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The map label for this gene is metG

Identifier: 13470650

GI number: 13470650

Start: 301681

End: 303231

Strand: Reverse

Name: metG

Synonym: mll0419

Alternate gene names: 13470650

Gene position: 303231-301681 (Counterclockwise)

Preceding gene: 13470653

Following gene: 13470649

Centisome position: 4.31

GC content: 61.64

Gene sequence:

>1551_bases
ATGTCACGCGAAAAATACTATCTGACGACACCGATTTTCTATCCGAACGGCAAGCCGCATATCGGCCATGCCTATACGGT
CATCGCAACCGATGCGCTGGCCCGCTTCCAGCGCCTCGACGGCAAGGACGTGTTCTTCCTCACCGGCACGGACGAACACG
GCCTCAAGATGCAGCAGACGGCTGAGAAGGAAGGCATCACGCCACAAGCGCTTGCCGACCGCAATTCAGCGATCTTCCGT
TCGATGACCGAGGCTGTGGGCGGCTCGAATGATGAGTACATCCGCACGACGGAACCGCGCCACTACGCGTCCTGCCAGGC
GATCTGGAAGGCGATGGCCGCCAATGGCGACATCTATCTGGACCGCTATAGCGGCTGGTATTCGGTCCGCCAGGAAGCCT
ATTTCGACGAGAGCGAAACCACGCTCGGCGAGGACGGCGTGCGCCGCGAGCCGCTTGGCTCGCCGGTCGAATGGAACGAG
GAAGAAAGCTATTTCTTCCGGCTCTCCGCCTATCAGGACAAACTGCTGGCGCTGTACGAAAGCCAGCCCGACTTTGTCGG
ACCTGCCGAGCGTCGCAACGAGGTGATGAGTTTCGTCAAATCCGGGCTGAAAGACCTGTCGATCTCGCGCACGACCTTCA
AATGGGGCGTGCCGGTGCCCGGCGACGACAAGCATGTGATGTATGTCTGGGTCGATGCCTTGACCAACTACATCACCGCC
GCCGGCTATCCGGACACAAAGTCCGATCAATGGCGCTACTGGCCGGCCACGCACATCATCGGCAAAGATATCGTGCGCTT
CCACGCGGTCTACTGGCCGGCTTTCCTGATGTCGGCCGGCATCGAACTGCCGAAGCGTGTTTTCGCCCACGGCTTCCTGT
TCAACCGCGGCGAGAAGATGTCGAAGTCGGTCGGCAATGTCGTCGATCCGTTCGCCCTGATCGAGCATTATGGCCTCGAC
CAGGTGCGCTATTTCTTCCTGCGCGAAGTGCCGTTCGGCCAGGATGGCAGCTACAGCCATGATGCGATCGTCAACCGCAC
CAACGCCGATCTCGCCAATGGCCTCGGCAATCTGGCGCAGCGCTCGCTGTCGATGATCGCCAAGAACTGCGGCGGCGTGG
TGCCGAAGCGTGGCGAGATGACGGATGCCGACAGTGCCATCCTGGATCAAGCGGTGGCCGCTCTGACCACCGCGCGGAAG
GCGATGGCCGGGCAAGGCATCCATCTGGCACTGGCGGCGATCTTCGATGTGGTGGCGGAAGCCGATCGATACTTTGCTGG
ACAGGCACCATGGGCGCTGAAGAAAACCGATCCGGAGCGGATGGAAACGGTGCTGTGGACGACCGCCGAACTGGTGCGGC
GCGTGGCGGTGCTGTGCCAGCCCTTCATTCCGGGATCGGCGGCGAAGCTGCTCGACCTGCTGGCGGTGCCCGCGGATAAA
CGTGATTTCGTGCATGTCCACGCCGACTATGCGTTGGTTCCTGGCACTGCGTTGCCCGCGCCGGAGGGCGTGTTTCCGCG
TTATGTCGAACAGCCGGGTGCGAACGCCTGA

Upstream 100 bases:

>100_bases
CTACGATGCGAATGTGACGGCCCCACCGGGATGGCAATCGCCATTCCGATGCGATATCCACCGCCACGGCTTCCATTCAG
ACATTCATGATGGTTCGTTC

Downstream 100 bases:

>100_bases
TGCTCGTCGACAGCCATTGCCATCTGGACTTTCCGGATTTCGCCGAAGAGCGGGCGGCCATTGTCGCCCGCGCCAAAGCG
GCTGGTATCGGCCGCATGGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSREKYYLTTPIFYPNGKPHIGHAYTVIATDALARFQRLDGKDVFFLTGTDEHGLKMQQTAEKEGITPQALADRNSAIFR
SMTEAVGGSNDEYIRTTEPRHYASCQAIWKAMAANGDIYLDRYSGWYSVRQEAYFDESETTLGEDGVRREPLGSPVEWNE
EESYFFRLSAYQDKLLALYESQPDFVGPAERRNEVMSFVKSGLKDLSISRTTFKWGVPVPGDDKHVMYVWVDALTNYITA
AGYPDTKSDQWRYWPATHIIGKDIVRFHAVYWPAFLMSAGIELPKRVFAHGFLFNRGEKMSKSVGNVVDPFALIEHYGLD
QVRYFFLREVPFGQDGSYSHDAIVNRTNADLANGLGNLAQRSLSMIAKNCGGVVPKRGEMTDADSAILDQAVAALTTARK
AMAGQGIHLALAAIFDVVAEADRYFAGQAPWALKKTDPERMETVLWTTAELVRRVAVLCQPFIPGSAAKLLDLLAVPADK
RDFVHVHADYALVPGTALPAPEGVFPRYVEQPGANA

Sequences:

>Translated_516_residues
MSREKYYLTTPIFYPNGKPHIGHAYTVIATDALARFQRLDGKDVFFLTGTDEHGLKMQQTAEKEGITPQALADRNSAIFR
SMTEAVGGSNDEYIRTTEPRHYASCQAIWKAMAANGDIYLDRYSGWYSVRQEAYFDESETTLGEDGVRREPLGSPVEWNE
EESYFFRLSAYQDKLLALYESQPDFVGPAERRNEVMSFVKSGLKDLSISRTTFKWGVPVPGDDKHVMYVWVDALTNYITA
AGYPDTKSDQWRYWPATHIIGKDIVRFHAVYWPAFLMSAGIELPKRVFAHGFLFNRGEKMSKSVGNVVDPFALIEHYGLD
QVRYFFLREVPFGQDGSYSHDAIVNRTNADLANGLGNLAQRSLSMIAKNCGGVVPKRGEMTDADSAILDQAVAALTTARK
AMAGQGIHLALAAIFDVVAEADRYFAGQAPWALKKTDPERMETVLWTTAELVRRVAVLCQPFIPGSAAKLLDLLAVPADK
RDFVHVHADYALVPGTALPAPEGVFPRYVEQPGANA
>Mature_515_residues
SREKYYLTTPIFYPNGKPHIGHAYTVIATDALARFQRLDGKDVFFLTGTDEHGLKMQQTAEKEGITPQALADRNSAIFRS
MTEAVGGSNDEYIRTTEPRHYASCQAIWKAMAANGDIYLDRYSGWYSVRQEAYFDESETTLGEDGVRREPLGSPVEWNEE
ESYFFRLSAYQDKLLALYESQPDFVGPAERRNEVMSFVKSGLKDLSISRTTFKWGVPVPGDDKHVMYVWVDALTNYITAA
GYPDTKSDQWRYWPATHIIGKDIVRFHAVYWPAFLMSAGIELPKRVFAHGFLFNRGEKMSKSVGNVVDPFALIEHYGLDQ
VRYFFLREVPFGQDGSYSHDAIVNRTNADLANGLGNLAQRSLSMIAKNCGGVVPKRGEMTDADSAILDQAVAALTTARKA
MAGQGIHLALAAIFDVVAEADRYFAGQAPWALKKTDPERMETVLWTTAELVRRVAVLCQPFIPGSAAKLLDLLAVPADKR
DFVHVHADYALVPGTALPAPEGVFPRYVEQPGANA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=38.6274509803922, Blast_Score=334, Evalue=9e-92,
Organism=Homo sapiens, GI14043022, Length=517, Percent_Identity=26.3056092843327, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1788432, Length=501, Percent_Identity=25.748502994012, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1786861, Length=178, Percent_Identity=28.0898876404494, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=36.3106796116505, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71992781, Length=405, Percent_Identity=35.3086419753086, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17540794, Length=527, Percent_Identity=27.134724857685, Blast_Score=162, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6321610, Length=529, Percent_Identity=32.1361058601134, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=23.9208633093525, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21358483, Length=518, Percent_Identity=36.8725868725869, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24655311, Length=514, Percent_Identity=25.875486381323, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21355409, Length=178, Percent_Identity=29.7752808988764, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_RHILO (Q98MV8)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102219.1
- ProteinModelPortal:   Q98MV8
- GeneID:   1224882
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0419
- NMPDR:   fig|266835.1.peg.324
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK11893
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 57526; Mature: 57394

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREKYYLTTPIFYPNGKPHIGHAYTVIATDALARFQRLDGKDVFFLTGTDEHGLKMQQT
CCCCCEEEECCEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
AEKEGITPQALADRNSAIFRSMTEAVGGSNDEYIRTTEPRHYASCQAIWKAMAANGDIYL
HHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEE
DRYSGWYSVRQEAYFDESETTLGEDGVRREPLGSPVEWNEEESYFFRLSAYQDKLLALYE
ECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHC
SQPDFVGPAERRNEVMSFVKSGLKDLSISRTTFKWGVPVPGDDKHVMYVWVDALTNYITA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHHHEE
AGYPDTKSDQWRYWPATHIIGKDIVRFHAVYWPAFLMSAGIELPKRVFAHGFLFNRGEKM
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
SKSVGNVVDPFALIEHYGLDQVRYFFLREVPFGQDGSYSHDAIVNRTNADLANGLGNLAQ
HHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHH
RSLSMIAKNCGGVVPKRGEMTDADSAILDQAVAALTTARKAMAGQGIHLALAAIFDVVAE
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ADRYFAGQAPWALKKTDPERMETVLWTTAELVRRVAVLCQPFIPGSAAKLLDLLAVPADK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
RDFVHVHADYALVPGTALPAPEGVFPRYVEQPGANA
CCEEEEEECEEEECCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
SREKYYLTTPIFYPNGKPHIGHAYTVIATDALARFQRLDGKDVFFLTGTDEHGLKMQQT
CCCCEEEECCEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
AEKEGITPQALADRNSAIFRSMTEAVGGSNDEYIRTTEPRHYASCQAIWKAMAANGDIYL
HHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEE
DRYSGWYSVRQEAYFDESETTLGEDGVRREPLGSPVEWNEEESYFFRLSAYQDKLLALYE
ECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHC
SQPDFVGPAERRNEVMSFVKSGLKDLSISRTTFKWGVPVPGDDKHVMYVWVDALTNYITA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHHHEE
AGYPDTKSDQWRYWPATHIIGKDIVRFHAVYWPAFLMSAGIELPKRVFAHGFLFNRGEKM
CCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
SKSVGNVVDPFALIEHYGLDQVRYFFLREVPFGQDGSYSHDAIVNRTNADLANGLGNLAQ
HHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHH
RSLSMIAKNCGGVVPKRGEMTDADSAILDQAVAALTTARKAMAGQGIHLALAAIFDVVAE
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ADRYFAGQAPWALKKTDPERMETVLWTTAELVRRVAVLCQPFIPGSAAKLLDLLAVPADK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
RDFVHVHADYALVPGTALPAPEGVFPRYVEQPGANA
CCEEEEEECEEEECCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968