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The map label for this gene is lutR [H]

Identifier: 134300031

GI number: 134300031

Start: 2390270

End: 2390989

Strand: Reverse

Name: lutR [H]

Synonym: Dred_2187

Alternate gene names: 134300031

Gene position: 2390989-2390270 (Counterclockwise)

Preceding gene: 134300032

Following gene: 134300030

Centisome position: 66.27

GC content: 39.44

Gene sequence:

>720_bases
ATGGAATTTAAGCCTATAAAACCTAAAAAAATATACGAAGAAATTGTGGATCAGCTTAAAGGAATGATAACTAGGGGTGA
ACTTAGCCCAGGGGACAAACTCTTGCCAGAAAGGGAGCTAGCAGACCAACTTCAGGTTGGCCGTTCTGCTGTTCGTGAGG
CATATCGTGCCCTTGAAGCCATTGGCATTATAGAAATCAGAGCTGGTGAAGGAACCTTTGTGCGTGAATTAAATACAAAA
TCTATGACGGACATTATGTCCTTTGCGGTGATTACCGGAAAGGGTGCTATATTAGAACTTCTGGAACTTAGAAAAATTAT
TGAAATTGAGGCCGCTGCCCTTGCGGCAGAACGCTTGACAAAGGATAATTTGATTAACCTTAAAAAATGCTTGGACAAAA
TGAAAGAGGATGTCAATAAAGGGGTAGTAGGTGAAGTATCCGACTTAATATTTCACCATGCCATTACAGAAGCGGCGCAT
AATTCTCTACTTACGAGAGTTATTAGTTCTGTTTCTGATACGATGAAAACTGAGATGAAAAAGGTTCGTGAAAGAATGTA
TGAAACGCCTGGCAGACCTGAAATATTATATAAGCAGCATGAGGAAATTTACCAGGCCATTGTAAATAGAGATCCTGCAG
AAGCAAGAAAAGCCATGCTAAACCATTTGGTCAGTACAGAGCAGGTTTTGATCCAAGTAATGAATAAAGATAAGATTTAA

Upstream 100 bases:

>100_bases
TGTTTACTTTATAGTTTTATTTTCACAAAGATTTACAACAGTTAATAAATTAATTATTATTAGTGTAGAGTAATTTTTTA
CTAATTGGAGTTGTTAATTT

Downstream 100 bases:

>100_bases
CCCAGCAATGCTGGGTTTCTTGCTTTTTCAGTATTGGTTACCCTATAATTGAACGAAGAAAAGTTTGTACGGGGGAATTG
TATGGAGCTGCAAAAGAATA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEFKPIKPKKIYEEIVDQLKGMITRGELSPGDKLLPERELADQLQVGRSAVREAYRALEAIGIIEIRAGEGTFVRELNTK
SMTDIMSFAVITGKGAILELLELRKIIEIEAAALAAERLTKDNLINLKKCLDKMKEDVNKGVVGEVSDLIFHHAITEAAH
NSLLTRVISSVSDTMKTEMKKVRERMYETPGRPEILYKQHEEIYQAIVNRDPAEARKAMLNHLVSTEQVLIQVMNKDKI

Sequences:

>Translated_239_residues
MEFKPIKPKKIYEEIVDQLKGMITRGELSPGDKLLPERELADQLQVGRSAVREAYRALEAIGIIEIRAGEGTFVRELNTK
SMTDIMSFAVITGKGAILELLELRKIIEIEAAALAAERLTKDNLINLKKCLDKMKEDVNKGVVGEVSDLIFHHAITEAAH
NSLLTRVISSVSDTMKTEMKKVRERMYETPGRPEILYKQHEEIYQAIVNRDPAEARKAMLNHLVSTEQVLIQVMNKDKI
>Mature_239_residues
MEFKPIKPKKIYEEIVDQLKGMITRGELSPGDKLLPERELADQLQVGRSAVREAYRALEAIGIIEIRAGEGTFVRELNTK
SMTDIMSFAVITGKGAILELLELRKIIEIEAAALAAERLTKDNLINLKKCLDKMKEDVNKGVVGEVSDLIFHHAITEAAH
NSLLTRVISSVSDTMKTEMKKVRERMYETPGRPEILYKQHEEIYQAIVNRDPAEARKAMLNHLVSTEQVLIQVMNKDKI

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=235, Percent_Identity=29.7872340425532, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790032, Length=221, Percent_Identity=31.6742081447964, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1786303, Length=233, Percent_Identity=30.9012875536481, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789353, Length=231, Percent_Identity=29.8701298701299, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI87082208, Length=236, Percent_Identity=28.3898305084746, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789621, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994955, Length=225, Percent_Identity=25.7777777777778, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787821, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27023; Mature: 27023

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKPIKPKKIYEEIVDQLKGMITRGELSPGDKLLPERELADQLQVGRSAVREAYRALEA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGIIEIRAGEGTFVRELNTKSMTDIMSFAVITGKGAILELLELRKIIEIEAAALAAERLT
HHHEEEECCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KDNLINLKKCLDKMKEDVNKGVVGEVSDLIFHHAITEAAHNSLLTRVISSVSDTMKTEMK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVRERMYETPGRPEILYKQHEEIYQAIVNRDPAEARKAMLNHLVSTEQVLIQVMNKDKI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEFKPIKPKKIYEEIVDQLKGMITRGELSPGDKLLPERELADQLQVGRSAVREAYRALEA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGIIEIRAGEGTFVRELNTKSMTDIMSFAVITGKGAILELLELRKIIEIEAAALAAERLT
HHHEEEECCCCCHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KDNLINLKKCLDKMKEDVNKGVVGEVSDLIFHHAITEAAHNSLLTRVISSVSDTMKTEMK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVRERMYETPGRPEILYKQHEEIYQAIVNRDPAEARKAMLNHLVSTEQVLIQVMNKDKI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]