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The map label for this gene is holA [C]

Identifier: 134302695

GI number: 134302695

Start: 1775162

End: 1776139

Strand: Direct

Name: holA [C]

Synonym: FTW_1894

Alternate gene names: 134302695

Gene position: 1775162-1776139 (Clockwise)

Preceding gene: 134302692

Following gene: 134302696

Centisome position: 93.5

GC content: 30.78

Gene sequence:

>978_bases
ATGGAATTAAGTTATTTTGAGCTACTCCAAAAAAATGATCTCACCGCCTATAAACTTTTTATCATAACAGGTGATGAACC
TCTACAAAAACACAATACTATTGAAAAAATAACTAATCAGTTTAAAACTAAAAATTTTGAAATAAGCTATCATGATTTAA
GTGAACAAAATATAGATGTTTTGTATAACGAAGTTGATAGTCTTAGCCTATTCAGTATTGATAAATTTATTCAATTCAAT
TTTGATAAGCCCCCACAAAAAAAACTCCAACAAACATTAGTTGACAAGCTTATAAATGATGATGATAACGTTTACTTGCT
TGTTTTTAGTGGTATGAAAAAGCAAAATACCTCAGCAAAGTGGTTTCAAAGCTTAGAGCATAAAGCTATTCATATTCGCA
TTTTTCAACCAAATTTAGATAATGCCATAAATATTATAGATTATGAAACACAACAACTCGGCTTAAGTCTGACAAAAGAA
GCTACACAGCTCTTAGCGCTAAAAACCGAGGGAAATTTGATAGCAACAAAACAAATCTTAAAATTACTCTCACGCCAAGA
TAGCCGAGTCTTTGATGAAAATACAATTCGTCCTTTTCTACATGAGCACGCCAATTTTGACGTATTTGATCTTTCTGAAG
CAATTCTCTCTCAGCATAAAAGCAAAGCACTAAAGATACTTAATAGCATCCTGAACGAAAATGATAAGTCTCCTCTTGTA
CTCTGGGCCCTAAAAAGAGAATTAAGAATACTCTCCCAACTAAAAAACACTCAAATAACTTATCATCAAAAAATATTTAA
AGATAACAATATTTGGTCAGCTAAACAGAAATTTTATATTAGTTTAGCGAATAAGCTTAGTCCTGAAAAAATTTCAGCAG
GACTGGAAAAATGCCTTGATACTGACCTATGTATCAAAGGTGCAAGAAAAGGCAATATTCAGCTTAAACTTAATGAAATT
GTTTTTGATATTTTCTAA

Upstream 100 bases:

>100_bases
AACTAACTTTTTCATAATTAAACCTCTTTTGATTTCTTATATGCTAAACAGTATAGTAAACTAACTGTGATATATAACAA
TAGCTAAAAGTTTTAATATT

Downstream 100 bases:

>100_bases
ATACACTATCTAAGATTTTTGCTAAATTTTAATGACAACTGTAATAGCAGCATTTACTTTTTTTAGACAATTGATATCAT
TAAACAGAATTAACCAATGT

Product: DNA polymerase III, delta subunit

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Subunit Delta; DNA Polymerase III Delta Subunit Probably ATP Hydrolase; DNA-Directed DNA Polymerase; DNA Polymerase III

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MELSYFELLQKNDLTAYKLFIITGDEPLQKHNTIEKITNQFKTKNFEISYHDLSEQNIDVLYNEVDSLSLFSIDKFIQFN
FDKPPQKKLQQTLVDKLINDDDNVYLLVFSGMKKQNTSAKWFQSLEHKAIHIRIFQPNLDNAINIIDYETQQLGLSLTKE
ATQLLALKTEGNLIATKQILKLLSRQDSRVFDENTIRPFLHEHANFDVFDLSEAILSQHKSKALKILNSILNENDKSPLV
LWALKRELRILSQLKNTQITYHQKIFKDNNIWSAKQKFYISLANKLSPEKISAGLEKCLDTDLCIKGARKGNIQLKLNEI
VFDIF

Sequences:

>Translated_325_residues
MELSYFELLQKNDLTAYKLFIITGDEPLQKHNTIEKITNQFKTKNFEISYHDLSEQNIDVLYNEVDSLSLFSIDKFIQFN
FDKPPQKKLQQTLVDKLINDDDNVYLLVFSGMKKQNTSAKWFQSLEHKAIHIRIFQPNLDNAINIIDYETQQLGLSLTKE
ATQLLALKTEGNLIATKQILKLLSRQDSRVFDENTIRPFLHEHANFDVFDLSEAILSQHKSKALKILNSILNENDKSPLV
LWALKRELRILSQLKNTQITYHQKIFKDNNIWSAKQKFYISLANKLSPEKISAGLEKCLDTDLCIKGARKGNIQLKLNEI
VFDIF
>Mature_325_residues
MELSYFELLQKNDLTAYKLFIITGDEPLQKHNTIEKITNQFKTKNFEISYHDLSEQNIDVLYNEVDSLSLFSIDKFIQFN
FDKPPQKKLQQTLVDKLINDDDNVYLLVFSGMKKQNTSAKWFQSLEHKAIHIRIFQPNLDNAINIIDYETQQLGLSLTKE
ATQLLALKTEGNLIATKQILKLLSRQDSRVFDENTIRPFLHEHANFDVFDLSEAILSQHKSKALKILNSILNENDKSPLV
LWALKRELRILSQLKNTQITYHQKIFKDNNIWSAKQKFYISLANKLSPEKISAGLEKCLDTDLCIKGARKGNIQLKLNEI
VFDIF

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Delta Subunit Seems To Interact With The Gamma Subunit To Transfer The B

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=275, Percent_Identity=24.7272727272727, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 37765; Mature: 37765

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSYFELLQKNDLTAYKLFIITGDEPLQKHNTIEKITNQFKTKNFEISYHDLSEQNIDV
CCCHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHH
LYNEVDSLSLFSIDKFIQFNFDKPPQKKLQQTLVDKLINDDDNVYLLVFSGMKKQNTSAK
HHHHHCCEEHEEHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHH
WFQSLEHKAIHIRIFQPNLDNAINIIDYETQQLGLSLTKEATQLLALKTEGNLIATKQIL
HHHHHCCCEEEEEEECCCCCCCEEEEEECHHHHCCHHHHHHHHEEEEECCCCEEHHHHHH
KLLSRQDSRVFDENTIRPFLHEHANFDVFDLSEAILSQHKSKALKILNSILNENDKSPLV
HHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LWALKRELRILSQLKNTQITYHQKIFKDNNIWSAKQKFYISLANKLSPEKISAGLEKCLD
EHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEECHHHHHHEEHHHCCCHHHHHHHHHHHCC
TDLCIKGARKGNIQLKLNEIVFDIF
CHHHHCCCCCCCEEEEEEHEEEECC
>Mature Secondary Structure
MELSYFELLQKNDLTAYKLFIITGDEPLQKHNTIEKITNQFKTKNFEISYHDLSEQNIDV
CCCHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHH
LYNEVDSLSLFSIDKFIQFNFDKPPQKKLQQTLVDKLINDDDNVYLLVFSGMKKQNTSAK
HHHHHCCEEHEEHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHH
WFQSLEHKAIHIRIFQPNLDNAINIIDYETQQLGLSLTKEATQLLALKTEGNLIATKQIL
HHHHHCCCEEEEEEECCCCCCCEEEEEECHHHHCCHHHHHHHHEEEEECCCCEEHHHHHH
KLLSRQDSRVFDENTIRPFLHEHANFDVFDLSEAILSQHKSKALKILNSILNENDKSPLV
HHHCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LWALKRELRILSQLKNTQITYHQKIFKDNNIWSAKQKFYISLANKLSPEKISAGLEKCLD
EHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEECHHHHHHEEHHHCCCHHHHHHHHHHHCC
TDLCIKGARKGNIQLKLNEIVFDIF
CHHHHCCCCCCCEEEEEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA