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The map label for this gene is lutR [H]

Identifier: 134300032

GI number: 134300032

Start: 2391137

End: 2391859

Strand: Reverse

Name: lutR [H]

Synonym: Dred_2188

Alternate gene names: 134300032

Gene position: 2391859-2391137 (Counterclockwise)

Preceding gene: 134300033

Following gene: 134300031

Centisome position: 66.29

GC content: 35.96

Gene sequence:

>723_bases
ATGGAGTTTAAGCCTATAAAAGCAAAAAAAGTTTATGAAGAAATTGTGCAACAAATAAAAGAGATGATTAGCAAGGGAGA
ACTTCAAGCAGGGGATAAAATGATTCCAGAGAGGGAGTTAGCGGAAAGACTTCAGGTAGGTCGTTCTGCTGTTAGAGAAG
CTTATCGTGCCCTGGAAGCCATTGGAATTATTGAGATAAGGTCTGGAGAGGGTACGTTTGTTCGCAAATTAGGAAACAAA
CCTATGACTGATATTATGTCATTCGCTGTTATCACTGGAAAAGATACTCTTTTTGAACTTTTGGAATTACGTAAAATTGT
AGAAACAGAGGCTGCTTCTCTAGCTGCACAGCGAAGAACAGAGGAAGATATAAATAATATCGAAAAATGGCTTAAGCAAA
TGAAGCTAGACATTGAAAAAGGAAATTTAGGTGATATTTCCGATATAAACTTTCATTATGCACTTACAGATGCTGCTCAT
AATTCGTTATTAACAAGACTGATGAATTCAATATCTGAAACCATGAAAAAAGAAATGGTAACAGTACGTTTAAATTTATA
TGAAGCCCATAGAACACCTTGGAAGCTCTATCATCAGCATGAAGTGATCACTGAGGCTATAAAAAAAGGAGATGCGGAAG
GGGCTCGCGCTGCAATGTTAGATCATTTAGTCAATGCTGAGAGAGAATTAATACAATATAAGAACAAAAGCAATAAAGTA
TAA

Upstream 100 bases:

>100_bases
CAGGGTGAAAACTTTATGACATGATCAGATAGAAATATATCTTTTAATATTTAATAAAGTTGCTTTGCAAAAATAAAATA
TATTATATGGAGTGCAAAAG

Downstream 100 bases:

>100_bases
GCTAAGGAAACTAGTTGGTTAGGTTTTTAGAAAATAATATAAAATTATGTTTACTTTATAGTTTTATTTTCACAAAGATT
TACAACAGTTAATAAATTAA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MEFKPIKAKKVYEEIVQQIKEMISKGELQAGDKMIPERELAERLQVGRSAVREAYRALEAIGIIEIRSGEGTFVRKLGNK
PMTDIMSFAVITGKDTLFELLELRKIVETEAASLAAQRRTEEDINNIEKWLKQMKLDIEKGNLGDISDINFHYALTDAAH
NSLLTRLMNSISETMKKEMVTVRLNLYEAHRTPWKLYHQHEVITEAIKKGDAEGARAAMLDHLVNAERELIQYKNKSNKV

Sequences:

>Translated_240_residues
MEFKPIKAKKVYEEIVQQIKEMISKGELQAGDKMIPERELAERLQVGRSAVREAYRALEAIGIIEIRSGEGTFVRKLGNK
PMTDIMSFAVITGKDTLFELLELRKIVETEAASLAAQRRTEEDINNIEKWLKQMKLDIEKGNLGDISDINFHYALTDAAH
NSLLTRLMNSISETMKKEMVTVRLNLYEAHRTPWKLYHQHEVITEAIKKGDAEGARAAMLDHLVNAERELIQYKNKSNKV
>Mature_240_residues
MEFKPIKAKKVYEEIVQQIKEMISKGELQAGDKMIPERELAERLQVGRSAVREAYRALEAIGIIEIRSGEGTFVRKLGNK
PMTDIMSFAVITGKDTLFELLELRKIVETEAASLAAQRRTEEDINNIEKWLKQMKLDIEKGNLGDISDINFHYALTDAAH
NSLLTRLMNSISETMKKEMVTVRLNLYEAHRTPWKLYHQHEVITEAIKKGDAEGARAAMLDHLVNAERELIQYKNKSNKV

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=233, Percent_Identity=31.3304721030043, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1786303, Length=234, Percent_Identity=33.7606837606838, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1790032, Length=226, Percent_Identity=29.2035398230088, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789353, Length=229, Percent_Identity=29.2576419213974, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI87082208, Length=222, Percent_Identity=28.8288288288288, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994955, Length=222, Percent_Identity=27.9279279279279, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789621, Length=245, Percent_Identity=26.1224489795918, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787821, Length=191, Percent_Identity=29.3193717277487, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27467; Mature: 27467

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKPIKAKKVYEEIVQQIKEMISKGELQAGDKMIPERELAERLQVGRSAVREAYRALEA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGIIEIRSGEGTFVRKLGNKPMTDIMSFAVITGKDTLFELLELRKIVETEAASLAAQRRT
HHHEEEECCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEDINNIEKWLKQMKLDIEKGNLGDISDINFHYALTDAAHNSLLTRLMNSISETMKKEMV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TVRLNLYEAHRTPWKLYHQHEVITEAIKKGDAEGARAAMLDHLVNAERELIQYKNKSNKV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEFKPIKAKKVYEEIVQQIKEMISKGELQAGDKMIPERELAERLQVGRSAVREAYRALEA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGIIEIRSGEGTFVRKLGNKPMTDIMSFAVITGKDTLFELLELRKIVETEAASLAAQRRT
HHHEEEECCCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEDINNIEKWLKQMKLDIEKGNLGDISDINFHYALTDAAHNSLLTRLMNSISETMKKEMV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TVRLNLYEAHRTPWKLYHQHEVITEAIKKGDAEGARAAMLDHLVNAERELIQYKNKSNKV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]