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The map label for this gene is rfbB [C]

Identifier: 134299745

GI number: 134299745

Start: 2083682

End: 2084617

Strand: Reverse

Name: rfbB [C]

Synonym: Dred_1895

Alternate gene names: 134299745

Gene position: 2084617-2083682 (Counterclockwise)

Preceding gene: 134299746

Following gene: 134299744

Centisome position: 57.78

GC content: 46.79

Gene sequence:

>936_bases
ATGAAATTGGGAGTAAAAAAGAGAGTTCTAGTGACGGGGGCCAGTGGTTTTATAGGAACCCATCTGGTTAGTCGTTTATG
TAAGGAGAATATGCAGGTAGGTGTACTTCGCAAGAGTAAACTCGTGGGAACTTTAAAAAATGACCATGAAGAATTCTTAC
AGGCAAATATTCTTGATTATGACCAGATGAAAGCTGTGGTTCAATACTTTCAACCAGAAATTGTGTGCCACCTAGCGGGG
TTAAGACCTAATGGACATTCCTGGAGAGATGTGTTACAGGCCTATGAAATCAACTTATTGGGGACCATGAACCTTCTACG
TTCCTTGCAGGGTGTAAATTGCCAGTCTGTTATATTGGTAGGGAGTGTTGCCGAATACGGGAGGGGGCCTACACCGTACC
GGGAACACCAGGCATTGTACCCGACCTCTGCCTACGGCACAGCAAAGGCTGCTGCCACGGCCTTAGGTTGTCTTTGCTAT
AACTATTTCAAGTTGCCGGTGGTTACTTTAAGGCTGGCCCTGGTTTACGGACCAGGGCAGGGAGAACAATTTTTTTTGAG
TCAGTTAATAAAGAGTTTATTATTGGAGCAGCCCTTTGCCATGACTGGCGGCGAGCAGTATAGGGATTTTATTCATGTAA
ACGACGTGGTAGAGGCATTATGGCTGGCAGCCAATACCCCAAGGGCAAGGGGTGGGATATTGAATATTGGCATGGGTCAA
TCCTATCCGCTGAAGGAGGTGGCAATGACCGTTGCCACCTCGCTAGGACGCACCGAGCTTTTACGAATTGGTGAAAGGCC
CTATGCACAGGGGGAACAGTTTGCCTATTGTGTGGATACACAACTGGCACGACAGGTTCTAAACTGGCAACCTAAGGTAT
CATTGGAAAAGGGGATTATGGATACCATCAGTTGGTACCAAGGGACTTTGCTATAG

Upstream 100 bases:

>100_bases
CGAATGGTACCGCAAGTTTCTGATGGATCGAAGACCAGTCAAGTATACCATGGAGTGACGTGGTTTTGTCGGGGGCATAA
GGGGTTTTCCAAGGACGTGA

Downstream 100 bases:

>100_bases
AAAAAACTTGGCTTACGCCATACCCTATAGGGCACGAGAGCCTTTAGGCGACGGTGTAAGCCTTAGTTGCCCTTATGCAA
GTCGGAAAATTTTATACTTT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKLGVKKRVLVTGASGFIGTHLVSRLCKENMQVGVLRKSKLVGTLKNDHEEFLQANILDYDQMKAVVQYFQPEIVCHLAG
LRPNGHSWRDVLQAYEINLLGTMNLLRSLQGVNCQSVILVGSVAEYGRGPTPYREHQALYPTSAYGTAKAAATALGCLCY
NYFKLPVVTLRLALVYGPGQGEQFFLSQLIKSLLLEQPFAMTGGEQYRDFIHVNDVVEALWLAANTPRARGGILNIGMGQ
SYPLKEVAMTVATSLGRTELLRIGERPYAQGEQFAYCVDTQLARQVLNWQPKVSLEKGIMDTISWYQGTLL

Sequences:

>Translated_311_residues
MKLGVKKRVLVTGASGFIGTHLVSRLCKENMQVGVLRKSKLVGTLKNDHEEFLQANILDYDQMKAVVQYFQPEIVCHLAG
LRPNGHSWRDVLQAYEINLLGTMNLLRSLQGVNCQSVILVGSVAEYGRGPTPYREHQALYPTSAYGTAKAAATALGCLCY
NYFKLPVVTLRLALVYGPGQGEQFFLSQLIKSLLLEQPFAMTGGEQYRDFIHVNDVVEALWLAANTPRARGGILNIGMGQ
SYPLKEVAMTVATSLGRTELLRIGERPYAQGEQFAYCVDTQLARQVLNWQPKVSLEKGIMDTISWYQGTLL
>Mature_311_residues
MKLGVKKRVLVTGASGFIGTHLVSRLCKENMQVGVLRKSKLVGTLKNDHEEFLQANILDYDQMKAVVQYFQPEIVCHLAG
LRPNGHSWRDVLQAYEINLLGTMNLLRSLQGVNCQSVILVGSVAEYGRGPTPYREHQALYPTSAYGTAKAAATALGCLCY
NYFKLPVVTLRLALVYGPGQGEQFFLSQLIKSLLLEQPFAMTGGEQYRDFIHVNDVVEALWLAANTPRARGGILNIGMGQ
SYPLKEVAMTVATSLGRTELLRIGERPYAQGEQFAYCVDTQLARQVLNWQPKVSLEKGIMDTISWYQGTLL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=27.6073619631902, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI42516563, Length=324, Percent_Identity=24.6913580246914, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788353, Length=338, Percent_Identity=25.7396449704142, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786974, Length=333, Percent_Identity=25.5255255255255, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=326, Percent_Identity=26.3803680981595, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=340, Percent_Identity=24.1176470588235, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=320, Percent_Identity=25.3125, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=342, Percent_Identity=24.2690058479532, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=325, Percent_Identity=22.1538461538462, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=339, Percent_Identity=24.7787610619469, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=25.2307692307692, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=26.3313609467456, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 34519; Mature: 34519

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGVKKRVLVTGASGFIGTHLVSRLCKENMQVGVLRKSKLVGTLKNDHEEFLQANILDY
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCH
DQMKAVVQYFQPEIVCHLAGLRPNGHSWRDVLQAYEINLLGTMNLLRSLQGVNCQSVILV
HHHHHHHHHHCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GSVAEYGRGPTPYREHQALYPTSAYGTAKAAATALGCLCYNYFKLPVVTLRLALVYGPGQ
ECHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GEQFFLSQLIKSLLLEQPFAMTGGEQYRDFIHVNDVVEALWLAANTPRARGGILNIGMGQ
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
SYPLKEVAMTVATSLGRTELLRIGERPYAQGEQFAYCVDTQLARQVLNWQPKVSLEKGIM
CCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHH
DTISWYQGTLL
HHHHHHHCCCC
>Mature Secondary Structure
MKLGVKKRVLVTGASGFIGTHLVSRLCKENMQVGVLRKSKLVGTLKNDHEEFLQANILDY
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCH
DQMKAVVQYFQPEIVCHLAGLRPNGHSWRDVLQAYEINLLGTMNLLRSLQGVNCQSVILV
HHHHHHHHHHCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GSVAEYGRGPTPYREHQALYPTSAYGTAKAAATALGCLCYNYFKLPVVTLRLALVYGPGQ
ECHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
GEQFFLSQLIKSLLLEQPFAMTGGEQYRDFIHVNDVVEALWLAANTPRARGGILNIGMGQ
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
SYPLKEVAMTVATSLGRTELLRIGERPYAQGEQFAYCVDTQLARQVLNWQPKVSLEKGIM
CCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHHH
DTISWYQGTLL
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]