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The map label for this gene is gadA [H]

Identifier: 134301225

GI number: 134301225

Start: 85585

End: 86931

Strand: Direct

Name: gadA [H]

Synonym: FTW_0076

Alternate gene names: 134301225

Gene position: 85585-86931 (Clockwise)

Preceding gene: 134301224

Following gene: 134301226

Centisome position: 4.51

GC content: 37.56

Gene sequence:

>1347_bases
ATGGCATTACATGGTAAAAAAGATACAATTGATAACCTTGATTTTTTTGAACAATCTTTACCAAAGTTTAAACTTCCACT
AAATTCACAAGATCCTTTAGAAGTGTATCAAGAAATCAAAGATGAGTTAATGCTAGATGGTAATTCGAAGCAAAATTTAG
CGACGTTTTGCCAAACGGAAGTTGATGATTTCATTCACAAGCTTATGGATGATTGCATTGATAAAAATATGATTGACAAA
GATGAATATCCGCAGACTGCGGAAATAGAGTCTCGCTGTGTAAATATCCTTGCAAACCTATGGAACTCTTCAGCAGAAAA
TGCAATTGGCTGCTCAACGACTGGCTCATCAGAGGCTGCTATGCTTGGCGGTATGGCAATGAAGTGGCGTTGGCGAGATA
AAATGAAAGCGCAAGGTAAAGATTATACAAAACCTAATCTTGTTACAGGACCTGTGCAAGTGTGCTGGCATAAATTTGCT
AGATATTGGGATATTGAGCTTAGAGAAATTCCAATGTCGAATGAAAGTTTAATAATGACTCCAGAGGCTGTGCTTGAAAG
ATGTGATGAAAATACAATCGGTGTGGTGCCAACTCTTGGAGTGACTTTTACAGGGCAGTATGAGCCAGTAGAACAGGTAT
GTAAAGCTCTTGATGATTTTGAACGACAAACAGGCGTAGATATTCCAGTTCATGGTGATGCAGCATCTGGAGGTTTTTTG
GCACCATTTGTAGAGCCTGAACTTAAGTGGGACTTTAGATTACCAAGAGTTAAGTCAATTAACTCATCAGGACATAAGTT
TGGTTTATCGCCACTTGGGGTTGGTTGGGTTATTTGGGCTGATAAAAAGTACTTACCAGATGATTTGATTTTTAATGTTA
ATTACCTAGGTGGTAATATGCCAACATTTGCACTGAATTTCTCGCGACCTGGTGGTCAGATAGTAGCCCAGTATTATAAT
TTTGTTAGGTTGGGTTTTGAAGGCTATAAGAAAGTTCATCAGCTTTGCTATGATGTTGCAGAATATATTGCTAAAGAGCT
TAGAAAAATGGAAATTTTTGAAATTATTCATGCTGGAGAGGGTGGTATTCCTGCGGTTAGCTGGTCACTTAAAGCAACTA
AAGAGTATAGTTTGTTTGATATATCTGAGAAAGTTCGCGCTAAAGGTTGGCAAATAGCAGCTTACACAATGCCAAACAAT
CGTGAAGATTTAGTGGTTATGCGTGTATTAGTTAGAAGAGGCTTTAGTTATGACTTGGCACAGTTGATGATTAGAGATTT
AGTTGCGGTGATTGATTCATTAGAGGGAAAGTTAAAAATATTAAAAAGGAGTAGTTTTGCACATTAG

Upstream 100 bases:

>100_bases
ATAGTTTCGATATGTTGTAAAAATATTGTCGCTCAGAAAATTAATCTATTCGAGATAGTAGATTTTAAATTTAAACAGAT
ACAGCTTTAGAGAGAGGATT

Downstream 100 bases:

>100_bases
TATTTTAGGTGATTTTGATTAAGTTATCGCGTTAAATATTGTTATAATATTGTTTGTTAATAATTAATTTATACTAAGAG
TAGTCGGAAGTTTATGTGTG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLATFCQTEVDDFIHKLMDDCIDKNMIDK
DEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAAMLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFA
RYWDIELREIPMSNESLIMTPEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHGDAASGGFL
APFVEPELKWDFRLPRVKSINSSGHKFGLSPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQIVAQYYN
FVRLGFEGYKKVHQLCYDVAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKATKEYSLFDISEKVRAKGWQIAAYTMPNN
REDLVVMRVLVRRGFSYDLAQLMIRDLVAVIDSLEGKLKILKRSSFAH

Sequences:

>Translated_448_residues
MALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLATFCQTEVDDFIHKLMDDCIDKNMIDK
DEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAAMLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFA
RYWDIELREIPMSNESLIMTPEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHGDAASGGFL
APFVEPELKWDFRLPRVKSINSSGHKFGLSPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQIVAQYYN
FVRLGFEGYKKVHQLCYDVAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKATKEYSLFDISEKVRAKGWQIAAYTMPNN
REDLVVMRVLVRRGFSYDLAQLMIRDLVAVIDSLEGKLKILKRSSFAH
>Mature_447_residues
ALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLATFCQTEVDDFIHKLMDDCIDKNMIDKD
EYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAAMLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFAR
YWDIELREIPMSNESLIMTPEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHGDAASGGFLA
PFVEPELKWDFRLPRVKSINSSGHKFGLSPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQIVAQYYNF
VRLGFEGYKKVHQLCYDVAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKATKEYSLFDISEKVRAKGWQIAAYTMPNNR
EDLVVMRVLVRRGFSYDLAQLMIRDLVAVIDSLEGKLKILKRSSFAH

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=404, Percent_Identity=24.2574257425743, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787769, Length=435, Percent_Identity=57.0114942528736, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789934, Length=435, Percent_Identity=57.0114942528736, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17557272, Length=329, Percent_Identity=25.8358662613982, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25148342, Length=322, Percent_Identity=21.7391304347826, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543922, Length=392, Percent_Identity=22.1938775510204, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323905, Length=466, Percent_Identity=41.6309012875536, Blast_Score=329, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6320500, Length=354, Percent_Identity=24.0112994350282, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355963, Length=330, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24654344, Length=330, Percent_Identity=26.6666666666667, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 50788; Mature: 50657

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLATFCQTE
CCCCCCCCCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHH
VDDFIHKLMDDCIDKNMIDKDEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
MLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFARYWDIELREIPMSNESLIMT
HHCCEEEEEEHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCCEEEC
PEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHGDAASGGFL
HHHHHHHCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
APFVEPELKWDFRLPRVKSINSSGHKFGLSPLGVGWVIWADKKYLPDDLIFNVNYLGGNM
CCCCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCEEEEEECCCCCCCCCEEEEEECCCCC
PTFALNFSRPGGQIVAQYYNFVRLGFEGYKKVHQLCYDVAEYIAKELRKMEIFEIIHAGE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGIPAVSWSLKATKEYSLFDISEKVRAKGWQIAAYTMPNNREDLVVMRVLVRRGFSYDLA
CCCCEEEEEEECCCCCCEEEHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHH
QLMIRDLVAVIDSLEGKLKILKRSSFAH
HHHHHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
ALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLATFCQTE
CCCCCCCCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHH
VDDFIHKLMDDCIDKNMIDKDEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
MLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFARYWDIELREIPMSNESLIMT
HHCCEEEEEEHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCCEEEC
PEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHGDAASGGFL
HHHHHHHCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
APFVEPELKWDFRLPRVKSINSSGHKFGLSPLGVGWVIWADKKYLPDDLIFNVNYLGGNM
CCCCCCCCCCCCCCCCHHCCCCCCCEECCCCCCCEEEEEECCCCCCCCCEEEEEECCCCC
PTFALNFSRPGGQIVAQYYNFVRLGFEGYKKVHQLCYDVAEYIAKELRKMEIFEIIHAGE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGIPAVSWSLKATKEYSLFDISEKVRAKGWQIAAYTMPNNREDLVVMRVLVRRGFSYDLA
CCCCEEEEEEECCCCCCEEEHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHH
QLMIRDLVAVIDSLEGKLKILKRSSFAH
HHHHHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]