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The map label for this gene is gapA [H]

Identifier: 134301590

GI number: 134301590

Start: 510162

End: 511208

Strand: Direct

Name: gapA [H]

Synonym: FTW_0523

Alternate gene names: 134301590

Gene position: 510162-511208 (Clockwise)

Preceding gene: 134301589

Following gene: 134301591

Centisome position: 26.87

GC content: 37.73

Gene sequence:

>1047_bases
ATGTTTAATAAACTTTCGCAAGATAATATCTTAAGGAGATTAAAAATGAGAGTTGCAATTAATGGTTTCGGTAGAATTGG
TCGTTTAGCATTTCGTCAAATGTTTGGTAAAGATAACATTGAGATTGTCGCGATTAATGATTTAACAAATCCTGAGATGT
TAGCACACTTATTGAAATATGATTCTGCTCAAGGTAGATTCTCAAAAGCAGCTGTAACAGTAGCAAAAGAAAGCTCTATA
GTTGTCGATGGTAAAGAGATTAAAATATATGCTGAAAAAGATGCAGCTAATCTACCTTGGGGCGAGTTAAATGTCGATGT
TGTACTAGAGTGCACTGGTTTTTATGTATCAAAAGCAAAGTCACAAGCTCATATTGATGCAGGTGCTAAAAAAGTAGTTA
TTTCAGCGCCAGCTGGTAATGACCTACCAACAGTTGTCTTTGGTGTTAATCATGACATCCTCAGCGCAGATGATAAAATC
ATATCAGCGGCTTCTTGTACAACTAATTGTCTAGCTCCTATGGCTAAAGCTCTTCATGATCTAGCAACCATTGAAAGTGG
TTTTATGACCACTATTCATGCATATACAGGTGACCAAAATACTTTAGATGCTCCTCATGCGAAAAATGACTTCCGTCGTG
CAAGAGCTGCTGCTGTAAATATTGTACCTAACTCAACTGGTGCCGCTAAAGCTATTGGTTTAGTGATTCCTGAACTTGCT
GGTAAGTTAGATGGGGCTGCTCAGCGTGTCCCTGTTGCTACTGGTTCTTTAACTGAGCTTGTTGCTGTCGTATCTAAGAA
GGTAACAGCTGAAGATGTTAATGCTGCGATGAAAGCTGCTGCAAATAAATCTTTTGGTTACACAGAAGAAGAGCTAGTGT
CTAGTGATATCATCGGGATTTCTGAAGGATCGTTATTCGATGCAACTCAAACTAAAGTTACTTCACTAGGTGATAAATCT
CTAGTTAAAGTAGTATCTTGGTATGACAATGAAATGTCTTATACAAATCAAATGGTTAGAGTAGTAGAGTACTTCGGAGC
TCTATAA

Upstream 100 bases:

>100_bases
GTAAAAGGAATCTATAGCTTTGGTGAGTCAGCACCGGCTGAAGATTTATTTAAACGTTTTGGATTTACTGTAGAAAATAT
TAGCAATATTGTTGCTAAAT

Downstream 100 bases:

>100_bases
ACATTATAGGTGATATAAATGAGTTTTCTAACATTGAAAGATGTTGATTTAAAAGATAAAAAAGTCTTGGTTCGTGTTGA
CTTCAACGTACCTGTTAAAG

Product: glyceraldehyde-3-phosphate dehydrogenase, type I

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MFNKLSQDNILRRLKMRVAINGFGRIGRLAFRQMFGKDNIEIVAINDLTNPEMLAHLLKYDSAQGRFSKAAVTVAKESSI
VVDGKEIKIYAEKDAANLPWGELNVDVVLECTGFYVSKAKSQAHIDAGAKKVVISAPAGNDLPTVVFGVNHDILSADDKI
ISAASCTTNCLAPMAKALHDLATIESGFMTTIHAYTGDQNTLDAPHAKNDFRRARAAAVNIVPNSTGAAKAIGLVIPELA
GKLDGAAQRVPVATGSLTELVAVVSKKVTAEDVNAAMKAAANKSFGYTEEELVSSDIIGISEGSLFDATQTKVTSLGDKS
LVKVVSWYDNEMSYTNQMVRVVEYFGAL

Sequences:

>Translated_348_residues
MFNKLSQDNILRRLKMRVAINGFGRIGRLAFRQMFGKDNIEIVAINDLTNPEMLAHLLKYDSAQGRFSKAAVTVAKESSI
VVDGKEIKIYAEKDAANLPWGELNVDVVLECTGFYVSKAKSQAHIDAGAKKVVISAPAGNDLPTVVFGVNHDILSADDKI
ISAASCTTNCLAPMAKALHDLATIESGFMTTIHAYTGDQNTLDAPHAKNDFRRARAAAVNIVPNSTGAAKAIGLVIPELA
GKLDGAAQRVPVATGSLTELVAVVSKKVTAEDVNAAMKAAANKSFGYTEEELVSSDIIGISEGSLFDATQTKVTSLGDKS
LVKVVSWYDNEMSYTNQMVRVVEYFGAL
>Mature_348_residues
MFNKLSQDNILRRLKMRVAINGFGRIGRLAFRQMFGKDNIEIVAINDLTNPEMLAHLLKYDSAQGRFSKAAVTVAKESSI
VVDGKEIKIYAEKDAANLPWGELNVDVVLECTGFYVSKAKSQAHIDAGAKKVVISAPAGNDLPTVVFGVNHDILSADDKI
ISAASCTTNCLAPMAKALHDLATIESGFMTTIHAYTGDQNTLDAPHAKNDFRRARAAAVNIVPNSTGAAKAIGLVIPELA
GKLDGAAQRVPVATGSLTELVAVVSKKVTAEDVNAAMKAAANKSFGYTEEELVSSDIIGISEGSLFDATQTKVTSLGDKS
LVKVVSWYDNEMSYTNQMVRVVEYFGAL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=336, Percent_Identity=43.1547619047619, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI7657116, Length=337, Percent_Identity=43.026706231454, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1788079, Length=336, Percent_Identity=46.7261904761905, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789295, Length=335, Percent_Identity=43.2835820895522, Blast_Score=252, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI32566163, Length=342, Percent_Identity=45.3216374269006, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17568413, Length=342, Percent_Identity=45.3216374269006, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17534677, Length=340, Percent_Identity=45.5882352941176, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17534679, Length=340, Percent_Identity=45.5882352941176, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321631, Length=335, Percent_Identity=48.3582089552239, Blast_Score=303, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=47.3053892215569, Blast_Score=300, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=47.0059880239521, Blast_Score=296, Evalue=3e-81,
Organism=Drosophila melanogaster, GI19922412, Length=332, Percent_Identity=44.8795180722892, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=44.0119760479042, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=44.0119760479042, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=44.0119760479042, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=44.0119760479042, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 37229; Mature: 37229

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKLSQDNILRRLKMRVAINGFGRIGRLAFRQMFGKDNIEIVAINDLTNPEMLAHLLKY
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHC
DSAQGRFSKAAVTVAKESSIVVDGKEIKIYAEKDAANLPWGELNVDVVLECTGFYVSKAK
CCCCCCHHHHEEEEEECCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHCC
SQAHIDAGAKKVVISAPAGNDLPTVVFGVNHDILSADDKIISAASCTTNCLAPMAKALHD
CHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHH
LATIESGFMTTIHAYTGDQNTLDAPHAKNDFRRARAAAVNIVPNSTGAAKAIGLVIPELA
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHH
GKLDGAAQRVPVATGSLTELVAVVSKKVTAEDVNAAMKAAANKSFGYTEEELVSSDIIGI
CCCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEC
SEGSLFDATQTKVTSLGDKSLVKVVSWYDNEMSYTNQMVRVVEYFGAL
CCCCEECCHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNKLSQDNILRRLKMRVAINGFGRIGRLAFRQMFGKDNIEIVAINDLTNPEMLAHLLKY
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHC
DSAQGRFSKAAVTVAKESSIVVDGKEIKIYAEKDAANLPWGELNVDVVLECTGFYVSKAK
CCCCCCHHHHEEEEEECCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHCC
SQAHIDAGAKKVVISAPAGNDLPTVVFGVNHDILSADDKIISAASCTTNCLAPMAKALHD
CHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHH
LATIESGFMTTIHAYTGDQNTLDAPHAKNDFRRARAAAVNIVPNSTGAAKAIGLVIPELA
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHH
GKLDGAAQRVPVATGSLTELVAVVSKKVTAEDVNAAMKAAANKSFGYTEEELVSSDIIGI
CCCCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEC
SEGSLFDATQTKVTSLGDKSLVKVVSWYDNEMSYTNQMVRVVEYFGAL
CCCCEECCHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10463150; 11466286 [H]