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The map label for this gene is miaA

Identifier: 134299733

GI number: 134299733

Start: 2066041

End: 2066892

Strand: Reverse

Name: miaA

Synonym: Dred_1882

Alternate gene names: 134299733

Gene position: 2066892-2066041 (Counterclockwise)

Preceding gene: 134299734

Following gene: 134299732

Centisome position: 57.28

GC content: 42.61

Gene sequence:

>852_bases
GTGATTTCTGCTGACTCCATGTTAGTTTATCGTGACATGGACATTGGAACGGCTAAACCCACCCTTGAAGAGATGGCAGG
CATTCCCCACCATATGATTGACATTGTTAACCCTAATGAAGAATTCAGTGTAGCCACGTATCAAAGTAGAGTGGAGGAAC
TCATTAACCAAATTATTGATCGTGGCAATTTGCCTCTCCTGGTGGGTGGTACGGGGCTTTATATTCGATCTGTAATTGAT
CACTATGATTTTACCACAGCACCGAAGGATGATCGGTTGAGAGAGTGTCTTAAGCGTGAAGCAGAACAAGTGGGGGCGGC
AGCCATGCATAAAAAATTATCTGAGGTTGATCCACAATCAGCTGAACGCCTGCACCCCAATGACCTGAGACGGGTCATCC
GGGCTTTGGAGGTTTATTATCAAACCGGGAAAACAATTGCGGAATACCAGTATAAAGATCAAGTAGAGAAGCCGAAATAT
AACTTAAAAATGTTTGGGTTAACGATGGATCGGCAATTACTATACCAAAGGATTGAACAGCGGGTCGATCTGATGATGGC
AAGAGGGTTGCTGACTGAGGTAAAGGAATTGGTGGAGCAATACAATGGCCTGGGAACTGCATTACAAGGGCTGGGTTATA
AAGAAATTATCGGCTACTTAAAGGGAGAGTATTCTTTGCCGGAGGCCGTAGAGATTCTTAAACGAAATACTAGACGTTTT
GCCAAACGCCAACTTACGTGGTTTCGAGCTGATAATAGAATTTTTTGGATTGAAATGGATCGTTTTGAAAATAAAAAGGC
TGTTGCGAATGAAATTATGAAGCAAATGGCAGGAGATTTTTTAACTTTGTAG

Upstream 100 bases:

>100_bases
TAAAATAGAAGCATTAAAGCCGTTAATAGCCATTGTAGGGCCTACGGCTTCTGGCAAAACTAATGTAGCTATAGAACTAG
CTAAAAGAGTAAAGGGGGAA

Downstream 100 bases:

>100_bases
AAACATTTAAATAAAAATAGAAAATATTTTCCAATGGAGGGAACCCTCATGACTAAACCCCAGATAAATTTACAAGATGC
CTTTCTGAATCAAGTTAGAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MISADSMLVYRDMDIGTAKPTLEEMAGIPHHMIDIVNPNEEFSVATYQSRVEELINQIIDRGNLPLLVGGTGLYIRSVID
HYDFTTAPKDDRLRECLKREAEQVGAAAMHKKLSEVDPQSAERLHPNDLRRVIRALEVYYQTGKTIAEYQYKDQVEKPKY
NLKMFGLTMDRQLLYQRIEQRVDLMMARGLLTEVKELVEQYNGLGTALQGLGYKEIIGYLKGEYSLPEAVEILKRNTRRF
AKRQLTWFRADNRIFWIEMDRFENKKAVANEIMKQMAGDFLTL

Sequences:

>Translated_283_residues
MISADSMLVYRDMDIGTAKPTLEEMAGIPHHMIDIVNPNEEFSVATYQSRVEELINQIIDRGNLPLLVGGTGLYIRSVID
HYDFTTAPKDDRLRECLKREAEQVGAAAMHKKLSEVDPQSAERLHPNDLRRVIRALEVYYQTGKTIAEYQYKDQVEKPKY
NLKMFGLTMDRQLLYQRIEQRVDLMMARGLLTEVKELVEQYNGLGTALQGLGYKEIIGYLKGEYSLPEAVEILKRNTRRF
AKRQLTWFRADNRIFWIEMDRFENKKAVANEIMKQMAGDFLTL
>Mature_283_residues
MISADSMLVYRDMDIGTAKPTLEEMAGIPHHMIDIVNPNEEFSVATYQSRVEELINQIIDRGNLPLLVGGTGLYIRSVID
HYDFTTAPKDDRLRECLKREAEQVGAAAMHKKLSEVDPQSAERLHPNDLRRVIRALEVYYQTGKTIAEYQYKDQVEKPKY
NLKMFGLTMDRQLLYQRIEQRVDLMMARGLLTEVKELVEQYNGLGTALQGLGYKEIIGYLKGEYSLPEAVEILKRNTRRF
AKRQLTWFRADNRIFWIEMDRFENKKAVANEIMKQMAGDFLTL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=280, Percent_Identity=32.1428571428571, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1790613, Length=283, Percent_Identity=36.3957597173145, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=277, Percent_Identity=28.8808664259928, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=265, Percent_Identity=33.9622641509434, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24649830, Length=315, Percent_Identity=30.4761904761905, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_DESRM (A4J5Q1)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113229.1
- ProteinModelPortal:   A4J5Q1
- SMR:   A4J5Q1
- STRING:   A4J5Q1
- GeneID:   4958205
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1882
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A4J5Q1
- BioCyc:   DRED349161:DRED_1882-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 32832; Mature: 32832

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISADSMLVYRDMDIGTAKPTLEEMAGIPHHMIDIVNPNEEFSVATYQSRVEELINQIID
CCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHC
RGNLPLLVGGTGLYIRSVIDHYDFTTAPKDDRLRECLKREAEQVGAAAMHKKLSEVDPQS
CCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AERLHPNDLRRVIRALEVYYQTGKTIAEYQYKDQVEKPKYNLKMFGLTMDRQLLYQRIEQ
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHH
RVDLMMARGLLTEVKELVEQYNGLGTALQGLGYKEIIGYLKGEYSLPEAVEILKRNTRRF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKRQLTWFRADNRIFWIEMDRFENKKAVANEIMKQMAGDFLTL
HHHHHHHEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISADSMLVYRDMDIGTAKPTLEEMAGIPHHMIDIVNPNEEFSVATYQSRVEELINQIID
CCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHC
RGNLPLLVGGTGLYIRSVIDHYDFTTAPKDDRLRECLKREAEQVGAAAMHKKLSEVDPQS
CCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AERLHPNDLRRVIRALEVYYQTGKTIAEYQYKDQVEKPKYNLKMFGLTMDRQLLYQRIEQ
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHH
RVDLMMARGLLTEVKELVEQYNGLGTALQGLGYKEIIGYLKGEYSLPEAVEILKRNTRRF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKRQLTWFRADNRIFWIEMDRFENKKAVANEIMKQMAGDFLTL
HHHHHHHEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA