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The map label for this gene is leuD2 [H]

Identifier: 134300176

GI number: 134300176

Start: 2555164

End: 2555664

Strand: Reverse

Name: leuD2 [H]

Synonym: Dred_2337

Alternate gene names: 134300176

Gene position: 2555664-2555164 (Counterclockwise)

Preceding gene: 134300177

Following gene: 134300175

Centisome position: 70.83

GC content: 38.32

Gene sequence:

>501_bases
ATGAAATTTCAAGGAAAAAGCCATAAGTTTGATAATGATGTTAATACCGATTATATAATATCGGGTAAATATAAGTTTAA
AACATTGGATATGAATGAACTGGCAAAACATGTTATGGAAGATTTGGACCCTGATTTTTACAGCAAGGTTACTCCCGGAG
ATTTTATTGTTGCCGGCAGTAATTTTGGCTGTGGTTCCTCTAGGGAACAGGCACCTCTGGCCATTAAACATGCTAATATC
AGTGCTGTTTTAGCCAAGTCCTTTGCCCGGATTTTTTATCGCAATGCCATTAATACTGGTTTGCCGGTGCTGGAATGTGA
TACCGATTTAATTGATCCCGGTGATTTACTGGATGTGGATCTAACAAACGGTGTTATCATTAACCGTACTAAAGATTTAA
AAATTTCTGTAAAGCCACTGCCCCAGGTGATGATTAAAATTCTAAATGATGGGGGATTGGCACCGCACTTTCGAAAATAC
GGTGGTTTCAACTTTGGTTAG

Upstream 100 bases:

>100_bases
CAATGCAGAAATTTACCTGGCTTCTCCGGCAACGGTGGCAGCCTCAGCATTGACAGGTGTCATTAGCGACCCCAGAGACT
TCGTAAAGTAGGTGAGGCCG

Downstream 100 bases:

>100_bases
AAGAAAAAGTATCCACCGGAGTATAAGGGGTTATTATTAAACAACCTAAAATCAACAAACTCAAAACCACCTGCAAAGGT
GGTTTTGAGTTTGTAAAAAA

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKFQGKSHKFDNDVNTDYIISGKYKFKTLDMNELAKHVMEDLDPDFYSKVTPGDFIVAGSNFGCGSSREQAPLAIKHANI
SAVLAKSFARIFYRNAINTGLPVLECDTDLIDPGDLLDVDLTNGVIINRTKDLKISVKPLPQVMIKILNDGGLAPHFRKY
GGFNFG

Sequences:

>Translated_166_residues
MKFQGKSHKFDNDVNTDYIISGKYKFKTLDMNELAKHVMEDLDPDFYSKVTPGDFIVAGSNFGCGSSREQAPLAIKHANI
SAVLAKSFARIFYRNAINTGLPVLECDTDLIDPGDLLDVDLTNGVIINRTKDLKISVKPLPQVMIKILNDGGLAPHFRKY
GGFNFG
>Mature_166_residues
MKFQGKSHKFDNDVNTDYIISGKYKFKTLDMNELAKHVMEDLDPDFYSKVTPGDFIVAGSNFGCGSSREQAPLAIKHANI
SAVLAKSFARIFYRNAINTGLPVLECDTDLIDPGDLLDVDLTNGVIINRTKDLKISVKPLPQVMIKILNDGGLAPHFRKY
GGFNFG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=95, Percent_Identity=45.2631578947368, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=152, Percent_Identity=32.8947368421053, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18371; Mature: 18371

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQGKSHKFDNDVNTDYIISGKYKFKTLDMNELAKHVMEDLDPDFYSKVTPGDFIVAGS
CCCCCCCCCCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCCCHHHCCCCCCEEEECC
NFGCGSSREQAPLAIKHANISAVLAKSFARIFYRNAINTGLPVLECDTDLIDPGDLLDVD
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
LTNGVIINRTKDLKISVKPLPQVMIKILNDGGLAPHFRKYGGFNFG
CCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKFQGKSHKFDNDVNTDYIISGKYKFKTLDMNELAKHVMEDLDPDFYSKVTPGDFIVAGS
CCCCCCCCCCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCCCCHHHCCCCCCEEEECC
NFGCGSSREQAPLAIKHANISAVLAKSFARIFYRNAINTGLPVLECDTDLIDPGDLLDVD
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
LTNGVIINRTKDLKISVKPLPQVMIKILNDGGLAPHFRKYGGFNFG
CCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]