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The map label for this gene is purF [H]

Identifier: 134301226

GI number: 134301226

Start: 87025

End: 88515

Strand: Direct

Name: purF [H]

Synonym: FTW_0077

Alternate gene names: 134301226

Gene position: 87025-88515 (Clockwise)

Preceding gene: 134301225

Following gene: 134301227

Centisome position: 4.58

GC content: 36.89

Gene sequence:

>1491_bases
ATGTGTGGAGTAATTGGAGTCGCTGGACCAGATCAGGTTAGTTATGCATTATTTTATGGTTTAAGTCTTTTGCAGCATAG
GGGTCAAGATGCTGCAGGGATTGCAACTATGGATCATGGGCACTTTTTTATCCGTAAAAATACAGGTCTAGTAAGTGATG
TATTTACAGATGAAAAACTAGAGAAGTCAAAAGGTAATATGGGTATCGGTCATGTTAGATATCCTACTGCAGGTAGCTTG
GGTGCTGCTGATAGCCAACCTTTTTATGTAAATAATCCCCATGGTATAGTTTTTGTTCATAATGGCAATCTGACTAATGT
GCCAGAGCTTGCACAAATGCTCCATGATATTGAAAGACGCCATCTTAATACAAGTTCAGATTCCGAATTATTGCTTAATT
TCTTTGCTTGTGGGATGAATAAATCAAAAGGATCTGCGACTTCTGAGGCTGTTTATAAAGCATGTAAATTTGTTTTTGAG
CATGTCAAAGGTGGTTATGCATGTACTGCGATGATAGCAAATTTTGGTTTAATTGCTTTTAGAGATCCTTATGGTATTCG
TCCACTTATGCTTGGTTTTAAAGAGTACGATGATGGTGAAAAAGCTTACATGGTCGCTAGTGAAAGTGTTGCTTTAGATA
TTTCCGGATTTAAAGTATTACGTGATGTTGAGCCTGGTGAGGTGATAATAATTACAGAGGATAGAAAAGTTCACTCTAAA
ATCTGTGCAAAGAATCCAGTTTTGGCACCATGCCTATTTGAGTATGTATACTTTGCACGTCCTGATAGTATTATGAATGG
TGTGAGTGTTTATCAAGCGCGAGTAGATGCGGGTAAAATCCTAAGTAAGAGAATCAAAGAAGCGTGGAAGGATAAGGATA
TCGATATCGTTATCCCTGTACCTGAGACAGGTAGAGCCTCTGCTCAAGAGATTGCAACAGCATTAGGTGTTGAGTACCGT
GAAGGTTTTGTTAAGAATCGCTATGTTGGTAGAACTTTTATCATGCCAGAGAATGTTGATAGAAAGAATTTTGTCAGAAG
AAAACTAAACCCTATTCCAGCAGAATTTAGGGATAAAAATGTTCTACTTGTTGATGATTCTATTGTCCGAGGTACAACAT
CAAAGCGTATTATTGAGATGATTAGAGATTTAGGTGCTAAATCTGTTTATCTTGCTTCAGTATCTCCAGCCGTTCGTTAT
CCAAATGTTTATGGTATTGATATGCCGGTTAAGTCGGATTTAATTGCTCATGGAAAGACTATAGAAGAGATTCGCCAGTG
GATTGGAGTAGATGGACTAATATATTTACCATTAGAGGATCTAAAAGAGATTATTCAAAAGCAAAACCCTAAAATTAGAG
AGTTTGAGGATAGTGTTTTCTCTGGTAATTACATTACAGGTGATGTCGATGATGCTTATCTAGATGCGCTAGAGAAACAT
CGTAAAGAGTTAAAAGAATTAGAAAAAAAATACAAAGGGTTTGATAACTAA

Upstream 100 bases:

>100_bases
ACATTAGTATTTTAGGTGATTTTGATTAAGTTATCGCGTTAAATATTGTTATAATATTGTTTGTTAATAATTAATTTATA
CTAAGAGTAGTCGGAAGTTT

Downstream 100 bases:

>100_bases
TGATTACGATTTTTGAAGGTTTAAGTGCACTGTCTCCTTTTAAGCGAGAAAAAATTTTAGCAGCTGCTAAAAAAATATCC
AATAAAGTTGAATCAGTATC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDHGHFFIRKNTGLVSDVFTDEKLEKSKGNMGIGHVRYPTAGSL
GAADSQPFYVNNPHGIVFVHNGNLTNVPELAQMLHDIERRHLNTSSDSELLLNFFACGMNKSKGSATSEAVYKACKFVFE
HVKGGYACTAMIANFGLIAFRDPYGIRPLMLGFKEYDDGEKAYMVASESVALDISGFKVLRDVEPGEVIIITEDRKVHSK
ICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKILSKRIKEAWKDKDIDIVIPVPETGRASAQEIATALGVEYR
EGFVKNRYVGRTFIMPENVDRKNFVRRKLNPIPAEFRDKNVLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRY
PNVYGIDMPVKSDLIAHGKTIEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKH
RKELKELEKKYKGFDN

Sequences:

>Translated_496_residues
MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDHGHFFIRKNTGLVSDVFTDEKLEKSKGNMGIGHVRYPTAGSL
GAADSQPFYVNNPHGIVFVHNGNLTNVPELAQMLHDIERRHLNTSSDSELLLNFFACGMNKSKGSATSEAVYKACKFVFE
HVKGGYACTAMIANFGLIAFRDPYGIRPLMLGFKEYDDGEKAYMVASESVALDISGFKVLRDVEPGEVIIITEDRKVHSK
ICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKILSKRIKEAWKDKDIDIVIPVPETGRASAQEIATALGVEYR
EGFVKNRYVGRTFIMPENVDRKNFVRRKLNPIPAEFRDKNVLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRY
PNVYGIDMPVKSDLIAHGKTIEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKH
RKELKELEKKYKGFDN
>Mature_496_residues
MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDHGHFFIRKNTGLVSDVFTDEKLEKSKGNMGIGHVRYPTAGSL
GAADSQPFYVNNPHGIVFVHNGNLTNVPELAQMLHDIERRHLNTSSDSELLLNFFACGMNKSKGSATSEAVYKACKFVFE
HVKGGYACTAMIANFGLIAFRDPYGIRPLMLGFKEYDDGEKAYMVASESVALDISGFKVLRDVEPGEVIIITEDRKVHSK
ICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKILSKRIKEAWKDKDIDIVIPVPETGRASAQEIATALGVEYR
EGFVKNRYVGRTFIMPENVDRKNFVRRKLNPIPAEFRDKNVLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRY
PNVYGIDMPVKSDLIAHGKTIEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKH
RKELKELEKKYKGFDN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=473, Percent_Identity=36.7864693446089, Blast_Score=252, Evalue=6e-67,
Organism=Escherichia coli, GI1788651, Length=485, Percent_Identity=57.1134020618557, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1790167, Length=215, Percent_Identity=26.9767441860465, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=468, Percent_Identity=33.974358974359, Blast_Score=228, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=492, Percent_Identity=49.390243902439, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI28573187, Length=470, Percent_Identity=35.3191489361702, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=34.9494949494949, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24659604, Length=474, Percent_Identity=35.0210970464135, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55382; Mature: 55382

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDHGHFFIRKNTGLVSDVFTDEKL
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCHHHHHCHHHH
EKSKGNMGIGHVRYPTAGSLGAADSQPFYVNNPHGIVFVHNGNLTNVPELAQMLHDIERR
HHCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHH
HLNTSSDSELLLNFFACGMNKSKGSATSEAVYKACKFVFEHVKGGYACTAMIANFGLIAF
HCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEE
RDPYGIRPLMLGFKEYDDGEKAYMVASESVALDISGFKVLRDVEPGEVIIITEDRKVHSK
ECCCCCCHHHHCCHHCCCCCEEEEEECCCEEEEECCHHEEECCCCCEEEEEECCCHHHHH
ICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKILSKRIKEAWKDKDIDIVIPV
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PETGRASAQEIATALGVEYREGFVKNRYVGRTFIMPENVDRKNFVRRKLNPIPAEFRDKN
CCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCE
VLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRYPNVYGIDMPVKSDLIAHGKT
EEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHCCCCEEECCCCCHHHHHHCCCH
IEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKH
HHHHHHHHCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHH
RKELKELEKKYKGFDN
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCGVIGVAGPDQVSYALFYGLSLLQHRGQDAAGIATMDHGHFFIRKNTGLVSDVFTDEKL
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCHHHHHCHHHH
EKSKGNMGIGHVRYPTAGSLGAADSQPFYVNNPHGIVFVHNGNLTNVPELAQMLHDIERR
HHCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHH
HLNTSSDSELLLNFFACGMNKSKGSATSEAVYKACKFVFEHVKGGYACTAMIANFGLIAF
HCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEE
RDPYGIRPLMLGFKEYDDGEKAYMVASESVALDISGFKVLRDVEPGEVIIITEDRKVHSK
ECCCCCCHHHHCCHHCCCCCEEEEEECCCEEEEECCHHEEECCCCCEEEEEECCCHHHHH
ICAKNPVLAPCLFEYVYFARPDSIMNGVSVYQARVDAGKILSKRIKEAWKDKDIDIVIPV
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PETGRASAQEIATALGVEYREGFVKNRYVGRTFIMPENVDRKNFVRRKLNPIPAEFRDKN
CCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCE
VLLVDDSIVRGTTSKRIIEMIRDLGAKSVYLASVSPAVRYPNVYGIDMPVKSDLIAHGKT
EEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHCCCCEEECCCCCHHHHHHCCCH
IEEIRQWIGVDGLIYLPLEDLKEIIQKQNPKIREFEDSVFSGNYITGDVDDAYLDALEKH
HHHHHHHHCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHH
RKELKELEKKYKGFDN
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]