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The map label for this gene is holB [H]

Identifier: 13470653

GI number: 13470653

Start: 305542

End: 306591

Strand: Reverse

Name: holB [H]

Synonym: mll0423

Alternate gene names: 13470653

Gene position: 306591-305542 (Counterclockwise)

Preceding gene: 13470654

Following gene: 13470650

Centisome position: 4.36

GC content: 64.95

Gene sequence:

>1050_bases
ATGATCTTCGAACGCATCGCGCCGGAACAGCATGATACGCTGGACGGCGTGCCCGAACCGTCCGAAACGCCCCGTCTGGT
CGGGCACGGGCAGGCGGCCGAGATGCTCGCCGCCGCCTATCGATCTGGAAAGCTGCCGCATGCGCTGATCTTCGCCGGAC
CGGTCGGCATCGGCAAGGCGACGCTGGCCTTCCACCTGGCGCATCACCTCCTGAAGCACCCGGCCTTCGAGCAGGCGCCG
GAACGCCTGGCCAATCCCGATCCGGCCTCGCCGCTGTTTCGCCAGATCGCGACCGGGGCGCATCCGGGCGTGCTGCACCT
GACTAGACCGATGAACGACAAGACCAAGAGCTTCAAGACGGTCGTCACCGTCGATGAGATCCGCAGGGTCAGCCGCTTCC
TGTCGATGACCTCGCATGACGGCAGCTACCGGGTCGTCATCGTCGATCCAGCCGACGACATGAATGCCAATGCCGCCAAT
GCCTTGCTGAAGAATCTTGAGGAGCCGCCGGCGCGAACGCTGTTCATTCTCATCGTCCATGCGCCGGGCAGCCTGCTGCC
GACGATCCGCTCACGCTGCCAGGTGATACGCCTGACACCGCTTGACGCCGATGATTTGCTGGCGGTTCTGGAGACCGCCG
AACCGCCACCGCCGGACGATCCGGCCGCGCGGGCGGCACTGGTTGAGCGGGCAGGGGGCAGCGCCCGCAACGCGATCCTG
TTGACGCAATATGGCGGGCTGGAGATCGCCGGGACGCTCGATGCGCTGGTGGCCAAGGGAAAGAGCGACATCGGCGGAGC
CTATCGGCTCGCGGAAGCCGTTGCCGGCCGCGACCAGACGATCCAGTTCGATATCTTCAACCGCCGGGCGCTCGACTTGT
TGTCGGATGCCGCGAGCCAGGCAGCGCTGTCGGGCGATCTCGCGCGGGCCAAAATCCTGTCGGATACCTGGCATGAGGCG
CTGGATGCTATCTCCGAGACCGACACCTACAATCTCGACAAGAAGCAGCACGCCCTGACAATGATCGACCGGCTGAAGTC
GGTGATGTAA

Upstream 100 bases:

>100_bases
CCTCCGCCGATCCCAATACGGTGGAGGACGTCGTCACCGCGGCCGTCTTCGCGGCGCTGGAGGCGAGGGCGCCTGCGCGC
AACAGGCAGGCCGCACCGGT

Downstream 100 bases:

>100_bases
ATATCCTTTCGGATGGCAATCGCCATTCATGCGGCCTGCTTTTGGGACCATCTTCTAGGACTGGGTTGCCCGCTGCACCG
CAAGCGCGGAATCATACTTC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIFERIAPEQHDTLDGVPEPSETPRLVGHGQAAEMLAAAYRSGKLPHALIFAGPVGIGKATLAFHLAHHLLKHPAFEQAP
ERLANPDPASPLFRQIATGAHPGVLHLTRPMNDKTKSFKTVVTVDEIRRVSRFLSMTSHDGSYRVVIVDPADDMNANAAN
ALLKNLEEPPARTLFILIVHAPGSLLPTIRSRCQVIRLTPLDADDLLAVLETAEPPPPDDPAARAALVERAGGSARNAIL
LTQYGGLEIAGTLDALVAKGKSDIGGAYRLAEAVAGRDQTIQFDIFNRRALDLLSDAASQAALSGDLARAKILSDTWHEA
LDAISETDTYNLDKKQHALTMIDRLKSVM

Sequences:

>Translated_349_residues
MIFERIAPEQHDTLDGVPEPSETPRLVGHGQAAEMLAAAYRSGKLPHALIFAGPVGIGKATLAFHLAHHLLKHPAFEQAP
ERLANPDPASPLFRQIATGAHPGVLHLTRPMNDKTKSFKTVVTVDEIRRVSRFLSMTSHDGSYRVVIVDPADDMNANAAN
ALLKNLEEPPARTLFILIVHAPGSLLPTIRSRCQVIRLTPLDADDLLAVLETAEPPPPDDPAARAALVERAGGSARNAIL
LTQYGGLEIAGTLDALVAKGKSDIGGAYRLAEAVAGRDQTIQFDIFNRRALDLLSDAASQAALSGDLARAKILSDTWHEA
LDAISETDTYNLDKKQHALTMIDRLKSVM
>Mature_349_residues
MIFERIAPEQHDTLDGVPEPSETPRLVGHGQAAEMLAAAYRSGKLPHALIFAGPVGIGKATLAFHLAHHLLKHPAFEQAP
ERLANPDPASPLFRQIATGAHPGVLHLTRPMNDKTKSFKTVVTVDEIRRVSRFLSMTSHDGSYRVVIVDPADDMNANAAN
ALLKNLEEPPARTLFILIVHAPGSLLPTIRSRCQVIRLTPLDADDLLAVLETAEPPPPDDPAARAALVERAGGSARNAIL
LTQYGGLEIAGTLDALVAKGKSDIGGAYRLAEAVAGRDQTIQFDIFNRRALDLLSDAASQAALSGDLARAKILSDTWHEA
LDAISETDTYNLDKKQHALTMIDRLKSVM

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=219, Percent_Identity=28.310502283105, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787341, Length=167, Percent_Identity=34.1317365269461, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71986063, Length=194, Percent_Identity=27.8350515463918, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37594; Mature: 37594

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFERIAPEQHDTLDGVPEPSETPRLVGHGQAAEMLAAAYRSGKLPHALIFAGPVGIGKA
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCHH
TLAFHLAHHLLKHPAFEQAPERLANPDPASPLFRQIATGAHPGVLHLTRPMNDKTKSFKT
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHH
VVTVDEIRRVSRFLSMTSHDGSYRVVIVDPADDMNANAANALLKNLEEPPARTLFILIVH
EEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
APGSLLPTIRSRCQVIRLTPLDADDLLAVLETAEPPPPDDPAARAALVERAGGSARNAIL
CCCCHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEE
LTQYGGLEIAGTLDALVAKGKSDIGGAYRLAEAVAGRDQTIQFDIFNRRALDLLSDAASQ
EEECCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHH
AALSGDLARAKILSDTWHEALDAISETDTYNLDKKQHALTMIDRLKSVM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFERIAPEQHDTLDGVPEPSETPRLVGHGQAAEMLAAAYRSGKLPHALIFAGPVGIGKA
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCHH
TLAFHLAHHLLKHPAFEQAPERLANPDPASPLFRQIATGAHPGVLHLTRPMNDKTKSFKT
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHH
VVTVDEIRRVSRFLSMTSHDGSYRVVIVDPADDMNANAANALLKNLEEPPARTLFILIVH
EEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEE
APGSLLPTIRSRCQVIRLTPLDADDLLAVLETAEPPPPDDPAARAALVERAGGSARNAIL
CCCCHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEE
LTQYGGLEIAGTLDALVAKGKSDIGGAYRLAEAVAGRDQTIQFDIFNRRALDLLSDAASQ
EEECCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHH
AALSGDLARAKILSDTWHEALDAISETDTYNLDKKQHALTMIDRLKSVM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]