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The map label for this gene is truA2 [H]

Identifier: 126699393

GI number: 126699393

Start: 2065799

End: 2066536

Strand: Direct

Name: truA2 [H]

Synonym: CD1784

Alternate gene names: 126699393

Gene position: 2065799-2066536 (Clockwise)

Preceding gene: 126699392

Following gene: 126699394

Centisome position: 48.15

GC content: 26.83

Gene sequence:

>738_bases
ATGAGAAACATAAAAATGATAGTAGCATACGATGGTTCAAGATATAAAGGATATCAAAAATTAGGTGACAATAATATGAC
TATTCAAGAAAAACTTGAAAATGTTTTAAGTAAAATGACTAATGAAACAGTGGAGATAATAGGTTCTGGAAGAACTGATA
TGGGAGCTCATGCAAGAGGACAAGTGGTAAATTTTAGAACTAATTGTATGGATTCTTTGGATAAAATTCAAAAATATTTA
TATGAATATTTACCAGAAGATATAGTTGTAAAAACTGTTGAAGAAGTTGATGAAAGATTTCATTCAAGATACAATGTAAA
ATCAAAGACTTATATGTATAAAATTGACAATAATAAGTATCATAATCCTTTTATAAGAAAATATGCTACTCATGTAAGTA
AAAAATTAGATTTAGATAGAATGAGAAAAGCAAGTGAGTATTTAGTAGGAGAACATGATTTTACTAGTTTTGCTTCTTCT
AAATCTAAGAAAAAATCAAATGTAAGAGAAATTTATTCTATAAATATAAAAGAAGATGATAATGTTATAGAAATATATGT
AGAAGGAAATGGATTTTTGTATAACATGGTAAGGATAATAGTAGGTGCTTTGATAGATGTTGGTCTAAAAAGAAGAGCTC
CACAAGATATTAAGTATATGCTTGAATCAAAAGATAGATGTCAATCATCAGATACTGCACCAGCTAAAGGACTATGTCTT
TGGAAAGTAACATATTAA

Upstream 100 bases:

>100_bases
AATGAAAATAGAAAGTGAAAAGAATGTTGGAACAAAAATAACTTTTAATATAAAAAATAAATTTGCTAAACAAGCATAAA
TTCTTAGAAAGAGGATTAAG

Downstream 100 bases:

>100_bases
TCAATTATATAAAACTCTCTTTAAAATAAAAATAAAATAAATTATATAGTTGACATAAATGGAAAAATAAACTATTATAT
ATTTTATAAGAGAGATATAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETVEIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYL
YEYLPEDIVVKTVEEVDERFHSRYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDRCQSSDTAPAKGLCL
WKVTY

Sequences:

>Translated_245_residues
MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETVEIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYL
YEYLPEDIVVKTVEEVDERFHSRYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDRCQSSDTAPAKGLCL
WKVTY
>Mature_245_residues
MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETVEIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYL
YEYLPEDIVVKTVEEVDERFHSRYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDRCQSSDTAPAKGLCL
WKVTY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=253, Percent_Identity=26.4822134387352, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=35.5371900826446, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71985046, Length=186, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28512; Mature: 28512

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETVEIIGSGRTDMGAHARG
CCCEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCC
QVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHSRYNVKSKTYMYKIDNNKY
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
HNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASSKSKKKSNVREIYSINIKEDD
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCC
NVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDRCQSSDTAPAKGLCL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
WKVTY
EEECC
>Mature Secondary Structure
MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETVEIIGSGRTDMGAHARG
CCCEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCC
QVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHSRYNVKSKTYMYKIDNNKY
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
HNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASSKSKKKSNVREIYSINIKEDD
CCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCC
NVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDRCQSSDTAPAKGLCL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
WKVTY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]