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The map label for this gene is purH [H]

Identifier: 134302692

GI number: 134302692

Start: 1768885

End: 1770432

Strand: Direct

Name: purH [H]

Synonym: FTW_1885

Alternate gene names: 134302692

Gene position: 1768885-1770432 (Clockwise)

Preceding gene: 134302689

Following gene: 134302695

Centisome position: 93.17

GC content: 36.63

Gene sequence:

>1548_bases
ATGTCAAAAATAAAAAGGGTACTAATATCTGTATCTGATAAAACAGGTATCATTGAATTTGCCAAAGAATTAAGTAAGTA
TAACATCGAAATACTTTCAACAGGTGGAACTTCAAAAACCCTTAAAGATGCTGGAATAAACGTAATAGATGTTTCAGAGC
ATACACAATTTCCAGAAATTATGAATGGTCGTGTAAAAACACTTCATCCACTAATTCATGGTGGTATCTTAGCCGATAGA
GATAATCCTGAGCATCTCGATGCGATGCTAAAAAATCATATTGGACAAATAGATATGGTAGTCGTTAACTTATACCCTTT
TGTCAATACAGTTAAATCCGGTGCTGATTTTGATACCTGTATCGAAAACATAGATATCGGCGGACCTTCTATGGTGAGAT
CATGTGCAAAAAATCATAAACACACAACTATAGTGACCAACCCTAGTCAATATGATCTAGTTATCAAAGAGATGGCTGAG
AATGATGGCAAAACTACTCTAGCAACACGTCGTGAATTTGCTAAAGAAGCGTTTGCACATACTGCCGAATATGATAGTCA
TATTGCAAACTGGTTTAGTAAAGAGCTAGATGAAGAGTATCCTGAGAAACTTTTTTCTGTAGGAACTCTAAAACAGGTTT
TAAGATATGGCGAAAATCCTCATCAAAAAGCAGCTGTATACTCTACAGATGCTGACTGTGTAAGCATTACAAATGCAAGA
CAATTACAAGGCAAAGAATTATCGTATAATAACCTAAATGATGCCGATGGTGCTTTTGAGATGGTTTGTGAGTATTCAGA
ACCCTCTTGTGCAATTATCAAACATGCTAATCCTTGTGGTATCGCTTCTGGTAAAAATGCTTTTGAAGCTTGGACAAAAG
CTCTAGCTTGTGATCCAATTTCAGCTTTTGGTGGAATTGTTGCGTTTAACTGTGAAGTTGACAAAGAGTTTGCTGAATCT
CTAGGTAAAATGTTTTTAGAAGTAATAATTGCACCAAGCTATACTCAAGAAGCTCTAGATATTTTAGCAGCTAAGAAAAA
CTTACGCGTATTAGAAACTGGTAAAGTTTTTGATACAAAATCATCACGTATGATAAGTAAAAATATTCATGGTGGAATAC
TATCTCAATCTTATGATAACGGTGGCGTTGACATTAATGACTGTAAAGTTGTTACAGATAGAGCGCCAACTGACAAAGAG
TGGGCTGATTTAATGTTTGCCTGGAAAGCTGTTAAATACGTCAAATCAAATGCAATTGTCTATGCTAGAGATACCCAAAC
AGTAGGAATTGGTGCTGGACAGATGAGTCGAGTTGATTCTGCACGTATTGGTGCTGAAAAAGCCCAAGCTCATCAAGGTG
CTAAAGGTTCTGCTGTCGCATCTGATGCTTTCTTCCCTTTTGCTGACGGTTTAGAAGAATGTATCAAAGCTGGAGCAACA
GCGGTAATTCAACCAGGCGGTTCAAAAAATGATCAAGAAGTTATTGATGCTGCTAACAAAGCTGGTATCACTATGGTCTT
CACAAATATGAGACATTTTAGACACTAA

Upstream 100 bases:

>100_bases
ATATCGTTTAACAAAAATGTTTTAACTAAGAATTTATGATTAAAATTGTTATAATGATAGTTAAAATCTGACTTTAAATA
AATAATAACAAGGAACAAGC

Downstream 100 bases:

>100_bases
ATTAATAAATCTTTCTCCAAGTTGGATAATGTTATTTATTGTATTTTAATTGCTAGAACATTCTTTTAAGATAACGCCGA
AGTTAAAAGCGGCGATATTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEIMNGRVKTLHPLIHGGILADR
DNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTCIENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAE
NDGKTTLATRREFAKEAFAHTAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADCVSITNAR
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPISAFGGIVAFNCEVDKEFAES
LGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTKSSRMISKNIHGGILSQSYDNGGVDINDCKVVTDRAPTDKE
WADLMFAWKAVKYVKSNAIVYARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH

Sequences:

>Translated_515_residues
MSKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEIMNGRVKTLHPLIHGGILADR
DNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTCIENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAE
NDGKTTLATRREFAKEAFAHTAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADCVSITNAR
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPISAFGGIVAFNCEVDKEFAES
LGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTKSSRMISKNIHGGILSQSYDNGGVDINDCKVVTDRAPTDKE
WADLMFAWKAVKYVKSNAIVYARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH
>Mature_514_residues
SKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEIMNGRVKTLHPLIHGGILADRD
NPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTCIENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAEN
DGKTTLATRREFAKEAFAHTAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADCVSITNARQ
LQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPISAFGGIVAFNCEVDKEFAESL
GKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTKSSRMISKNIHGGILSQSYDNGGVDINDCKVVTDRAPTDKEW
ADLMFAWKAVKYVKSNAIVYARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGATA
VIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=38.2845188284519, Blast_Score=286, Evalue=4e-77,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=52.5519848771267, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71985564, Length=602, Percent_Identity=30.5647840531561, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=343, Percent_Identity=27.1137026239067, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=35.5179704016913, Blast_Score=259, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=35.3065539112051, Blast_Score=256, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24649832, Length=477, Percent_Identity=36.8972746331237, Blast_Score=262, Evalue=4e-70,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56220; Mature: 56089

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEI
CCHHHEEHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCEEECCCCCCCHHH
MNGRVKTLHPLIHGGILADRDNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTC
HCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCHHHH
IENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAENDGKTTLATRREFAKEAFAH
HHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADCVSITNAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCHH
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPI
HCCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCH
SAFGGIVAFNCEVDKEFAESLGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTK
HHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEECCC
SSRMISKNIHGGILSQSYDNGGVDINDCKVVTDRAPTDKEWADLMFAWKAVKYVKSNAIV
CCHHHHHCCCCCHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
YARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
EEECCCEEECCCCHHHHHHHHHCCHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCE
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH
EEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
SKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEI
CHHHEEHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCEEECCCCCCCHHH
MNGRVKTLHPLIHGGILADRDNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTC
HCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCHHHH
IENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAENDGKTTLATRREFAKEAFAH
HHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADCVSITNAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCHH
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPI
HCCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCH
SAFGGIVAFNCEVDKEFAESLGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTK
HHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEECCC
SSRMISKNIHGGILSQSYDNGGVDINDCKVVTDRAPTDKEWADLMFAWKAVKYVKSNAIV
CCHHHHHCCCCCHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
YARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
EEECCCEEECCCCHHHHHHHHHCCHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCE
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH
EEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA