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The map label for this gene is tmk

Identifier: 13470654

GI number: 13470654

Start: 306588

End: 307262

Strand: Reverse

Name: tmk

Synonym: mll0424

Alternate gene names: 13470654

Gene position: 307262-306588 (Counterclockwise)

Preceding gene: 13470655

Following gene: 13470653

Centisome position: 4.37

GC content: 67.7

Gene sequence:

>675_bases
TTGGCGCGCGGATTTTTCATCACCTTTGAAGGCGGCGAGGGCGCAGGCAAGTCGACGCAGATCGAGCGGCTGGCGCGGAA
GATGCACGCCAAGAAGTATGATGTCCTGCTGACGCGCGAACCGGGCGGCTCGCCGGGCGCCGAAGCGGTCAGGCATGTGC
TTCTCTCAGGCGCCGCCGAGCCATTCGGACCGAAGATGGAGGCGCTGCTCTTCGCCGCCGCGCGTTCCGACCATGTCGAA
CAGGTTATCCGGCCGGCGGTCGAGCGCGGCTCGATCGTGCTTTGCGATCGTTTCCTGGATTCCTCGCGCGTCTACCAGGG
CGTCACCGGCGGGATCGACCCGGCGTTCATGGCCACGCTGGAACAGATCGCCATCAACGGCATGATGCCCGATATGACGC
TGATCTTCGACATCGATCCGGCCGAGGGCCTCAGGCGCGCGACGCTGCGGCGCGGCAGCGACGCCGGCGCCGACCGCTTC
GAAAAGGAGACTCTGGCCATCCATCAGGCCCGCCGCGCGGCCTTTCTGGCGATCGCCGCGGCCGAACCGGAACGCTGCAT
CGTCGTCGATGCCTCCGCCGATCCCAATACGGTGGAGGACGTCGTCACCGCGGCCGTCTTCGCGGCGCTGGAGGCGAGGG
CGCCTGCGCGCAACAGGCAGGCCGCACCGGTATGA

Upstream 100 bases:

>100_bases
CTGGCGATCGGCTGGCTGCGATAGCACCGACGGCATGGACGTTTTCCCGAACGCGGACTATCTAGAGCCCAACAATCCAC
ATGGACAAAGGCCCTTTCGT

Downstream 100 bases:

>100_bases
TCTTCGAACGCATCGCGCCGGAACAGCATGATACGCTGGACGGCGTGCCCGAACCGTCCGAAACGCCCCGTCTGGTCGGG
CACGGGCAGGCGGCCGAGAT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MARGFFITFEGGEGAGKSTQIERLARKMHAKKYDVLLTREPGGSPGAEAVRHVLLSGAAEPFGPKMEALLFAAARSDHVE
QVIRPAVERGSIVLCDRFLDSSRVYQGVTGGIDPAFMATLEQIAINGMMPDMTLIFDIDPAEGLRRATLRRGSDAGADRF
EKETLAIHQARRAAFLAIAAAEPERCIVVDASADPNTVEDVVTAAVFAALEARAPARNRQAAPV

Sequences:

>Translated_224_residues
MARGFFITFEGGEGAGKSTQIERLARKMHAKKYDVLLTREPGGSPGAEAVRHVLLSGAAEPFGPKMEALLFAAARSDHVE
QVIRPAVERGSIVLCDRFLDSSRVYQGVTGGIDPAFMATLEQIAINGMMPDMTLIFDIDPAEGLRRATLRRGSDAGADRF
EKETLAIHQARRAAFLAIAAAEPERCIVVDASADPNTVEDVVTAAVFAALEARAPARNRQAAPV
>Mature_223_residues
ARGFFITFEGGEGAGKSTQIERLARKMHAKKYDVLLTREPGGSPGAEAVRHVLLSGAAEPFGPKMEALLFAAARSDHVEQ
VIRPAVERGSIVLCDRFLDSSRVYQGVTGGIDPAFMATLEQIAINGMMPDMTLIFDIDPAEGLRRATLRRGSDAGADRFE
KETLAIHQARRAAFLAIAAAEPERCIVVDASADPNTVEDVVTAAVFAALEARAPARNRQAAPV

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=214, Percent_Identity=35.981308411215, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_RHILO (Q98MV4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102223.1
- ProteinModelPortal:   Q98MV4
- SMR:   Q98MV4
- GeneID:   1224886
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0424
- NMPDR:   fig|266835.1.peg.328
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK13973
- BRENDA:   2.7.4.9
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 24002; Mature: 23870

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGFFITFEGGEGAGKSTQIERLARKMHAKKYDVLLTREPGGSPGAEAVRHVLLSGAAE
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
PFGPKMEALLFAAARSDHVEQVIRPAVERGSIVLCDRFLDSSRVYQGVTGGIDPAFMATL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCHHHHHHCCCCCCCHHHHHHH
EQIAINGMMPDMTLIFDIDPAEGLRRATLRRGSDAGADRFEKETLAIHQARRAAFLAIAA
HHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
AEPERCIVVDASADPNTVEDVVTAAVFAALEARAPARNRQAAPV
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ARGFFITFEGGEGAGKSTQIERLARKMHAKKYDVLLTREPGGSPGAEAVRHVLLSGAAE
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
PFGPKMEALLFAAARSDHVEQVIRPAVERGSIVLCDRFLDSSRVYQGVTGGIDPAFMATL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCHHHHHHCCCCCCCHHHHHHH
EQIAINGMMPDMTLIFDIDPAEGLRRATLRRGSDAGADRFEKETLAIHQARRAAFLAIAA
HHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEE
AEPERCIVVDASADPNTVEDVVTAAVFAALEARAPARNRQAAPV
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968