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The map label for this gene is gsp [H]

Identifier: 134301224

GI number: 134301224

Start: 84933

End: 85574

Strand: Direct

Name: gsp [H]

Synonym: FTW_0075

Alternate gene names: 134301224

Gene position: 84933-85574 (Clockwise)

Preceding gene: 134301223

Following gene: 134301225

Centisome position: 4.47

GC content: 31.62

Gene sequence:

>642_bases
ATGCCACAAGTCTCAGCTTTTATTTTAGATTTTGAGAAATATAAAGCTGAGGACCTTAGAGGATATCTTTGTTGCAGAAA
TATTGATTCAGCAAGGTTTAATAATAAGCAAAAAATATTTTCACAGTTTATTCGCTATTTTGTTTTAGAAGAGTTTTATC
ATATTTCTTCACCAATTTTTTTAAATCAACATGGTAAACCGTACTTGGCAGATAACAGTGTTTTTTTCAATATTAGTCAT
ACACAGACAAAGCTTATAATTGCTGTTGCTGATCAAGAGATTGGTGTGGATATTGAAAAACTATCAGCTAGACGGAATAT
TATAAGAATCGCACAACGTTATTTTACTAGCTTAGAAAATCAATGGCTTGCTGCTAGTGATAATCCCGTCAAAGACTTTT
ATACGTTATGGACACTCAAGGAAGCTCAGGTAAAAAGAGACTCGCTTGGGATAGCTAAAGGCTTGAGTGGTGCTAATTTT
AGTAAAATCAATGACTCATGGCTTAGTAATAGTTATCCAGATGATTTTGTGACATTTTCATATGATGAATCAATAGTTTC
GATATGTTGTAAAAATATTGTCGCTCAGAAAATTAATCTATTCGAGATAGTAGATTTTAAATTTAAACAGATACAGCTTT
AG

Upstream 100 bases:

>100_bases
AATTACAGGGGAGTTTAACCTACAAAGACATTGAGTACATCAATACTATTTTAGGAACTACAGATATAATATCGCAGATT
GCAACGGAGTAATTAGTTTG

Downstream 100 bases:

>100_bases
AGAGAGGATTATGGCATTACATGGTAAAAAAGATACAATTGATAACCTTGATTTTTTTGAACAATCTTTACCAAAGTTTA
AACTTCCACTAAATTCACAA

Product: 4'-phosphopantetheinyl transferase family protein

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: Gramicidin synthase-activating enzyme [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MPQVSAFILDFEKYKAEDLRGYLCCRNIDSARFNNKQKIFSQFIRYFVLEEFYHISSPIFLNQHGKPYLADNSVFFNISH
TQTKLIIAVADQEIGVDIEKLSARRNIIRIAQRYFTSLENQWLAASDNPVKDFYTLWTLKEAQVKRDSLGIAKGLSGANF
SKINDSWLSNSYPDDFVTFSYDESIVSICCKNIVAQKINLFEIVDFKFKQIQL

Sequences:

>Translated_213_residues
MPQVSAFILDFEKYKAEDLRGYLCCRNIDSARFNNKQKIFSQFIRYFVLEEFYHISSPIFLNQHGKPYLADNSVFFNISH
TQTKLIIAVADQEIGVDIEKLSARRNIIRIAQRYFTSLENQWLAASDNPVKDFYTLWTLKEAQVKRDSLGIAKGLSGANF
SKINDSWLSNSYPDDFVTFSYDESIVSICCKNIVAQKINLFEIVDFKFKQIQL
>Mature_212_residues
PQVSAFILDFEKYKAEDLRGYLCCRNIDSARFNNKQKIFSQFIRYFVLEEFYHISSPIFLNQHGKPYLADNSVFFNISHT
QTKLIIAVADQEIGVDIEKLSARRNIIRIAQRYFTSLENQWLAASDNPVKDFYTLWTLKEAQVKRDSLGIAKGLSGANFS
KINDSWLSNSYPDDFVTFSYDESIVSICCKNIVAQKINLFEIVDFKFKQIQL

Specific function: Activates the five peptidyl carrier protein (PCP) domains of gramicidin synthase GrsAB, by transferring the 4'- phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for gramicidin S production [H]

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR004568 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 24731; Mature: 24600

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQVSAFILDFEKYKAEDLRGYLCCRNIDSARFNNKQKIFSQFIRYFVLEEFYHISSPIF
CCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEE
LNQHGKPYLADNSVFFNISHTQTKLIIAVADQEIGVDIEKLSARRNIIRIAQRYFTSLEN
ECCCCCCEEECCEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
QWLAASDNPVKDFYTLWTLKEAQVKRDSLGIAKGLSGANFSKINDSWLSNSYPDDFVTFS
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEE
YDESIVSICCKNIVAQKINLFEIVDFKFKQIQL
CHHHHHHHHHHHHHHHHCCHHHEECCHHHEECC
>Mature Secondary Structure 
PQVSAFILDFEKYKAEDLRGYLCCRNIDSARFNNKQKIFSQFIRYFVLEEFYHISSPIF
CCHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEE
LNQHGKPYLADNSVFFNISHTQTKLIIAVADQEIGVDIEKLSARRNIIRIAQRYFTSLEN
ECCCCCCEEECCEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
QWLAASDNPVKDFYTLWTLKEAQVKRDSLGIAKGLSGANFSKINDSWLSNSYPDDFVTFS
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEE
YDESIVSICCKNIVAQKINLFEIVDFKFKQIQL
CHHHHHHHHHHHHHHHHCCHHHEECCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7512553; 2477357; 8939709 [H]