Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 126698888

GI number: 126698888

Start: 1498827

End: 1499990

Strand: Direct

Name: dacF [H]

Synonym: CD1291

Alternate gene names: 126698888

Gene position: 1498827-1499990 (Clockwise)

Preceding gene: 126698887

Following gene: 126698891

Centisome position: 34.94

GC content: 31.96

Gene sequence:

>1164_bases
ATGAAAAAATTAGCTAGTACTGCTTTGGCTATATTAATTGCGCTTACTCCTTTAAGCTTTTCTTTTGCAAATAATAAGGA
AAATGCTGATGCCAACCAACTAAATATATCTTCAAAATCAGCAATTTTGATGGATGTTGGTAGTGGACAAATTTTATATG
AAAAGGATGCTCATAAAAAGCTTCCTCCTGCTAGTGTAACTAAGGTAATGACTATGCTACTTATAGTAGAAGCTTTGGAT
TCAGGCAAGATTAAGCTTGATGATGAAGTTCAAGTAAGTGAAACTGCATCTAGTATGGGTGGTAGTCAAATATTCCTAGA
GCCAGGAGAAACTCAAAAGGTTGATACATTATTAAAAGGTATAGCTGTTGCTTCTGCAAATGATGCTTGTGTTGCTATGG
CTGAACACTTAGCTGGAAGTGTTGAAGGATTTGTTGATAGAATGAATGCTAAAGCAAAAGAGTTAAATATGAATGATACT
CATTTTGCAAATACAAATGGACTTCCAGTTGCAAACCACTATACATCTGCTCATGATATAGCTTTAATGTCTAGAGAACT
ATTAAAACACGAAATGATAAGTAAATACTTAACTACATGGATGGATAAGGTTGTAGTAGGGAAGAAACAAGTTACAGTAG
GTCTTGCAAATACTAATAAACTTATAAAGCATTATCAAGGAGCAACTGGTGTTAAAACTGGTTTTACTCAAGAAGCAAAA
TACTGTTTATCAGCATCTGCAAAGAGAGGTAATACTCATCTAGTTGCTGCAACTTTAGGAGCAGAGACTTCTCCAGAAAG
ATTTAATGATGCCTCTAGCCTTTTAAACTATGGATTTGCTAATTATGAAAGTGTTAAGTTATGTAGTAAAGGTGATAATA
TAGCAACACTTACATTGGATAAGGCTGATGAAAACAAAGTTAAATTGGTTGCTAAAGAAGATTTAAATGCTCTTATTAAA
AAAGGAAGTTCTAAAGAATTTGAGAAGAAAATTGAAATAGTAAAAAATCCTAAGATGCCAATTAAAAAGGGAACTGTTTT
AGGAAAAATAAAGATATGTAAAGATAAAAAAGTAATAGGCGAAGTGGAATTGATAAATACTAAGGATATAAATAAGGCAA
GCTATCTACAAATGCTTCAAAGAATAATTGATAATATGATATAA

Upstream 100 bases:

>100_bases
AAAAGAAAAAAAATTTAGGTCAAGGTATATCTAGGCATTTAGAATAAAAAAAGTTAAATGTGAGAAAATAAGTTTAAATA
AACTTTAAGAGGTGACATCG

Downstream 100 bases:

>100_bases
ATAATTATCAGATGAAATATAATTATAATACACAAAGATATGATTATAATACACATAAAAAATACCCTTATGTAATTATT
TACCATAGGGGTATTTTTAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILMDVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALD
SGKIKLDDEVQVSETASSMGGSQIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANTNKLIKHYQGATGVKTGFTQEAK
YCLSASAKRGNTHLVAATLGAETSPERFNDASSLLNYGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIK
KGSSKEFEKKIEIVKNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI

Sequences:

>Translated_387_residues
MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILMDVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALD
SGKIKLDDEVQVSETASSMGGSQIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANTNKLIKHYQGATGVKTGFTQEAK
YCLSASAKRGNTHLVAATLGAETSPERFNDASSLLNYGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIK
KGSSKEFEKKIEIVKNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI
>Mature_387_residues
MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILMDVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALD
SGKIKLDDEVQVSETASSMGGSQIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANTNKLIKHYQGATGVKTGFTQEAK
YCLSASAKRGNTHLVAATLGAETSPERFNDASSLLNYGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIK
KGSSKEFEKKIEIVKNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=388, Percent_Identity=32.4742268041237, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1786851, Length=361, Percent_Identity=31.8559556786704, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87082022, Length=382, Percent_Identity=29.8429319371728, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082054, Length=252, Percent_Identity=29.7619047619048, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41993; Mature: 41993

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILMDVGSGQILYEKDAHKK
CCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCCEEEEECCCCCEEEECCCCCC
LPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGSQIFLEPGETQKVDTLLKG
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHH
IAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDTHFANTNGLPVANHYTSAHDI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCCCCCCCCCCCCHHHH
ALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANTNKLIKHYQGATGVKTGFTQEAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHH
YCLSASAKRGNTHLVAATLGAETSPERFNDASSLLNYGFANYESVKLCSKGDNIATLTLD
HHHHCCCCCCCEEEEEEECCCCCCCHHHCHHHHHHHCCCCCCCCEEEECCCCCEEEEEEC
KADENKVKLVAKEDLNALIKKGSSKEFEKKIEIVKNPKMPIKKGTVLGKIKICKDKKVIG
CCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCC
EVELINTKDINKASYLQMLQRIIDNMI
EEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILMDVGSGQILYEKDAHKK
CCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCCEEEEECCCCCEEEECCCCCC
LPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGSQIFLEPGETQKVDTLLKG
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHH
IAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDTHFANTNGLPVANHYTSAHDI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCCCCCCCCCCCCHHHH
ALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANTNKLIKHYQGATGVKTGFTQEAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHH
YCLSASAKRGNTHLVAATLGAETSPERFNDASSLLNYGFANYESVKLCSKGDNIATLTLD
HHHHCCCCCCCEEEEEEECCCCCCCHHHCHHHHHHHCCCCCCCCEEEECCCCCEEEEEEC
KADENKVKLVAKEDLNALIKKGSSKEFEKKIEIVKNPKMPIKKGTVLGKIKICKDKKVIG
CCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCC
EVELINTKDINKASYLQMLQRIIDNMI
EEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]