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The map label for this gene is rffG [C]

Identifier: 13471322

GI number: 13471322

Start: 1061305

End: 1062312

Strand: Reverse

Name: rffG [C]

Synonym: mll1262

Alternate gene names: 13471322

Gene position: 1062312-1061305 (Counterclockwise)

Preceding gene: 13471323

Following gene: 13471321

Centisome position: 15.1

GC content: 61.61

Gene sequence:

>1008_bases
ATGAGACATGTGATTTTCGGCGGTGACGGCTTCGTCGGCCGTCACCTTGCCCCGAAGCTTGTGGCGGATGGCGAAGAGGT
AATCGTCGCCGACATCGCCAGGAGCGACCTTGCGCACTACCGCAATGTCCGGTTCGTCCAATGCGACGTCACGGATTCGG
CTTCGGTCGCTGCTGTCGGGCTCAAGGCTGGCGACATGGTCTACAATCTGTCGGCCAAGATGCTGTCGCCGATCCAGGTG
CGCGCCAAGCGGCACGACTTCTTTTTCCCAGTTAATTTCCATGGCACCGAGCACATCATGCAGGCCATGGACAGGGCTGG
TGCGCGAAAACTCGTCCACTACACAACGGATATGATCTACGGCCACACGGTCACCCAGCCGATGACCGAGGAACACCCGG
TCGCGCCGCTCGGCGAATATGGCTGGTCGAAGCAGAAGACCGAGGAACTCGCCGCCGAATGGCGCAAGCGCGGCATGTCG
ATCTCGCTGTTCCGGCCGCGCCTGATCATCGGCCCCGGCCGGCTCGGCATTCTCGAAAAACTGTTCAAGCTGATTGACTG
GAACCTTCCGGTGCCGATGATCGGCTTGGGTAAAAACCCCTATCAGTTCATTTCCGTTTTCGATTGCGCGGAAGCTGCCC
GCGCCGCCTGGAAGGCCGGCGTGCCCAACGAGGCCTATAATCTCGGTTCGCTCAACCCGCCGCCGGTGAAGAAATTGCTC
GGCGACCTGATCCGCCATGCCGGCTCGAAGTCGATCCTGCTGCCGACGCCGGGCTGGGCGGTCAAGCGCACGCTCGACCT
GCTCGACCTCATGAACATGCCGATCATGGATCCGGAGCAATATCTGATCGCCGACGAGGAATGCGTACTCGACGTGTCCA
AGGCCGAGCGCCAACTCGGCTGGGTGCCGCAATATCGCGACGAGGACATGCTGATCGCCGCCTACAGCGAGTACCGCGCC
AAGAACGCCGGGCCTGCTGTCGCCACCAAACATGTGCCCGCCGAGTAG

Upstream 100 bases:

>100_bases
TCTGCGTCGGCACCGTGCTGATCGCGCAAAGTGGGCGAGGGCATGAGGACCAGACCGCTTCCATCACGCCCGACAAGACC
CAGATAAGCGAGACCGTTCG

Downstream 100 bases:

>100_bases
TGGTCCCGCCAAATTTATGGCGCACGACGCTGGTCGTAGCGCGGACAGGAGATTGAAATGACCGTCATGGCCAAGCCCGA
ACAGACGAATGCGCGCACCC

Product: NDP-glucose 4,6-dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRHVIFGGDGFVGRHLAPKLVADGEEVIVADIARSDLAHYRNVRFVQCDVTDSASVAAVGLKAGDMVYNLSAKMLSPIQV
RAKRHDFFFPVNFHGTEHIMQAMDRAGARKLVHYTTDMIYGHTVTQPMTEEHPVAPLGEYGWSKQKTEELAAEWRKRGMS
ISLFRPRLIIGPGRLGILEKLFKLIDWNLPVPMIGLGKNPYQFISVFDCAEAARAAWKAGVPNEAYNLGSLNPPPVKKLL
GDLIRHAGSKSILLPTPGWAVKRTLDLLDLMNMPIMDPEQYLIADEECVLDVSKAERQLGWVPQYRDEDMLIAAYSEYRA
KNAGPAVATKHVPAE

Sequences:

>Translated_335_residues
MRHVIFGGDGFVGRHLAPKLVADGEEVIVADIARSDLAHYRNVRFVQCDVTDSASVAAVGLKAGDMVYNLSAKMLSPIQV
RAKRHDFFFPVNFHGTEHIMQAMDRAGARKLVHYTTDMIYGHTVTQPMTEEHPVAPLGEYGWSKQKTEELAAEWRKRGMS
ISLFRPRLIIGPGRLGILEKLFKLIDWNLPVPMIGLGKNPYQFISVFDCAEAARAAWKAGVPNEAYNLGSLNPPPVKKLL
GDLIRHAGSKSILLPTPGWAVKRTLDLLDLMNMPIMDPEQYLIADEECVLDVSKAERQLGWVPQYRDEDMLIAAYSEYRA
KNAGPAVATKHVPAE
>Mature_335_residues
MRHVIFGGDGFVGRHLAPKLVADGEEVIVADIARSDLAHYRNVRFVQCDVTDSASVAAVGLKAGDMVYNLSAKMLSPIQV
RAKRHDFFFPVNFHGTEHIMQAMDRAGARKLVHYTTDMIYGHTVTQPMTEEHPVAPLGEYGWSKQKTEELAAEWRKRGMS
ISLFRPRLIIGPGRLGILEKLFKLIDWNLPVPMIGLGKNPYQFISVFDCAEAARAAWKAGVPNEAYNLGSLNPPPVKKLL
GDLIRHAGSKSILLPTPGWAVKRTLDLLDLMNMPIMDPEQYLIADEECVLDVSKAERQLGWVPQYRDEDMLIAAYSEYRA
KNAGPAVATKHVPAE

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI7657641, Length=258, Percent_Identity=24.8062015503876, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37299; Mature: 37299

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHVIFGGDGFVGRHLAPKLVADGEEVIVADIARSDLAHYRNVRFVQCDVTDSASVAAVG
CCEEEECCCCCCCCCCCCHHHCCCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
LKAGDMVYNLSAKMLSPIQVRAKRHDFFFPVNFHGTEHIMQAMDRAGARKLVHYTTDMIY
CCCCCEEEECHHHHCCHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
GHTVTQPMTEEHPVAPLGEYGWSKQKTEELAAEWRKRGMSISLFRPRLIIGPGRLGILEK
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEECCCCHHHHHH
LFKLIDWNLPVPMIGLGKNPYQFISVFDCAEAARAAWKAGVPNEAYNLGSLNPPPVKKLL
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
GDLIRHAGSKSILLPTPGWAVKRTLDLLDLMNMPIMDPEQYLIADEECVLDVSKAERQLG
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHEEECHHHEEEHHHHHHHHC
WVPQYRDEDMLIAAYSEYRAKNAGPAVATKHVPAE
CCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRHVIFGGDGFVGRHLAPKLVADGEEVIVADIARSDLAHYRNVRFVQCDVTDSASVAAVG
CCEEEECCCCCCCCCCCCHHHCCCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
LKAGDMVYNLSAKMLSPIQVRAKRHDFFFPVNFHGTEHIMQAMDRAGARKLVHYTTDMIY
CCCCCEEEECHHHHCCHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
GHTVTQPMTEEHPVAPLGEYGWSKQKTEELAAEWRKRGMSISLFRPRLIIGPGRLGILEK
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEECCCCHHHHHH
LFKLIDWNLPVPMIGLGKNPYQFISVFDCAEAARAAWKAGVPNEAYNLGSLNPPPVKKLL
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
GDLIRHAGSKSILLPTPGWAVKRTLDLLDLMNMPIMDPEQYLIADEECVLDVSKAERQLG
HHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHEEECHHHEEEHHHHHHHHC
WVPQYRDEDMLIAAYSEYRAKNAGPAVATKHVPAE
CCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]