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The map label for this gene is spoT/relA

Identifier: 13357843

GI number: 13357843

Start: 317894

End: 320050

Strand: Direct

Name: spoT/relA

Synonym: UU283

Alternate gene names: 13357843

Gene position: 317894-320050 (Clockwise)

Preceding gene: 13357842

Following gene: 13357844

Centisome position: 42.29

GC content: 25.96

Gene sequence:

>2157_bases
ATGAATGAATTAGTTAAAAAAAAATTAGATGATTTATATGATTTTCTTAAACAAAAAAAATATTCAAGCCAAACCATCGA
ATTAGTCCATAAATCATTTGATTTTGCAAACTATTATCATGGTGATCAAACAAGAAAGTCGGGAGAACCATATATCATTC
ATCCAATTGCAACTGTACGAATTTTAGCTTCATGAGATATGGATGAAAGTACATTAGTAGCAGGTATGTTACACGATGTT
TTAGAAGACACAAATTGTCCAGAAGAAGTTATGGAAAAAACTTTTGGTAAAGAAGTGACGCAACTTGTTTGTTTTGTAAC
AAAAGTATCTCTTTATTCAAAAAATCGACGTAATAAAGTTTCACACACCAATCTTGAAGAAAAGTATTCAATTCAAGTGT
TTATGTCAATGACACAAGACATTCGTGCTATGATTATTAAAATTGCTGATCGTTATCATAATATGCAAACTATTCAATAC
TTACGTTTAGAAAAAGCGAAAAGAATTGCAACAGAAACACTAGAAATTTACGCAAATATTTCTGGAAGATTAGGGATGTA
TCGTGTTAAAACAGATTTATTAGATATGTCTTTTAAAATTTTAGACCCACAAAATTATCAATTAGTAAACGATTCAATTA
ATAAATTGGTTGAAAACAATCAAAAAAAATGAAATGAAATTTTAGCTCGTTTAAAAAATATTTTAATAGCTAATAATATT
AAGGCTGAATTTGAATGGCGTCTTAAAGGTATTTACTCAACATATAAAAAAATAACTAGTGGTTATGAAGTTAAAGATAT
TCATGATATCTATGCTATACGTGTAATTTTAGATGATGTATATTTATGTTATCAAACATTAGGTTTAGTACACATGAATT
TTACGTATATTATTAACACTTTTAAAGATTATATTTCAACACCAAAATGAAATTTATACCAATCTATTCATACAACAATT
TCGTATGAAGGAACTTTAGTAGAAATTCAAATCAGAACAACAAAAATGAATTTATTTGCTAATAATGGTTTAGCAGCACA
CTGAAAATATAAAGAACAAAAAGATAATAAAACTGAAATTTTATTAACAGTTAATAATGTTTTATTGCGTGATTTTTTAT
TGAGCACAGATAAAGGAATTAAAACTATTAAAGATGTCACAACCGGAACTATTTTTGATGTACTAATTCTTAATTCTAAT
AAATGAATCACTGTTTCAAATGGTTCAACATTATTAGATGTAGCATATAAATACGATATAAATAATTTTTTCAATATTTT
AGCAATTTATCGGAATGGTGAGCGTTCACATTTTGACACGCATGTAGAAACTGGCGATACAATTAAAATTAGTTATTCAA
ATGGAATTGAAACAATTAGACCAAACTGGATTACATTAGCTAATAATGATGTTGTTAAATTACATATTAACAATCATTTA
AAAAAATATGAACTGAATAAACAAATTACAAAAGAACAATTTTTAGCTAATGCTGAATTTTTTTTAGAAGGCAAAATTAT
GAGTGAAGAGCATGCTATTAAAAGACTAGAAAAAGAATTTCAAGTTAATAGTATCAATACTTTTTTAGATAATATTGCTA
ATACTGACATTGATTTACAAACAAAATATAAAGTATTTTCAGAAAATAAACAAGTTTCACGTGAAGCAATTAATACAATT
AAAAATCAAGCATGAAAATGATTAGCTGATTCTTCATATTTTAGTGGTCTAGAGAACTTGTATTTTACAGATTTTGAAAT
TTCGCCTTGTTGTTCTAAAATTCCTGGAATTAATTGTGTTGCTAAAATTGTTCGCAATAAAGTAGAAATTCACCGCAATG
ATTGTTCTAAAGTTAAAAATTCTCGCTCAAAAACAATTGTAATTGAATGATCAAAAGAAAAACTTGAGAATCGTCCGCGT
TATTTTAAAGTTAACTTAACATTAAAAGGCAATTGAAGTGAATCTGTTGGAAATGTTTTATGCAACACATTAATACGCAT
GAAAGCAAATATTTCAAAAGTTGATATTAGTAAAAATAAGACTGCACGCACTCATGATACTTTTTTAAAAATTTATGTAA
AAAACTTAGATCACTTACAAAAGATTATTTTAGAACTTCAAGCTAAAAATGTTATTAATGAATGGAGATTGATCTAA

Upstream 100 bases:

>100_bases
ATTAATAATATGAAAACACGAGTTTTAATAACTCGTGTTTTGTTATTATTTAAATAAGTATAATAAATGATTATAACTTA
TTTTTAATAATGGAGTAATT

Downstream 100 bases:

>100_bases
TGAAACATATACAAAAAACAAAATTTATTTTTGTAACTGGTGGTGTTTATTCATCATTAGGTAAAGGGGTTAGTGCTAGT
AGTATTGGTCGAATTCTTGT

Product: ppGpp 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVRILASWDMDESTLVAGMLHDV
LEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVSHTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQY
LRLEKAKRIATETLEIYANISGRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKKWNEILARLKNILIANNI
KAEFEWRLKGIYSTYKKITSGYEVKDIHDIYAIRVILDDVYLCYQTLGLVHMNFTYIINTFKDYISTPKWNLYQSIHTTI
SYEGTLVEIQIRTTKMNLFANNGLAAHWKYKEQKDNKTEILLTVNNVLLRDFLLSTDKGIKTIKDVTTGTIFDVLILNSN
KWITVSNGSTLLDVAYKYDINNFFNILAIYRNGERSHFDTHVETGDTIKISYSNGIETIRPNWITLANNDVVKLHINNHL
KKYELNKQITKEQFLANAEFFLEGKIMSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKVFSENKQVSREAINTI
KNQAWKWLADSSYFSGLENLYFTDFEISPCCSKIPGINCVAKIVRNKVEIHRNDCSKVKNSRSKTIVIEWSKEKLENRPR
YFKVNLTLKGNWSESVGNVLCNTLIRMKANISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI

Sequences:

>Translated_718_residues
MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVRILAS*DMDESTLVAGMLHDV
LEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVSHTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQY
LRLEKAKRIATETLEIYANISGRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKK*NEILARLKNILIANNI
KAEFEWRLKGIYSTYKKITSGYEVKDIHDIYAIRVILDDVYLCYQTLGLVHMNFTYIINTFKDYISTPK*NLYQSIHTTI
SYEGTLVEIQIRTTKMNLFANNGLAAH*KYKEQKDNKTEILLTVNNVLLRDFLLSTDKGIKTIKDVTTGTIFDVLILNSN
K*ITVSNGSTLLDVAYKYDINNFFNILAIYRNGERSHFDTHVETGDTIKISYSNGIETIRPNWITLANNDVVKLHINNHL
KKYELNKQITKEQFLANAEFFLEGKIMSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKVFSENKQVSREAINTI
KNQA*K*LADSSYFSGLENLYFTDFEISPCCSKIPGINCVAKIVRNKVEIHRNDCSKVKNSRSKTIVIE*SKEKLENRPR
YFKVNLTLKGN*SESVGNVLCNTLIRMKANISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI
>Mature_718_residues
MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVRILAS*DMDESTLVAGMLHDV
LEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVSHTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQY
LRLEKAKRIATETLEIYANISGRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKK*NEILARLKNILIANNI
KAEFEWRLKGIYSTYKKITSGYEVKDIHDIYAIRVILDDVYLCYQTLGLVHMNFTYIINTFKDYISTPK*NLYQSIHTTI
SYEGTLVEIQIRTTKMNLFANNGLAAH*KYKEQKDNKTEILLTVNNVLLRDFLLSTDKGIKTIKDVTTGTIFDVLILNSN
K*ITVSNGSTLLDVAYKYDINNFFNILAIYRNGERSHFDTHVETGDTIKISYSNGIETIRPNWITLANNDVVKLHINNHL
KKYELNKQITKEQFLANAEFFLEGKIMSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKVFSENKQVSREAINTI
KNQA*K*LADSSYFSGLENLYFTDFEISPCCSKIPGINCVAKIVRNKVEIHRNDCSKVKNSRSKTIVIE*SKEKLENRPR
YFKVNLTLKGN*SESVGNVLCNTLIRMKANISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=694, Percent_Identity=28.8184438040346, Blast_Score=242, Evalue=7e-65,
Organism=Escherichia coli, GI1789147, Length=600, Percent_Identity=26, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25143118, Length=158, Percent_Identity=34.1772151898734, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811 [H]

Pfam domain/function: PF04607 RelA_SpoT [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 81981; Mature: 81981

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVR
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECHHHHH
ILASDMDESTLVAGMLHDVLEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVS
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQYLRLEKAKRIATETLEIYANIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCC
GRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKKNEILARLKNILIANNIKA
CCCEEEEEHHHHHHCEEEEECCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
EFEWRLKGIYSTYKKITSGYEVKDIHDIYAIRVILDDVYLCYQTLGLVHMNFTYIINTFK
EEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYISTPKNLYQSIHTTISYEGTLVEIQIRTTKMNLFANNGLAAHKYKEQKDNKTEILLTV
HHHCCHHHHHHHHHHEEEECCEEEEEEEEEEEEEEEECCCCCHHHHHHCCCCCEEEEEEE
NNVLLRDFLLSTDKGIKTIKDVTTGTIFDVLILNSNKITVSNGSTLLDVAYKYDINNFFN
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCEEEECCCCEEEEEEEEECHHHHEE
ILAIYRNGERSHFDTHVETGDTIKISYSNGIETIRPNWITLANNDVVKLHINNHLKKYEL
EEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEECCCEEEEECCCEEEEEECCCCHHHHH
NKQITKEQFLANAEFFLEGKIMSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKV
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
FSENKQVSREAINTIKNQAKLADSSYFSGLENLYFTDFEISPCCSKIPGINCVAKIVRNK
HCCCHHHHHHHHHHHHHHHHHCCHHHHHCHHHCEEECCCCCHHHHHCCCHHHHHHHHHHH
VEIHRNDCSKVKNSRSKTIVIESKEKLENRPRYFKVNLTLKGNSESVGNVLCNTLIRMKA
HHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHC
NISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNELVKKKLDDLYDFLKQKKYSSQTIELVHKSFDFANYYHGDQTRKSGEPYIIHPIATVR
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECHHHHH
ILASDMDESTLVAGMLHDVLEDTNCPEEVMEKTFGKEVTQLVCFVTKVSLYSKNRRNKVS
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HTNLEEKYSIQVFMSMTQDIRAMIIKIADRYHNMQTIQYLRLEKAKRIATETLEIYANIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCC
GRLGMYRVKTDLLDMSFKILDPQNYQLVNDSINKLVENNQKKNEILARLKNILIANNIKA
CCCEEEEEHHHHHHCEEEEECCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
EFEWRLKGIYSTYKKITSGYEVKDIHDIYAIRVILDDVYLCYQTLGLVHMNFTYIINTFK
EEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYISTPKNLYQSIHTTISYEGTLVEIQIRTTKMNLFANNGLAAHKYKEQKDNKTEILLTV
HHHCCHHHHHHHHHHEEEECCEEEEEEEEEEEEEEEECCCCCHHHHHHCCCCCEEEEEEE
NNVLLRDFLLSTDKGIKTIKDVTTGTIFDVLILNSNKITVSNGSTLLDVAYKYDINNFFN
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCEEEECCCCEEEEEEEEECHHHHEE
ILAIYRNGERSHFDTHVETGDTIKISYSNGIETIRPNWITLANNDVVKLHINNHLKKYEL
EEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEECCCEEEEECCCEEEEEECCCCHHHHH
NKQITKEQFLANAEFFLEGKIMSEEHAIKRLEKEFQVNSINTFLDNIANTDIDLQTKYKV
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
FSENKQVSREAINTIKNQAKLADSSYFSGLENLYFTDFEISPCCSKIPGINCVAKIVRNK
HCCCHHHHHHHHHHHHHHHHHCCHHHHHCHHHCEEECCCCCHHHHHCCCHHHHHHHHHHH
VEIHRNDCSKVKNSRSKTIVIESKEKLENRPRYFKVNLTLKGNSESVGNVLCNTLIRMKA
HHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHC
NISKVDISKNKTARTHDTFLKIYVKNLDHLQKIILELQAKNVINEWRLI
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]