Click here to switch to the map view.

The map label for this gene is yfnG [H]

Identifier: 134299746

GI number: 134299746

Start: 2084660

End: 2085655

Strand: Reverse

Name: yfnG [H]

Synonym: Dred_1896

Alternate gene names: 134299746

Gene position: 2085655-2084660 (Counterclockwise)

Preceding gene: 134299747

Following gene: 134299745

Centisome position: 57.8

GC content: 45.78

Gene sequence:

>996_bases
ATGAATCTTTGGCAAAATAAAAATGTGCTGATAACCGGATGCTCAGGAATAATTGGCAGTTGGTTGACTCGCAGGCTAGT
TGATGAAGGGGCTAATGTGGTAGGGATTGTTCGGGATCGTTTGGGCTACGGAAATCTACTGCAGGAACAGACCATCAACA
GGATGGTTGTTGCCCATGGTGATATTACCGATTTTCTATTTATGAGCAGGGTTATGGCAGAGTATGAAATTGATACGGTG
TTTCATCTGGCTGCCCAAACCATTGTTACCATTGCCAACCGGTCACCTTTGTCAACTTTTGAATCAAATTTGAGAGGGAC
TTGGATAGTTCTAGAGGCCTGCCGACTGTCTCCCACCGTTGAAAGGGTTGTGGTTGCTTCCAGCGATAAGGCATATGGTG
ACAGTAAAGAGTTACCCTATAGAGAAGATCAACCTTTAAGGGGAAAACACCCCTATGATGTCTCTAAATCCTGCACCGAT
TTGCTTGCCCAGAGTTATTATCATACTTATAGACTACCTGTTGCCATAACCAGATTGGCCAATGTATACGGTGGGGGGGA
CTTGAATTTTAATCGCATCATTCCAGGGACCATCAAGGCGGTGCTGGAAGGTCGACCGCCTGTACTGCGTAGTGATGGCA
GCCCCCTCCGGGAATACCTCTATGTAGAAGATGCTGTGGATGCCTACCTAACTCTGGCAAAAAATCTGCATCGTTCCAAT
GTTATGGGTGAGGCCTTTAATTTTGCACCTCAGCAGCCCTATCAAGTACTGGAAATTGTGCAAGAAATAATAAGGATAGC
GGGTAAGGAGTTCGTGCCGGAAATTAGGAAACATATGAACGGGGAAATACTTCACCAATATAGTGATAGTAGCAAGGCCA
AAGAACGATTAAATTGGCATACCCGGTGGGACCTGACTAAGGGGCTGTCCGTTACCATCGAATGGTACCGCAAGTTTCTG
ATGGATCGAAGACCAGTCAAGTATACCATGGAGTGA

Upstream 100 bases:

>100_bases
AAACACAGAGGTTTTTGGCATTGTATGGACACATACCGAGATATGGAGCAGCTAAATAAACTTTGGCAGGTAGGGCATGC
TCCCTGGGAGGTTTGGAAAA

Downstream 100 bases:

>100_bases
CGTGGTTTTGTCGGGGGCATAAGGGGTTTTCCAAGGACGTGAATGAAATTGGGAGTAAAAAAGAGAGTTCTAGTGACGGG
GGCCAGTGGTTTTATAGGAA

Product: NAD-dependent epimerase/dehydratase

Products: CDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNLWQNKNVLITGCSGIIGSWLTRRLVDEGANVVGIVRDRLGYGNLLQEQTINRMVVAHGDITDFLFMSRVMAEYEIDTV
FHLAAQTIVTIANRSPLSTFESNLRGTWIVLEACRLSPTVERVVVASSDKAYGDSKELPYREDQPLRGKHPYDVSKSCTD
LLAQSYYHTYRLPVAITRLANVYGGGDLNFNRIIPGTIKAVLEGRPPVLRSDGSPLREYLYVEDAVDAYLTLAKNLHRSN
VMGEAFNFAPQQPYQVLEIVQEIIRIAGKEFVPEIRKHMNGEILHQYSDSSKAKERLNWHTRWDLTKGLSVTIEWYRKFL
MDRRPVKYTME

Sequences:

>Translated_331_residues
MNLWQNKNVLITGCSGIIGSWLTRRLVDEGANVVGIVRDRLGYGNLLQEQTINRMVVAHGDITDFLFMSRVMAEYEIDTV
FHLAAQTIVTIANRSPLSTFESNLRGTWIVLEACRLSPTVERVVVASSDKAYGDSKELPYREDQPLRGKHPYDVSKSCTD
LLAQSYYHTYRLPVAITRLANVYGGGDLNFNRIIPGTIKAVLEGRPPVLRSDGSPLREYLYVEDAVDAYLTLAKNLHRSN
VMGEAFNFAPQQPYQVLEIVQEIIRIAGKEFVPEIRKHMNGEILHQYSDSSKAKERLNWHTRWDLTKGLSVTIEWYRKFL
MDRRPVKYTME
>Mature_331_residues
MNLWQNKNVLITGCSGIIGSWLTRRLVDEGANVVGIVRDRLGYGNLLQEQTINRMVVAHGDITDFLFMSRVMAEYEIDTV
FHLAAQTIVTIANRSPLSTFESNLRGTWIVLEACRLSPTVERVVVASSDKAYGDSKELPYREDQPLRGKHPYDVSKSCTD
LLAQSYYHTYRLPVAITRLANVYGGGDLNFNRIIPGTIKAVLEGRPPVLRSDGSPLREYLYVEDAVDAYLTLAKNLHRSN
VMGEAFNFAPQQPYQVLEIVQEIIRIAGKEFVPEIRKHMNGEILHQYSDSSKAKERLNWHTRWDLTKGLSVTIEWYRKFL
MDRRPVKYTME

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=28.486646884273, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=25.7575757575758, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI56237023, Length=342, Percent_Identity=25.1461988304094, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI56118217, Length=342, Percent_Identity=25.1461988304094, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI189083684, Length=342, Percent_Identity=25.1461988304094, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4504031, Length=328, Percent_Identity=23.1707317073171, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=29.585798816568, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1788353, Length=339, Percent_Identity=28.9085545722714, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1786974, Length=332, Percent_Identity=27.4096385542169, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=29.2682926829268, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982035, Length=343, Percent_Identity=23.9067055393586, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=345, Percent_Identity=24.0579710144928, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=180, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=332, Percent_Identity=26.2048192771084, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=178, Percent_Identity=31.4606741573034, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=332, Percent_Identity=26.2048192771084, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=178, Percent_Identity=31.4606741573034, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=332, Percent_Identity=26.2048192771084, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=23.6526946107784, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=238, Percent_Identity=28.1512605042017, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=341, Percent_Identity=24.0469208211144, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.969696969697, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19923002, Length=336, Percent_Identity=27.6785714285714, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.45

Molecular weight: Translated: 37814; Mature: 37814

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLWQNKNVLITGCSGIIGSWLTRRLVDEGANVVGIVRDRLGYGNLLQEQTINRMVVAHG
CCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHHHHHEEEECC
DITDFLFMSRVMAEYEIDTVFHLAAQTIVTIANRSPLSTFESNLRGTWIVLEACRLSPTV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEEHHCCCCH
ERVVVASSDKAYGDSKELPYREDQPLRGKHPYDVSKSCTDLLAQSYYHTYRLPVAITRLA
HHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NVYGGGDLNFNRIIPGTIKAVLEGRPPVLRSDGSPLREYLYVEDAVDAYLTLAKNLHRSN
HHHCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VMGEAFNFAPQQPYQVLEIVQEIIRIAGKEFVPEIRKHMNGEILHQYSDSSKAKERLNWH
CCHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEHHHCCHHHHHHHCCCC
TRWDLTKGLSVTIEWYRKFLMDRRPVKYTME
HHCCCCCCCHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MNLWQNKNVLITGCSGIIGSWLTRRLVDEGANVVGIVRDRLGYGNLLQEQTINRMVVAHG
CCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHHHHHEEEECC
DITDFLFMSRVMAEYEIDTVFHLAAQTIVTIANRSPLSTFESNLRGTWIVLEACRLSPTV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEEHHCCCCH
ERVVVASSDKAYGDSKELPYREDQPLRGKHPYDVSKSCTDLLAQSYYHTYRLPVAITRLA
HHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NVYGGGDLNFNRIIPGTIKAVLEGRPPVLRSDGSPLREYLYVEDAVDAYLTLAKNLHRSN
HHHCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VMGEAFNFAPQQPYQVLEIVQEIIRIAGKEFVPEIRKHMNGEILHQYSDSSKAKERLNWH
CCHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEHHHCCHHHHHHHCCCC
TRWDLTKGLSVTIEWYRKFLMDRRPVKYTME
HHCCCCCCCHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CDPglucose

Specific reaction: CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]