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The map label for this gene is vanS [H]

Identifier: 126699229

GI number: 126699229

Start: 1882600

End: 1883742

Strand: Direct

Name: vanS [H]

Synonym: CD1625

Alternate gene names: 126699229

Gene position: 1882600-1883742 (Clockwise)

Preceding gene: 126699228

Following gene: 126699230

Centisome position: 43.88

GC content: 29.48

Gene sequence:

>1143_bases
TTGAAAAGTAAAAATATTAAAGTCAATCCTGACTTTACTCAAGTGAGAAATAAAATTTTTATGAGAACAGCTTTAATGGC
ATTTATAGCCATTATTTTCATATGGATTTTATATTCTTTTATATTGTATGGTAAGTTTTCTATCTGGATAGTATCATTCT
TTGAAAATGCTTTTCGTATGGAATATAAAGCTGCATTGAATTTATATCAGCAGATTTTCCGTAATAGCATGGAGCTCTTC
ATGATACTAGCTATGGTGATTGTGTTTTTTATAATATTCCGTGTTTATTTAAATTGGTTTACAAAATATTTTAGTGAAAT
AAATCAAGGTATTGACTCTCTTATCAAAGAAGATGTTGGAGAAGTGGCTTTATCTCCAGAGTTATTGGCAATAGAAAAGA
AAATAAACTCAATTAAACATATACTTGAACAACGTAAATTTGAGACACAAATGGCAGAACAACGTAAGAATGAACTTATT
GTTTATCTTGCACATGATTTAAAAACACCACTTACATCAGTTATAGGGTATCTGACACTACTTCGAGATGAAAGTCAGAT
TTCTGAAGAACTGCGAAAAAAATATTTATCTATTTCGTTGGATAAGGCAGAGCGTCTTGAAGATTTAATTAATGAATTCT
TTGAAATTACAAGGTTTAATTTATCAAATATATCACTTGAATACAGTACAGTAAGCTTAACTCGTATGCTTGAACAACTA
ACATATGAGTTTAAACCTACTTTGATGAGTAAGAATTTAAAATATACACTGAATGTTACACCAAATATGATGATAAGGTG
TGATGTTAATAAAATGCAACGTGTATTTGATAACTTATTACGAAATGCTGTTTATTATAGTTTTGAAGATACTACTATTG
AAATAACAGCTGTACAGGAGGATGATTATATAAAGCTTAAATTTATAAATCATAGTAATATGATTCCAGAGGAAAAACTT
GAGCGTATTTTTGAACAATTTTACAGGTTGGATACATCTCGTAATTCAAACAGTGGTAGAGCTGGGTTGGGACTTGCTAT
AGCTAAAGAAATAGTGGAGCTTCATAACGGGACAATAACAGCTCATAGCGAGAACAATATAATTACCTTTAAGGTTATTA
TTCCGATTGTAGGAAATTTGTAA

Upstream 100 bases:

>100_bases
AACACAGTTATGGCTCATATAGGGCGATTACGTGAAAAAATTCATGAACCTTCTAAAAACCCGAAATTTATAAAAACTGT
ATGGGGAGTAGGTTATACAA

Downstream 100 bases:

>100_bases
GAATTTAAGTATAAAAAAATAGGAAATTCTTTAGGATAAATAAATAAAGAGCACTCTTGTTATTGATATAATTACTGTAT
CAAAACAAGAGTGCTTTTAT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELF
MILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLEQL
TYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINHSNMIPEEKL
ERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL

Sequences:

>Translated_380_residues
MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELF
MILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLEQL
TYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINHSNMIPEEKL
ERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL
>Mature_380_residues
MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELF
MILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLEQL
TYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEITAVQEDDYIKLKFINHSNMIPEEKL
ERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTITAHSENNIITFKVIIPIVGNL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=254, Percent_Identity=27.5590551181102, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=26.431718061674, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=28.3950617283951, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=25.8196721311475, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=24.3801652892562, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=29.6803652968037, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788279, Length=265, Percent_Identity=29.0566037735849, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=22.972972972973, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790861, Length=290, Percent_Identity=24.4827586206897, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787894, Length=274, Percent_Identity=29.1970802919708, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789149, Length=258, Percent_Identity=26.7441860465116, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=23.5042735042735, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082128, Length=237, Percent_Identity=26.5822784810127, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790551, Length=350, Percent_Identity=22, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787374, Length=199, Percent_Identity=27.6381909547739, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=24.5535714285714, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=27.4590163934426, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44722; Mature: 44722

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRM
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHHHHH
LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLEQL
HCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
TYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEITAVQE
HHHHCHHHHCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEC
DDYIKLKFINHSNMIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTIT
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEE
AHSENNIITFKVIIPIVGNL
EECCCCEEEEEEEEEHHCCC
>Mature Secondary Structure
MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRM
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHHHHH
LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLEQL
HCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
TYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEITAVQE
HHHHCHHHHCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEC
DDYIKLKFINHSNMIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVELHNGTIT
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEEE
AHSENNIITFKVIIPIVGNL
EECCCCEEEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]