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The map label for this gene is purB [H]

Identifier: 126698933

GI number: 126698933

Start: 1547874

End: 1549319

Strand: Direct

Name: purB [H]

Synonym: CD1334

Alternate gene names: 126698933

Gene position: 1547874-1549319 (Clockwise)

Preceding gene: 126698932

Following gene: 126698934

Centisome position: 36.08

GC content: 31.67

Gene sequence:

>1446_bases
ATGATAGATAATAAATATACTACTTACTCAAACCCACTTACAGACAGATATTGTAGCAAGGACATGAGTTATATATTTTC
TCCACAATTTAAATTCTCTACATGGAGAAGGTTATGGGTTGCACTTGCAGAAGGTGAACATGAGTTAGGAATCAATATAA
CTGAAGAACAATTAGATGAGCTAAGAGCTAATATAGATAATATAAATTTTGATGAAGCTAGAAAAATAGAAAAAGAAGTA
AGACATGATGTAATGTCTCACGTAAAAGCTTATGGTTTACAATGTGAAAAAGCCAAAGGAATAATACATTTAGGAGCAAC
ATCTGCATATGTTGGAGATAATACAGATGTTATACAAATGAATGAAGCTTTAAAACTTCTAAGAGTAAAGCTTGTAAATT
TAATAAACAATTTAAAAGAATTTGCACTTAAGTACAAAGATATGCCAGCTCTTGGATTTACACACTTCCAAGCTGCTCAA
CTTACAACAGTAGGAAAAAGAGCTTGTTTATGGGCTCAAGATTTAATTCTTGATTTGGAAGATTTAAACTATAGAATAGA
CAACATGAAGTTAAGAGGAGTTAAAGGTACTACTGGTACTCAAGCAAGTTTTTTAAGCTTATTTGAAGGAGACCACGAAA
AAGTAAAAGAGTTAGATAAGAAAATTTGTAAAAAGATGGGATACCATTCTTCCTATGCAGTAAGTGGACAGACATATACT
AGAAAGTTAGATTATCAAGTAATAAGCATACTATCAGGGATAGCTCAAAGTATGCATAAGATGACTAATGATATAAGACT
TTTACAAAGTTTAAAAGAATTAGAAGAGCCATTTGAAAAAAATCAAATAGGTTCATCAGCAATGGCTTATAAGAGGAATC
CAATGAGAAGTGAGAGAATAGCATCTTTATCTAAGTATATAATCTCTGAATCTATAAGCCCTGCAATGGTACAAGCCACT
CAATGGTTAGAGAGAACATTAGATGACTCAGCCAATAAGAGATTGGCAATACCTCAAGCATTTATGGCAGCAGATGCTAT
ACTTGAAATAGGGATAAATGTAACTGATGGTTTAGTAGTATATGAAAATATGATAAATAGACGTGTAAATGAGGAACTTC
CTTTTATGGCTACAGAAACAATACTTATGGAAGCTGTAAAAAGAGGTGGAGATAGACAGGAATTACATGAAATAATAAGA
GAATATTCTATGAAAGCTGCCTATAGAGTAAAGCATGAAGGCAAGGATAATAACTTAATTGAACTTATAATGAATGATGA
TTCATTTAAAATGAGCAAGGAAGAAATACTATCTATAATGGACCCAAAAAACTTTATAGGTAGAGCTCCAGAACAAGTTG
TAGAATTTGTAAATGAAGTTGTAGAACCTGCTATAAAAGATTATAAGGAAGACTTAGGAAAAGTAGATGTAGACCTAAGA
GTATAG

Upstream 100 bases:

>100_bases
AAAAGTCTTGTTATAGTTCGTAAAATTATATATAATTATTTATGTACTAATGTGTACACAATAATCACACGACAAAAGAT
TTAAGGAAGGTGCTAATATA

Downstream 100 bases:

>100_bases
AATTGTACAGATAAAATATAATTAAAAACGGCTATCTTATTTATAAATAAGATAGCCATTTTAATGGGCTAGCGTCTTCT
CTCCACCGCTGTGAAGAGTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAEGEHELGINITEEQLDELRANIDNINFDEARKIEKEV
RHDVMSHVKAYGLQCEKAKGIIHLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKELDKKICKKMGYHSSYAVSGQTYT
RKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEEPFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQAT
QWLERTLDDSANKRLAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDRQELHEIIR
EYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAPEQVVEFVNEVVEPAIKDYKEDLGKVDVDLR
V

Sequences:

>Translated_481_residues
MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAEGEHELGINITEEQLDELRANIDNINFDEARKIEKEV
RHDVMSHVKAYGLQCEKAKGIIHLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKELDKKICKKMGYHSSYAVSGQTYT
RKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEEPFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQAT
QWLERTLDDSANKRLAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDRQELHEIIR
EYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAPEQVVEFVNEVVEPAIKDYKEDLGKVDVDLR
V
>Mature_481_residues
MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAEGEHELGINITEEQLDELRANIDNINFDEARKIEKEV
RHDVMSHVKAYGLQCEKAKGIIHLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKELDKKICKKMGYHSSYAVSGQTYT
RKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEEPFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQAT
QWLERTLDDSANKRLAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDRQELHEIIR
EYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAPEQVVEFVNEVVEPAIKDYKEDLGKVDVDLR
V

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=472, Percent_Identity=53.6016949152542, Blast_Score=538, Evalue=1e-153,
Organism=Homo sapiens, GI183227688, Length=413, Percent_Identity=53.5108958837772, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17508577, Length=475, Percent_Identity=39.1578947368421, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32564234, Length=342, Percent_Identity=44.1520467836257, Blast_Score=310, Evalue=8e-85,
Organism=Saccharomyces cerevisiae, GI6323391, Length=480, Percent_Identity=50.625, Blast_Score=498, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24647570, Length=479, Percent_Identity=53.6534446764092, Blast_Score=521, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 55039; Mature: 55039

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAEGEHELGINITEEQLDE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGIIHLGATSAYVGDNTDVIQM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEECCCCCCEEEH
NEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQLTTVGKRACLWAQDLILDLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKELDKKICKKMGYHSSYAVSGQTYT
HCCEEECCEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHH
RKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEEPFEKNQIGSSAMAYKRNPMRSERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHH
ASLSKYIISESISPAMVQATQWLERTLDDSANKRLAIPQAFMAADAILEIGINVTDGLVV
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHH
YENMINRRVNEELPFMATETILMEAVKRGGDRQELHEIIREYSMKAAYRVKHEGKDNNLI
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ELIMNDDSFKMSKEEILSIMDPKNFIGRAPEQVVEFVNEVVEPAIKDYKEDLGKVDVDLR
EEEECCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
V
C
>Mature Secondary Structure
MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAEGEHELGINITEEQLDE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGIIHLGATSAYVGDNTDVIQM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEECCCCCCEEEH
NEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQLTTVGKRACLWAQDLILDLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKELDKKICKKMGYHSSYAVSGQTYT
HCCEEECCEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHH
RKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEEPFEKNQIGSSAMAYKRNPMRSERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHH
ASLSKYIISESISPAMVQATQWLERTLDDSANKRLAIPQAFMAADAILEIGINVTDGLVV
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHH
YENMINRRVNEELPFMATETILMEAVKRGGDRQELHEIIREYSMKAAYRVKHEGKDNNLI
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ELIMNDDSFKMSKEEILSIMDPKNFIGRAPEQVVEFVNEVVEPAIKDYKEDLGKVDVDLR
EEEECCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]