Click here to switch to the map view.

The map label for this gene is nfo

Identifier: 13357866

GI number: 13357866

Start: 350885

End: 351796

Strand: Direct

Name: nfo

Synonym: UU306

Alternate gene names: 13357866

Gene position: 350885-351796 (Clockwise)

Preceding gene: 13357865

Following gene: 13357869

Centisome position: 46.68

GC content: 26.97

Gene sequence:

>912_bases
ATGGATAAATATAATTTAATTATAGGTAGTCATGTAAGTTTAAAAGCTAAAGATTTCTTTTATGGTAGTGTAAAAGAAGC
ATTATCTTATGGTTCTAACACGTTTATGGTTTATACTGGAGCTCCACAAAATACAAAACGTCAACCGATTAAATCATTTA
AAATTGAAGAAGCACATAATTTGTTAAAAAAACATAATATTAATTTGGATGATTTAATTGTTCACGCTCCATATATTATC
AACCCTTGTTCTTCTAAAAAGAATGTTCGTGAACTTGCTAAAGAATTTTTAATTCAGGAAATCCAACGAACAGAATCTAT
GGGGATCACTAAACTTGTTTTACATCCAGGATCACGTTTAGAACAAAATGAAGATATAGCTTTAGAACAAGTTTATACAA
TGTTAAATGATATTTTTTCAACTATTAATACTAATGTAATTGTTTGCTTAGAGACCATGGCTGGTAAAGGTTCTGAAATT
GGGGTAAATATAAAACAATTAAAAACCATTATAGACAATGTTCATTCTAAAAAAAATATTGGCGTTTGTTTAGATACTTG
CCATATGAATGATAGCGGTTTGATTTTAGATTATTATAATTTTAATCAATATTTAAAAGAATTTGATGCACAAATTGGTA
TTAATTATATTAAAGTATTACATATTAACGATTCTAAAAACCCTTGTGGTGCAAACAAAGACCGCCACGAAAATTTAGGA
TATGGAACGATAGGTTTTGCAAATTTAATTAATATTATTTACCATCCCTTATTAAATAACATCCCTAAAATTTTAGAAAC
GCCTTGGTTTAATGTTAATGATGAATTAATTCCTTTATATAAGCATGAAATAAAAATGATTCGCGACTGCAAGTGGTATG
ACATTAAAAACAAATTATTATCGAAGAAATAA

Upstream 100 bases:

>100_bases
ACTGAAGAAGTTTTAAATTGATATAAACCAATTAAGTTTAAAAAATAATTGTAAGTAAATTAATTAAATTTCTATTTTAA
GGATGATGAAAATGAATAAT

Downstream 100 bases:

>100_bases
AAACTATTTTTAAATACAAATAAGTATTTCTAAAAAAAAAAAAAAAAAGGAGAAGTTCTCCTTTTTTTCGATCACAATTT
TTTATGATCTTATTGGTTAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAPYII
NPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEI
GVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLG
YGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK

Sequences:

>Translated_303_residues
MDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAPYII
NPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEI
GVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLG
YGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK
>Mature_303_residues
MDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAPYII
NPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEI
GVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLG
YGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=262, Percent_Identity=30.5343511450382, Blast_Score=130, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17531193, Length=275, Percent_Identity=32.7272727272727, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322735, Length=293, Percent_Identity=33.4470989761092, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_UREPA (Q9PQI5)

Other databases:

- EMBL:   AF222894
- RefSeq:   NP_078140.1
- ProteinModelPortal:   Q9PQI5
- SMR:   Q9PQI5
- GeneID:   875805
- GenomeReviews:   AF222894_GR
- KEGG:   uur:UU306
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   UURE95667:UU306-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 34705; Mature: 34705

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHN
CCCEEEEEECCEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHHHHH
LLKKHNINLDDLIVHAPYIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRL
HHHHCCCCHHHHEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEIGVNIKQLKTIIDNVHSKKNI
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCC
GVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLG
CEEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCC
YGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHH
SKK
CCC
>Mature Secondary Structure
MDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHN
CCCEEEEEECCEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHHHHH
LLKKHNINLDDLIVHAPYIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRL
HHHHCCCCHHHHEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEIGVNIKQLKTIIDNVHSKKNI
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCC
GVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHENLG
CEEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCC
YGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHH
SKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724