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The map label for this gene is leuC1 [H]

Identifier: 134300177

GI number: 134300177

Start: 2555674

End: 2556930

Strand: Reverse

Name: leuC1 [H]

Synonym: Dred_2338

Alternate gene names: 134300177

Gene position: 2556930-2555674 (Counterclockwise)

Preceding gene: 134300178

Following gene: 134300176

Centisome position: 70.87

GC content: 48.69

Gene sequence:

>1257_bases
TTGGGCAAAACTATTATTGAGAAGATATTATCTTCTCATAGCGGCCAGGATTGCCAGGCCAATGACATCGTGGTAGCCCA
GGTTGATTTTGTCATGGGACAGGACGGTACATCACCGCTGGCTATTCGGGCCTATGAAAATATGGACGGCAAGGAACTAT
TTGATCCAGAAAAGGTTGCCTTGGTTATTGACCATAGTTCTCCCAGCCCCCTAGAAGGGGTATCTGCTTTACATAAAATT
ATGCGTCAGTTTGCTAAGCAAAAGGGTTGTCACCTTTATGACATTGGGGAAGGGGTTTGTCACCAACTGATTCCCGAAAG
TGGTAAGGTTGGTCCGGGTAGTCTGGTCATTGGTGCAGACAGTCATACCTGTACCTACGGGGCATTGAATGCTTTTTCCA
CCGGGGTTGGCTCCACGGACCTAGCCGGAGGACTGATTTCCGGCAAAATGTGGTTCAAGGTACCGGAAACCATTAAATTT
GTCTGCAATGGTGTACTGCCCAGCGGAGTTTATGCCAAGGATTTAATTCTCTACTTAATTGGTCAGGTAACTGCCGATGG
TTGTACCTATATGGCAGCGGAATACACCGGTGAGGCCATTGCTGCGTTGTCCATGGAAGGCCGCTTCACCATATCCAACA
TGGCCATTGAAATGGGGGCCAAGGCTGGTTTAATGGAAGCCGATGATAAAACCTTTGCATGGCTGAAACAATACACAGAA
CGGCAATTCACAGCGGTTTCTGCCGATGCTAATGCCAATTACTATAAGGTACTGGAACTTGATGTTTCCAATCTGGAGCC
CCAAGTGGCTAAACCCCACCGGGTGGATAACGTATCTCCCCTATCAGAGGTAAAAGGACTTCCTATTCAACAGGCCTTTT
TAGGAACCTGTACTAACGGTCGTCTGGAGGATCTGCGGATTGCTGCTTCAATCCTAGCTGGTAAAAAGGTTCATCCAGAT
GTACGGTTTATTGTAGCACCTGCATCCCGTAAGATATATCTGGAAGCTATGGAAGAAGGAGTCATTCAGCGGTTGGTGGA
AGCCGGTGCTGCGGTTGTTACACCCGGTTGCGGTCCCTGTGTGGGTACTCACAATGGTGTTCCTTCAGACGGTGAAACCG
TCATTTCCACGGCAAACCGTAATTTCAAGGGGCGCATGGGCAACAGCAATGCAGAAATTTACCTGGCTTCTCCGGCAACG
GTGGCAGCCTCAGCATTGACAGGTGTCATTAGCGACCCCAGAGACTTCGTAAAGTAG

Upstream 100 bases:

>100_bases
TTGCCTAAAATACGTTCTGCATCAGTATCCTTAAAGCGATCCTTGTTTGATAAAGAATTGGTTTATATCTATGAAGATCA
TTTCGGAAAGAGGGATTAAT

Downstream 100 bases:

>100_bases
GTGAGGCCGATGAAATTTCAAGGAAAAAGCCATAAGTTTGATAATGATGTTAATACCGATTATATAATATCGGGTAAATA
TAAGTTTAAAACATTGGATA

Product: homoaconitate hydratase family protein

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGKTIIEKILSSHSGQDCQANDIVVAQVDFVMGQDGTSPLAIRAYENMDGKELFDPEKVALVIDHSSPSPLEGVSALHKI
MRQFAKQKGCHLYDIGEGVCHQLIPESGKVGPGSLVIGADSHTCTYGALNAFSTGVGSTDLAGGLISGKMWFKVPETIKF
VCNGVLPSGVYAKDLILYLIGQVTADGCTYMAAEYTGEAIAALSMEGRFTISNMAIEMGAKAGLMEADDKTFAWLKQYTE
RQFTAVSADANANYYKVLELDVSNLEPQVAKPHRVDNVSPLSEVKGLPIQQAFLGTCTNGRLEDLRIAASILAGKKVHPD
VRFIVAPASRKIYLEAMEEGVIQRLVEAGAAVVTPGCGPCVGTHNGVPSDGETVISTANRNFKGRMGNSNAEIYLASPAT
VAASALTGVISDPRDFVK

Sequences:

>Translated_418_residues
MGKTIIEKILSSHSGQDCQANDIVVAQVDFVMGQDGTSPLAIRAYENMDGKELFDPEKVALVIDHSSPSPLEGVSALHKI
MRQFAKQKGCHLYDIGEGVCHQLIPESGKVGPGSLVIGADSHTCTYGALNAFSTGVGSTDLAGGLISGKMWFKVPETIKF
VCNGVLPSGVYAKDLILYLIGQVTADGCTYMAAEYTGEAIAALSMEGRFTISNMAIEMGAKAGLMEADDKTFAWLKQYTE
RQFTAVSADANANYYKVLELDVSNLEPQVAKPHRVDNVSPLSEVKGLPIQQAFLGTCTNGRLEDLRIAASILAGKKVHPD
VRFIVAPASRKIYLEAMEEGVIQRLVEAGAAVVTPGCGPCVGTHNGVPSDGETVISTANRNFKGRMGNSNAEIYLASPAT
VAASALTGVISDPRDFVK
>Mature_417_residues
GKTIIEKILSSHSGQDCQANDIVVAQVDFVMGQDGTSPLAIRAYENMDGKELFDPEKVALVIDHSSPSPLEGVSALHKIM
RQFAKQKGCHLYDIGEGVCHQLIPESGKVGPGSLVIGADSHTCTYGALNAFSTGVGSTDLAGGLISGKMWFKVPETIKFV
CNGVLPSGVYAKDLILYLIGQVTADGCTYMAAEYTGEAIAALSMEGRFTISNMAIEMGAKAGLMEADDKTFAWLKQYTER
QFTAVSADANANYYKVLELDVSNLEPQVAKPHRVDNVSPLSEVKGLPIQQAFLGTCTNGRLEDLRIAASILAGKKVHPDV
RFIVAPASRKIYLEAMEEGVIQRLVEAGAAVVTPGCGPCVGTHNGVPSDGETVISTANRNFKGRMGNSNAEIYLASPATV
AASALTGVISDPRDFVK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=347, Percent_Identity=30.2593659942363, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI8659555, Length=395, Percent_Identity=25.3164556962025, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786259, Length=474, Percent_Identity=32.4894514767932, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=25.9259259259259, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787531, Length=387, Percent_Identity=26.0981912144703, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI2367097, Length=381, Percent_Identity=25.9842519685039, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25149337, Length=345, Percent_Identity=28.1159420289855, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=345, Percent_Identity=28.1159420289855, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=26.7605633802817, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17568399, Length=523, Percent_Identity=24.8565965583174, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=33.3333333333333, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321429, Length=478, Percent_Identity=29.9163179916318, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6322261, Length=455, Percent_Identity=28.3516483516484, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323335, Length=446, Percent_Identity=29.5964125560538, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI281365315, Length=446, Percent_Identity=28.47533632287, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17864292, Length=446, Percent_Identity=28.47533632287, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=28.4671532846715, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571643, Length=447, Percent_Identity=29.082774049217, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24645686, Length=464, Percent_Identity=26.2931034482759, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137564, Length=404, Percent_Identity=25.990099009901, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44378; Mature: 44247

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTIIEKILSSHSGQDCQANDIVVAQVDFVMGQDGTSPLAIRAYENMDGKELFDPEKVA
CCHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEE
LVIDHSSPSPLEGVSALHKIMRQFAKQKGCHLYDIGEGVCHQLIPESGKVGPGSLVIGAD
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCCCCCCCEEEECC
SHTCTYGALNAFSTGVGSTDLAGGLISGKMWFKVPETIKFVCNGVLPSGVYAKDLILYLI
CCEEEHHCHHHHHCCCCCCHHCCCEECCEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHH
GQVTADGCTYMAAEYTGEAIAALSMEGRFTISNMAIEMGAKAGLMEADDKTFAWLKQYTE
HHHCCCCEEEEEHHHCCCEEEEEECCCCEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RQFTAVSADANANYYKVLELDVSNLEPQVAKPHRVDNVSPLSEVKGLPIQQAFLGTCTNG
HHEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCC
RLEDLRIAASILAGKKVHPDVRFIVAPASRKIYLEAMEEGVIQRLVEAGAAVVTPGCGPC
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHCCCEEECCCCCCC
VGTHNGVPSDGETVISTANRNFKGRMGNSNAEIYLASPATVAASALTGVISDPRDFVK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
GKTIIEKILSSHSGQDCQANDIVVAQVDFVMGQDGTSPLAIRAYENMDGKELFDPEKVA
CHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEE
LVIDHSSPSPLEGVSALHKIMRQFAKQKGCHLYDIGEGVCHQLIPESGKVGPGSLVIGAD
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCCCCCCCEEEECC
SHTCTYGALNAFSTGVGSTDLAGGLISGKMWFKVPETIKFVCNGVLPSGVYAKDLILYLI
CCEEEHHCHHHHHCCCCCCHHCCCEECCEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHH
GQVTADGCTYMAAEYTGEAIAALSMEGRFTISNMAIEMGAKAGLMEADDKTFAWLKQYTE
HHHCCCCEEEEEHHHCCCEEEEEECCCCEEEEHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RQFTAVSADANANYYKVLELDVSNLEPQVAKPHRVDNVSPLSEVKGLPIQQAFLGTCTNG
HHEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCC
RLEDLRIAASILAGKKVHPDVRFIVAPASRKIYLEAMEEGVIQRLVEAGAAVVTPGCGPC
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHCCCEEECCCCCCC
VGTHNGVPSDGETVISTANRNFKGRMGNSNAEIYLASPATVAASALTGVISDPRDFVK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]