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The map label for this gene is vanG [H]

Identifier: 126699230

GI number: 126699230

Start: 1884003

End: 1885103

Strand: Direct

Name: vanG [H]

Synonym: CD1626

Alternate gene names: 126699230

Gene position: 1884003-1885103 (Clockwise)

Preceding gene: 126699229

Following gene: 126699231

Centisome position: 43.91

GC content: 30.15

Gene sequence:

>1101_bases
ATGAATAATAAAAAAATTGCAGTTATATTTGGTGGAAACTCAACAGAATATGATGTATCATTACAATCAGCTTTTGCAGT
ACTTGAGAATTTGAATACGGAAAAATATGATATTCTACCAATTGGAATTACAAGGGAGGGTAATTGGTATCATTATATTG
GAGATTATAATAATATCCAGAATAACACTTGGTTTGAAAGGGATACTGATATAATTCCAGTTATTGTTTCGCAGAACCGT
GTCAAAAATGGTATTATTGAATTTTTAATTTCTGATGCTGTTTCTCAGAATCATATTCCAAATAGTGCTATTGAATCATG
TGAAAATGATATTAAATACACTAAACTTGATTTTGTATTTCCTGTATTGCATGGAAAAAATGGAGAAGATGGAACAGTGC
AGGGGTTATTTGAATTGGCAGGTATACAGGTTATTGGATGTGGTACTCTTTCTTCAGCATTATGTATGGACAAAGACAAA
TCACATAAACTTGTTCATTCAGAAGGTATTAAAGTACCAAAATCAATATTACTAAAATACTCAGATAAATTTAATGTGGC
TGAACTGTCAAAAGACTTAACTTATCCATTATTTATAAAACCTGTGCGTGCAGGTTCATCATTTGGTATTAGTAAAATTT
ATAAAAAAGAAGAGTTACAAGTAGCAGTAGACTTGGCTTTTGAACATGATAATGAAGTAATAATTGAGGAAAATATTGAT
GGTTTTGAGGTAGGGTGTGCAGTTTTAGGAAACAATGACTTACTTGTTGGAAGAGTAGATGAAATAGAATTGTCAAAAGG
ATTTTTTGATTATACAGAAAAATATACATTGAAAAGTTCAAAAATTCATATGCCAGCTAGAATTGATTTGGATTGTGAGA
AAAGAATACAAGAGACAGCAAAAATTATTTATAGAACTCTTGGATGTTCTGGATTTGCTAGAGTAGATATGTTTTTAACT
CCATCTGGTGAAATTATTTTTAATGAAGTCAATACAATTCCTGGCTTTACTTCTCATAGTCGTTATCCAAATATGATGAA
AGGTATTGGACTGTCTTTTGAAGAAATATTGGACAAGATAATTGAGGTGTACATAAAATGA

Upstream 100 bases:

>100_bases
ATTGTAAGTGAAGGTTGATTGGATTTGAAATAGAAAAAGTATTTTAACATGTTTCTGTAGATTGTATAAAAATAAATAAA
TCAACAAAGGAGAATCAAAG

Downstream 100 bases:

>100_bases
TGAATAATATTTTATTAGATAAAAATAAAGAAGATAAAATGACAAATACTATCTTATTGACTAAAGATAAAGTTTATGAT
GGGAATCTAATTCTAGTAAA

Product: vancomycin/teicoplanin A-type resistance protein

Products: NA

Alternate protein names: D-alanine--D-lactate ligase; VanB ligase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGITREGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNR
VKNGIIEFLISDAVSQNHIPNSAIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENID
GFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLT
PSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK

Sequences:

>Translated_366_residues
MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGITREGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNR
VKNGIIEFLISDAVSQNHIPNSAIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENID
GFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLT
PSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK
>Mature_366_residues
MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGITREGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNR
VKNGIIEFLISDAVSQNHIPNSAIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENID
GFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLT
PSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK

Specific function: Required for high-level resistance to glycopeptides antibiotics. D-Ala--D-Ala ligase of altered specificity which catalyzes ester bond formation between D-Ala and various D-hydroxy acids; producing a peptidoglycan which does not terminate in D- alanine bu

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=369, Percent_Identity=35.7723577235772, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1786280, Length=262, Percent_Identity=38.1679389312977, Blast_Score=174, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.1.2.1 [H]

Molecular weight: Translated: 41123; Mature: 41123

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGITREGNWYHYIGDYNNIQ
CCCCEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEECCCCCCC
NNTWFERDTDIIPVIVSQNRVKNGIIEFLISDAVSQNHIPNSAIESCENDIKYTKLDFVF
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEE
PVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDKSHKLVHSEGIKVPKSILLKY
EEEECCCCCCCHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCEEECCCCCCCHHHHEEE
SDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENID
CCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCEEEEEEEEEECCCEEEEECCCC
GFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETA
CEEEEEEEECCCCEEEEECCEEEECCCHHHHCCEEEEECCEEECCEEECCCHHHHHHHHH
KIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKI
HHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHH
IEVYIK
HHHHCC
>Mature Secondary Structure
MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDILPIGITREGNWYHYIGDYNNIQ
CCCCEEEEEECCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEECCCCCCC
NNTWFERDTDIIPVIVSQNRVKNGIIEFLISDAVSQNHIPNSAIESCENDIKYTKLDFVF
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEE
PVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDKSHKLVHSEGIKVPKSILLKY
EEEECCCCCCCHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCCEEECCCCCCCHHHHEEE
SDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENID
CCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCEEEEEEEEEECCCEEEEECCCC
GFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETA
CEEEEEEEECCCCEEEEECCEEEECCCHHHHCCEEEEECCEEECCEEECCCHHHHHHHHH
KIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKI
HHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHH
IEVYIK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8125347; 8631706; 12663927; 8440477; 8215270 [H]