAGKDVLYDDTDQRPGGKFATADLIGLPWQVIVGPRGVAAGEVEIKN
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEHHHHCCCEEEEECCCCCCCCEEEECC
RRTGERETLPIADAKKRFGVAA
CCCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MRLSRYFLPILKENPREAEIVSHRLMLRAGMIRQQGQGSFSWLPLGKRVLDKVCQIIREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
QNRAGALEILMPTIQSADLWRESGRYNDYGKEMLRIKDRQDRDMLYGPTNEEMVTEIFRA
HCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHH
YVKSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDLDFEGARSAYNRMFVS
HHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YLRTFTRMGLQAIPMRADTGPIGGDLSHEFIILADTGESQVFCHRDYLSLAVPGANTDFA
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCEEEEECCCCCCCC
NDGEIADIVKTWTTPYAATDEMHDEAAWEKVPESDKVSARGIEVGHIFHFGEKYSKPMGA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEHEEECHHHHCCCCCC
KVTGPDGKDHFASGGSYGIGPSRLVAAIIEASHDENGIIWPDAVAPFDIGLINMKVGDAD
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEEEECCCH
CDRVSEELNAAFTAAGKDVLYDDTDQRPGGKFATADLIGLPWQVIVGPRGVAAGEVEIKN
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEHHHHCCCEEEEECCCCCCCCEEEECC
RRTGERETLPIADAKKRFGVAA
CCCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968