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The map label for this gene is rpoA1 [H]

Identifier: 134301520

GI number: 134301520

Start: 438763

End: 439719

Strand: Reverse

Name: rpoA1 [H]

Synonym: FTW_0446

Alternate gene names: 134301520

Gene position: 439719-438763 (Counterclockwise)

Preceding gene: 134301521

Following gene: 134301518

Centisome position: 23.16

GC content: 32.71

Gene sequence:

>957_bases
ATGGCATTAGAAAATTTACTACATCCTACTAATATTAAAATTGATGAATATGCTAAAAATGCTACTAAGTTTTCTTTTGA
AGCCCTAGAGAGAGGGGTAGGTTATACACTTGGGTTTGCTCTTAAGCAAACTATGCTTTACTCTATTGCTGGTGCATGTG
TTACTAGTATAAAAATTAATGATGGTAAAGTTACTTCTTTAGAAGATGTGATTCCATGTGATGAGACAGTTGCTGATATT
ATCCTGAACGTTAAATCTCTGCCTGTAACTTTAGCAGAAGGTGTTGAAACGGGGACAATAACTTTTGAACTATCAGGCTC
GGAAGAAGAAATTTTTTCAGAAGAAGCTAAGCTATCTGAAGGTTTGGCAATAACTGAAGAAGTATTTATCTGTAGTTACA
ATGGTGGTAAAAAGCTAAAAATAGAAGCTAAAGTAGAAAAAGGCGTAGGCTTTAGACCTGCACAAGATAATTTTAAGGAT
GGTGAGTTCCTTTTAGATGCGACTTTCTCACCTGTTGTTTTTTGTGATTTTGAGATTAAAGATGCTCGTGTTGGTAGACG
TACGGATCTTGATAAGCTTGAGCTTAATATTAAAACAAATGGTAATGTGAATTGCGAGGAAGCTTTAAGACTAGCAGCTA
CTAAAATCCAAAATCAATTAAGAAATATCGTAGATATTGAAGAGATTAATAAGGGTATTTTTGTAGAAGATCCTACTAAA
GATATAAATCCTATTTTATTAAAGCATGTTGAAGAATTAAATCTAACAGCTAGATCTTCTAATTGCTTAAAAGCTGTAAA
TATTAGACTAATTGGTGAGTTAGTACAGAAGACAGAAAACGAACTTCTTAAAGCACCAAATTTTGGTAAAAAATCATTAA
CAGAGATCAAAGACAAGTTGTCTGAGCTTGGTTTATCTTTAGGAACTCTTATTGAAAATTGGCCTCAAGATTTATAA

Upstream 100 bases:

>100_bases
CAAGAAGTTGTAAAATTAAGTTATTAAATTTTTGTATTTGTATGTTATAATCTTTGGAATTAAATTATATCTATAAAATA
TAACTAGGTTTTAAACTGCT

Downstream 100 bases:

>100_bases
TCACTTCTGCCGGAGTGGTGAAATTGGTAGACGCGCCGGATTCAAAATCCGGTTCTGGCAACAGAGTGCCGGTTCGAGTC
CGGTCTCCGGCACCATTGAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha 2; RNA polymerase subunit alpha 2; Transcriptase subunit alpha 2 [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MALENLLHPTNIKIDEYAKNATKFSFEALERGVGYTLGFALKQTMLYSIAGACVTSIKINDGKVTSLEDVIPCDETVADI
ILNVKSLPVTLAEGVETGTITFELSGSEEEIFSEEAKLSEGLAITEEVFICSYNGGKKLKIEAKVEKGVGFRPAQDNFKD
GEFLLDATFSPVVFCDFEIKDARVGRRTDLDKLELNIKTNGNVNCEEALRLAATKIQNQLRNIVDIEEINKGIFVEDPTK
DINPILLKHVEELNLTARSSNCLKAVNIRLIGELVQKTENELLKAPNFGKKSLTEIKDKLSELGLSLGTLIENWPQDL

Sequences:

>Translated_318_residues
MALENLLHPTNIKIDEYAKNATKFSFEALERGVGYTLGFALKQTMLYSIAGACVTSIKINDGKVTSLEDVIPCDETVADI
ILNVKSLPVTLAEGVETGTITFELSGSEEEIFSEEAKLSEGLAITEEVFICSYNGGKKLKIEAKVEKGVGFRPAQDNFKD
GEFLLDATFSPVVFCDFEIKDARVGRRTDLDKLELNIKTNGNVNCEEALRLAATKIQNQLRNIVDIEEINKGIFVEDPTK
DINPILLKHVEELNLTARSSNCLKAVNIRLIGELVQKTENELLKAPNFGKKSLTEIKDKLSELGLSLGTLIENWPQDL
>Mature_317_residues
ALENLLHPTNIKIDEYAKNATKFSFEALERGVGYTLGFALKQTMLYSIAGACVTSIKINDGKVTSLEDVIPCDETVADII
LNVKSLPVTLAEGVETGTITFELSGSEEEIFSEEAKLSEGLAITEEVFICSYNGGKKLKIEAKVEKGVGFRPAQDNFKDG
EFLLDATFSPVVFCDFEIKDARVGRRTDLDKLELNIKTNGNVNCEEALRLAATKIQNQLRNIVDIEEINKGIFVEDPTKD
INPILLKHVEELNLTARSSNCLKAVNIRLIGELVQKTENELLKAPNFGKKSLTEIKDKLSELGLSLGTLIENWPQDL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=318, Percent_Identity=38.9937106918239, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 35128; Mature: 34996

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALENLLHPTNIKIDEYAKNATKFSFEALERGVGYTLGFALKQTMLYSIAGACVTSIKIN
CCHHHCCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
DGKVTSLEDVIPCDETVADIILNVKSLPVTLAEGVETGTITFELSGSEEEIFSEEAKLSE
CCCCCCHHHCCCCHHHHHHHHHHHHCCCEEHHCCCCCCEEEEEECCCHHHHHHHHHHHHC
GLAITEEVFICSYNGGKKLKIEAKVEKGVGFRPAQDNFKDGEFLLDATFSPVVFCDFEIK
CCEEEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEC
DARVGRRTDLDKLELNIKTNGNVNCEEALRLAATKIQNQLRNIVDIEEINKGIFVEDPTK
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEECCCC
DINPILLKHVEELNLTARSSNCLKAVNIRLIGELVQKTENELLKAPNFGKKSLTEIKDKL
CCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SELGLSLGTLIENWPQDL
HHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
ALENLLHPTNIKIDEYAKNATKFSFEALERGVGYTLGFALKQTMLYSIAGACVTSIKIN
CHHHCCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEC
DGKVTSLEDVIPCDETVADIILNVKSLPVTLAEGVETGTITFELSGSEEEIFSEEAKLSE
CCCCCCHHHCCCCHHHHHHHHHHHHCCCEEHHCCCCCCEEEEEECCCHHHHHHHHHHHHC
GLAITEEVFICSYNGGKKLKIEAKVEKGVGFRPAQDNFKDGEFLLDATFSPVVFCDFEIK
CCEEEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEC
DARVGRRTDLDKLELNIKTNGNVNCEEALRLAATKIQNQLRNIVDIEEINKGIFVEDPTK
CCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEECCCC
DINPILLKHVEELNLTARSSNCLKAVNIRLIGELVQKTENELLKAPNFGKKSLTEIKDKL
CCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SELGLSLGTLIENWPQDL
HHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA