BacMap

Definition	Desulfotomaculum reducens MI-1 chromosome, complete genome.
Accession	NC_009253
Length	3,608,104

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The map label for this gene is crt [H]

Identifier: 134299351

GI number: 134299351

Start: 1615228

End: 1616007

Strand: Reverse

Name: crt [H]

Synonym: Dred_1492

Alternate gene names: 134299351

Gene position: 1616007-1615228 (Counterclockwise)

Preceding gene: 134299352

Following gene: 134299350

Centisome position: 44.79

GC content: 44.74

Gene sequence:

>780_bases
ATGGATTACAAAAACTTACTATACAGCAAGGAAGAGAACATTGCCATCATTACTTTGAACAGACCCAAGGTGCTTAATGC
TCTAAACCAGGAGATTATCTCCGAGCTGCACAATCTTTTTGATCAGCTGGCTGGGGACGAGGAAGTAAAGGTTGTCATTC
TTACCGGTGGCGAAAAGGTTTTTGCTGCTGGGGCCGATATTCCTTTCATGCTGGGACTAACTCCCAAACAGGCCGAAGAA
TTAGCAACTTCATTCCACGCAGCCTTTGACCGTATTGAATCTTTGAATAAACCCGTCATTGCGGCCATAGCTGGTTTTGC
CCTGGGTGGTGGCTGCGAGTTGTCCATGGCCTGCGATTTGAGAATTGCCACTGAAGATGCTAAGTTTGGACAACCCGAGA
TCAATCTGGGCGTTATTCCCGGTGCCGGAGGAACCCAGCGACTGTCTCGGTTAGTTGGAGTATCTCGGTCAAAGGAACTA
ATTTATACCGGAAAGATCATTGATGCTCCCACTGCGCTTTCGTACGGTTTAATTAACGAAATTGTTAAGGCCGAAGATTT
ACTAGCCAAGGCAAAAAAATTAGCCAAAGGTTTAGCCTCAAAACCCCCTGTGGCCCTAGGTGCTGCCAAAAGAGCCATTA
ATTATGGTGTAGAGGTTGATAAGAATACCGGCAATTGCTTTGAGAGACAGTGTTTTGCCTTACTATTCTCCACCCAAGAT
CAAAAAGAGGGTATGAATGCCTTTTTAGAAAAACGTAAAGCGGAATTTAAAGGAAATTAA

Upstream 100 bases:

>100_bases
GTCCAGCCTTGTTGTTAAAGCAGATGGTTAGAGCCGGGCATTTGGGTGTTAAAACCGGAAGGGGCTTCTACAGTTACCAG
AAATAGATGGGGGTGAAACA

Downstream 100 bases:

>100_bases
CACTTACGAGGAGGTAAAAGGTTTGAGAAATGTTGTTATTGTTGGGGCCGCCAGGACACCCATAGGAGATTACCTGGGTT
CCCTCAAAGATATTTCAGCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDYKNLLYSKEENIAIITLNRPKVLNALNQEIISELHNLFDQLAGDEEVKVVILTGGEKVFAAGADIPFMLGLTPKQAEE
LATSFHAAFDRIESLNKPVIAAIAGFALGGGCELSMACDLRIATEDAKFGQPEINLGVIPGAGGTQRLSRLVGVSRSKEL
IYTGKIIDAPTALSYGLINEIVKAEDLLAKAKKLAKGLASKPPVALGAAKRAINYGVEVDKNTGNCFERQCFALLFSTQD
QKEGMNAFLEKRKAEFKGN

Sequences:

>Translated_259_residues
MDYKNLLYSKEENIAIITLNRPKVLNALNQEIISELHNLFDQLAGDEEVKVVILTGGEKVFAAGADIPFMLGLTPKQAEE
LATSFHAAFDRIESLNKPVIAAIAGFALGGGCELSMACDLRIATEDAKFGQPEINLGVIPGAGGTQRLSRLVGVSRSKEL
IYTGKIIDAPTALSYGLINEIVKAEDLLAKAKKLAKGLASKPPVALGAAKRAINYGVEVDKNTGNCFERQCFALLFSTQD
QKEGMNAFLEKRKAEFKGN
>Mature_259_residues
MDYKNLLYSKEENIAIITLNRPKVLNALNQEIISELHNLFDQLAGDEEVKVVILTGGEKVFAAGADIPFMLGLTPKQAEE
LATSFHAAFDRIESLNKPVIAAIAGFALGGGCELSMACDLRIATEDAKFGQPEINLGVIPGAGGTQRLSRLVGVSRSKEL
IYTGKIIDAPTALSYGLINEIVKAEDLLAKAKKLAKGLASKPPVALGAAKRAINYGVEVDKNTGNCFERQCFALLFSTQD
QKEGMNAFLEKRKAEFKGN

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=44.3548387096774, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=38.2575757575758, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI150378541, Length=258, Percent_Identity=32.9457364341085, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=35.8208955223881, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI20127408, Length=217, Percent_Identity=33.6405529953917, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI70995211, Length=228, Percent_Identity=32.4561403508772, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI157694516, Length=215, Percent_Identity=29.3023255813954, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI213417737, Length=215, Percent_Identity=29.3023255813954, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=27.6264591439689, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI37594469, Length=241, Percent_Identity=30.7053941908714, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594471, Length=241, Percent_Identity=30.7053941908714, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI157694520, Length=182, Percent_Identity=28.021978021978, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=24.5210727969349, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=24.5210727969349, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI221136756, Length=261, Percent_Identity=24.904214559387, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI221136753, Length=261, Percent_Identity=24.904214559387, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI221307494, Length=261, Percent_Identity=24.904214559387, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=24.1379310344828, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=24.1379310344828, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4757966, Length=237, Percent_Identity=24.4725738396624, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538581, Length=237, Percent_Identity=24.4725738396624, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=41.2371134020619, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=260, Percent_Identity=25.3846153846154, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=216, Percent_Identity=26.3888888888889, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI62530384, Length=245, Percent_Identity=24.0816326530612, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=41.2451361867704, Blast_Score=203, Evalue=9e-54,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=32.5670498084291, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=43.455497382199, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=31.5384615384615, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790281, Length=189, Percent_Identity=37.5661375661376, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=33.976833976834, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=31.6981132075472, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=43.6507936507937, Blast_Score=210, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=41.5322580645161, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=39.0350877192982, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17534483, Length=242, Percent_Identity=30.9917355371901, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=31.1203319502075, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=179, Percent_Identity=38.5474860335196, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17508951, Length=222, Percent_Identity=35.1351351351351, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=222, Percent_Identity=35.1351351351351, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=36.3128491620112, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=28.6821705426357, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25144157, Length=188, Percent_Identity=34.5744680851064, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=188, Percent_Identity=34.5744680851064, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=28.0952380952381, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=213, Percent_Identity=28.169014084507, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=26.6393442622951, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=171, Percent_Identity=23.3918128654971, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=30.3921568627451, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=46.530612244898, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=46.530612244898, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=38.4920634920635, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=31.0810810810811, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21357171, Length=208, Percent_Identity=33.1730769230769, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=25.968992248062, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=29.8578199052133, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=37.5, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=37.5, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=37.5, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571730, Length=152, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571729, Length=152, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24654903, Length=216, Percent_Identity=24.537037037037, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921018, Length=198, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583165, Length=167, Percent_Identity=25.1497005988024, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27901; Mature: 27901

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKNLLYSKEENIAIITLNRPKVLNALNQEIISELHNLFDQLAGDEEVKVVILTGGEKV
CCHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEE
FAAGADIPFMLGLTPKQAEELATSFHAAFDRIESLNKPVIAAIAGFALGGGCELSMACDL
EEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEE
RIATEDAKFGQPEINLGVIPGAGGTQRLSRLVGVSRSKELIYTGKIIDAPTALSYGLINE
EEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEECCEECCCHHHHHHHHHH
IVKAEDLLAKAKKLAKGLASKPPVALGAAKRAINYGVEVDKNTGNCFERQCFALLFSTQD
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCC
QKEGMNAFLEKRKAEFKGN
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDYKNLLYSKEENIAIITLNRPKVLNALNQEIISELHNLFDQLAGDEEVKVVILTGGEKV
CCHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEE
FAAGADIPFMLGLTPKQAEELATSFHAAFDRIESLNKPVIAAIAGFALGGGCELSMACDL
EEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEE
RIATEDAKFGQPEINLGVIPGAGGTQRLSRLVGVSRSKELIYTGKIIDAPTALSYGLINE
EEEECCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEECCEECCCHHHHHHHHHH
IVKAEDLLAKAKKLAKGLASKPPVALGAAKRAINYGVEVDKNTGNCFERQCFALLFSTQD
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCC
QKEGMNAFLEKRKAEFKGN
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]