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The map label for this gene is tolC [H]

Identifier: 134301223

GI number: 134301223

Start: 83395

End: 84924

Strand: Direct

Name: tolC [H]

Synonym: FTW_0074

Alternate gene names: 134301223

Gene position: 83395-84924 (Clockwise)

Preceding gene: 134301222

Following gene: 134301224

Centisome position: 4.39

GC content: 36.01

Gene sequence:

>1530_bases
ATGAAGAAGTTAACATTATATCTTTTAGGTTTTTTGGGGTTTTGTCTAGCTTTAGCTAATGAAGATACGCCTGTTCCTGA
ATATAAGTATGCAGGAAGACCTATTGGTACTGATGTTGCTGAAAATCCATATAGTGTCGAAAAACAATATGCCAAAGCAG
ATCAAATTAAGGTAGACAATAAATTCTATAATCTTAAAAAAGCTGATGAAGTCGCGATGGTTGGCGATACTGAGGACAAG
TATTATAGTTTAATTGATATCTATAAGTTGGCTGCTGAACATAATGCCGACTACCAAGCAGCAAGGTCTACTTTTGCGGC
AAATGTTGAAACTGTGCCGACTGCTTTAGGTGCATTATTACCACAGATTGATTTTAACTATAATCTAAGAAGAGATTTGT
ACAATCAGTTTGGTGGTAGAGTCAATGACACTTCAAATGTAGTAAATTTTAGTGGTTCTCAGGTTCTTTTTGATTGGAGT
AAATGGAAGACATATACTCAAGCAACCTATTTGCAAAAATCGTATGCGATGATTTATGCTAAAGCTGAACAAACACTTAT
AACTGATACTGTTACTGCATATTTTGAGCTGCTAAGAGCAGAACAGGCGTTACAGTTCCAATTTGCAAATGAGGCTTGGA
ACAAAAAGTTATATCTGACTCAGAAATATCAGTATAATTCGGGCATGGTTTCATATGCTGATTTTAAAACTACAGATGCA
CAATATCGTCAAGCAATCGCTGATAGGGTTAATGCACAAAGAAACTTCATTAATGCCAAAGCTGTGATGGCTAAACTTAT
CGGTAAGCGTATAAGCTCAATTTTATATATATCAAAAGATACTGAATTTGGTAATCCTGTTCCAGATGATATTAACTATT
GGTTGAATACAGCTGAGAAATATAATCTTGATATAGCGCAGAAAAAGTTTGAATATGAAGCAGCACAAGAAGGTGTTGGT
ATTCAATGGGGTAAATTTTTTCCTCAAGCTAATTTAACTGGCGGTATAATGATGTCACGTAACGCTCTTTCGGGTTCACC
AGCACAAATAGCCGAAATTCCAACCAAATATGACGTTGCTAATATCGGTGGTCAAGTTAGCTGGAATTTATTAAGAGGTG
GTTCTGACTATGCGCAACTCAAGCAAGCTAGTTATGATAATCAAGCGGCGAACTATGCCTTGCTACAAACCAAGAGAGAA
GTCTACGCTGGTACAGTAGAGGCATATCAAACAGTTGTTCTTGATGTAGTTAGAATTGAGGCGTTTAGAAAGTCTGTGTA
TTCAGGTATAGCTTCTGTAAAAGCGATATTAGAAGGTTTTGAAGCAGGTACACAGACAATTGTTGACTTACTTAACCGTC
AAGCAATCCTTGTCCAAGCACAATTATCATTTGCAGATGCTATATTCAACTATGTTGAAGATTATGTAAGTTTAAAGCAA
TTACAGGGGAGTTTAACCTACAAAGACATTGAGTACATCAATACTATTTTAGGAACTACAGATATAATATCGCAGATTGC
AACGGAGTAA

Upstream 100 bases:

>100_bases
GAACAGCTATATGTGATAAAGCATACTTAATAACAAAAACTCCAAAGCAAACATACGAAAAGAGTTTTCTCTTTAATACA
TACAATAAGTAAAGAGTGCT

Downstream 100 bases:

>100_bases
TTAGTTTGATGCCACAAGTCTCAGCTTTTATTTTAGATTTTGAGAAATATAAAGCTGAGGACCTTAGAGGATATCTTTGT
TGCAGAAATATTGATTCAGC

Product: TolC family outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDNKFYNLKKADEVAMVGDTEDK
YYSLIDIYKLAAEHNADYQAARSTFAANVETVPTALGALLPQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWS
KWKTYTQATYLQKSYAMIYAKAEQTLITDTVTAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEKYNLDIAQKKFEYEAAQEGVG
IQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVANIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKRE
VYAGTVEAYQTVVLDVVRIEAFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATE

Sequences:

>Translated_509_residues
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDNKFYNLKKADEVAMVGDTEDK
YYSLIDIYKLAAEHNADYQAARSTFAANVETVPTALGALLPQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWS
KWKTYTQATYLQKSYAMIYAKAEQTLITDTVTAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEKYNLDIAQKKFEYEAAQEGVG
IQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVANIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKRE
VYAGTVEAYQTVVLDVVRIEAFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATE
>Mature_509_residues
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDNKFYNLKKADEVAMVGDTEDK
YYSLIDIYKLAAEHNADYQAARSTFAANVETVPTALGALLPQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWS
KWKTYTQATYLQKSYAMIYAKAEQTLITDTVTAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEKYNLDIAQKKFEYEAAQEGVG
IQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVANIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKRE
VYAGTVEAYQTVVLDVVRIEAFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
LQGSLTYKDIEYINTILGTTDIISQIATE

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=430, Percent_Identity=28.3720930232558, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57242; Mature: 57242

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEECC
KFYNLKKADEVAMVGDTEDKYYSLIDIYKLAAEHNADYQAARSTFAANVETVPTALGALL
HHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHH
PQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWSKWKTYTQATYLQKSYAMIYA
CCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHEEEE
KAEQTLITDTVTAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEECCCCCCHH
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHCCCHH
YNLDIAQKKFEYEAAQEGVGIQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVA
CCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCHH
NIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKREVYAGTVEAYQTVVLDVVRIE
HCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LQGSLTYKDIEYINTILGTTDIISQIATE
HHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLTLYLLGFLGFCLALANEDTPVPEYKYAGRPIGTDVAENPYSVEKQYAKADQIKVDN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEECC
KFYNLKKADEVAMVGDTEDKYYSLIDIYKLAAEHNADYQAARSTFAANVETVPTALGALL
HHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHH
PQIDFNYNLRRDLYNQFGGRVNDTSNVVNFSGSQVLFDWSKWKTYTQATYLQKSYAMIYA
CCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHEEEE
KAEQTLITDTVTAYFELLRAEQALQFQFANEAWNKKLYLTQKYQYNSGMVSYADFKTTDA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEECCCCCCHH
QYRQAIADRVNAQRNFINAKAVMAKLIGKRISSILYISKDTEFGNPVPDDINYWLNTAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHCCCHH
YNLDIAQKKFEYEAAQEGVGIQWGKFFPQANLTGGIMMSRNALSGSPAQIAEIPTKYDVA
CCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCHH
NIGGQVSWNLLRGGSDYAQLKQASYDNQAANYALLQTKREVYAGTVEAYQTVVLDVVRIE
HCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRKSVYSGIASVKAILEGFEAGTQTIVDLLNRQAILVQAQLSFADAIFNYVEDYVSLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LQGSLTYKDIEYINTILGTTDIISQIATE
HHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]