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The map label for this gene is amyB [H]

Identifier: 126698463

GI number: 126698463

Start: 1066670

End: 1068535

Strand: Direct

Name: amyB [H]

Synonym: CD0886

Alternate gene names: 126698463

Gene position: 1066670-1068535 (Clockwise)

Preceding gene: 126698462

Following gene: 126698464

Centisome position: 24.86

GC content: 27.12

Gene sequence:

>1866_bases
ATGCAAAGTGTAATAGAATATAACTCTTGGGATAAAAATTTTAAGGCTCCGTTTGGAGCTTTAAAATTTGATGAGGAATT
GACCATTTGTGTTAAAGTAAATGAGGGATACAATATCAAGTCTATTTCCCTAGAAATAAATAGAGAAGAAGAAATGAGAA
CAATAACTTTAAATGAAGAATTAGATAATGATAAACTTGGAAAGTGCTTTTGTGGTAAGATTGAAAAGTTTGATGGTACA
GGTGTTTATTTTTATTACTTTAAAGTTGATGTTGAAATGGATGGTCAAATAAAGACTTTATTTTATGGAAAAAACAGAGA
TAATGGATATTCTTGTGAATATAATTATAGTGATATTAATAAATATCAAATCACTGTTTATAAAGATTTTAAAGTACCAA
CATGGTACAAAGAAGGAATACTTTATCATATATTTGTAGATAGATTCAATAATGGAAATCGTAGTGGTAAGGTTGATAAT
CCTAAAAAAAATAGTTTCATATATGGAAATTGGGAAGATACACCTATGTACATAAAGGATAGTCAAGGGGATGTTATAAG
ATGGGATTTCCATGGTGGAAATTTAAGAGGGATAATCAATAAACTTGGATATTTAAAAAAATTAGGTGTAAGTATACTAT
ATCTAAGCCCTATATTTGAAGCCTCTAGTAATCATAAGTATGATACAGGTGACTATAAAAAAATTGACCCAATGTTTGGA
GATGAAGATACATTTAAAGAACTTATTGATAAAGCAAAAGAAAAAGGTATATCAATAGTATTAGATGGAGTTTTTAGTCA
TACTGGAGCAGATAGTAAATATTTTAATATGTATGGAAACTATAATAGTTTAGGAGCATATCAATCTAAAGAATCTCCAT
ATTATTCATGGTATATGTTTGAAGAATTTCCTCAAAAATATAAAAGTTGGTGGGATGTTAAGACATTGCCAAATATAAAT
GAATTAGAACATAGCTATATGGATTATATAATATATGACAATGATAGCGTTATAAATAAATGGGTAAATATGGGTATTAA
GGGCTGGAGACTAGATGTTGCAGACGAATTACCAACTAAGTTTATAAGAGAGTTAAAAAAGGAATTAAAAAAGGCAGATG
ATGACTCAATTCTTATAGGTGAAGTATGGGAAGATGCATCAAATAAGATTAGCTATGGTCAGAGAAGAAGTTATTTATTA
GGTGAAGAATTAGATTCTGTTATGGGTTATCCTTTTAGAAATAACATGTTTTCTTTCTTAAAAGGAGAAATTAATTCATA
TGAACTTTGTAATAGATATATGCAGATTAAAGAAAATTATCCAAAAGAATCATTTAGGTCTAATTTAAATTTAATTGGAA
CACATGATGTTACAAGAGCTAAAACGGAATTAAATGATGATGTAGATTTAGTGAAGCTTGCTGTAGCTATACAGATGACA
TTTGAAGGAGTACCATATATATATTATGGAGATGAAGCAGGTCTTTGTGGAGGTGTTGACCCAGATAATAGAAGAACTTA
TCCATGGAAAAATGAAGACGAAGATATGCTTAATTTTTATAGAGATGTTATAAAAATTAGAAATAAGAATAAACTTTTAA
GTTCTGGAAATACAGAATTTATATATACTAATAATGATTCTGTATTTTCTTTTATTAGAGTTAATGAAAATAATGATAGA
ATGTTAATTTTAGTAAATAGAAGTGAAAATGTGGAAAGTATATCATTATGTATAGAGAGTAGTTTTATTGAGGAAATACC
TATAAAGTATAGTCTTAAAAATGCAAATAGTACTATACAAATTGAAAATAATGAGTTGAAAGTAGATTTAGACTCTAAAT
CATTTAGAATTTTTAGAGTAAATTAA

Upstream 100 bases:

>100_bases
AAAATGTCTTTAGTTAATATAGCTAACTCTGGATTTTTTTCTTCTGATAGAACAATTTCTGAGTATGCAAAAGACATTTG
GTTTAAAAGGGTGTGATTAA

Downstream 100 bases:

>100_bases
ATATCTTTTTTTAGAAAAATAAATAGAAAATAAGGTGAATGTGGAAAGAGTTTTGTTTTATCAAAACTTTTTCTACTTTT
TTTGTATAAATTATCATAAG

Product: alpha-amylase

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Pullulanase type II; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISLEINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGT
GVYFYYFKVDVEMDGQIKTLFYGKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPIFEASSNHKYDTGDYKKIDPMFG
DEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFNMYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNIN
ELEHSYMDYIIYDNDSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISYGQRRSYLL
GEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLNLIGTHDVTRAKTELNDDVDLVKLAVAIQMT
FEGVPYIYYGDEAGLCGGVDPDNRRTYPWKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDR
MLILVNRSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN

Sequences:

>Translated_621_residues
MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISLEINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGT
GVYFYYFKVDVEMDGQIKTLFYGKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPIFEASSNHKYDTGDYKKIDPMFG
DEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFNMYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNIN
ELEHSYMDYIIYDNDSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISYGQRRSYLL
GEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLNLIGTHDVTRAKTELNDDVDLVKLAVAIQMT
FEGVPYIYYGDEAGLCGGVDPDNRRTYPWKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDR
MLILVNRSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN
>Mature_621_residues
MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISLEINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGT
GVYFYYFKVDVEMDGQIKTLFYGKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPIFEASSNHKYDTGDYKKIDPMFG
DEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFNMYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNIN
ELEHSYMDYIIYDNDSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISYGQRRSYLL
GEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLNLIGTHDVTRAKTELNDDVDLVKLAVAIQMT
FEGVPYIYYGDEAGLCGGVDPDNRRTYPWKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDR
MLILVNRSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted, cell wall. Note=It C-terminus may serve as an S-layer anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=387, Percent_Identity=21.7054263565891, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1786604, Length=569, Percent_Identity=29.8769771528998, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1790687, Length=417, Percent_Identity=24.4604316546763, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789995, Length=245, Percent_Identity=29.3877551020408, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321731, Length=481, Percent_Identity=23.2848232848233, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319776, Length=481, Percent_Identity=23.2848232848233, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322241, Length=245, Percent_Identity=23.6734693877551, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=245, Percent_Identity=23.6734693877551, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324416, Length=245, Percent_Identity=23.6734693877551, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=177, Percent_Identity=25.4237288135593, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=253, Percent_Identity=22.9249011857708, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24586593, Length=515, Percent_Identity=22.9126213592233, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583749, Length=203, Percent_Identity=30.0492610837438, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583747, Length=203, Percent_Identity=30.0492610837438, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586599, Length=436, Percent_Identity=23.6238532110092, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586589, Length=102, Percent_Identity=41.1764705882353, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586587, Length=112, Percent_Identity=38.3928571428571, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586591, Length=394, Percent_Identity=23.0964467005076, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221330053, Length=201, Percent_Identity=30.3482587064677, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24586597, Length=382, Percent_Identity=23.8219895287958, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549022, Length=203, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583745, Length=199, Percent_Identity=27.6381909547739, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR001119 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00395 SLH [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 72684; Mature: 72684

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISLEINREEEMRTITLNEE
CCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCEEEEEEEECCCCCEEEEEECCC
LDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFYGKNRDNGYSCEYNYSDIN
CCCCCCCCEEECCCCCCCCCEEEEEEEEEEEEECCEEEEEEEECCCCCCEEEEECCCCCC
KYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDNPKKNSFIYGNWEDTPMYIKD
EEEEEEEECCCCCCCHHCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEEEEC
SQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPIFEASSNHKYDTGDYKKIDPMFG
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCCCCCCCCEEECCCCC
DEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFNMYGNYNSLGAYQSKESPYYSWYMF
CHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEH
EEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDNDSVINKWVNMGIKGWRLDVADELPTK
HHHHHHHHCCCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHHHCCCCEEEEECHHHHHHH
FIRELKKELKKADDDSILIGEVWEDASNKISYGQRRSYLLGEELDSVMGYPFRNNMFSFL
HHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
KGEINSYELCNRYMQIKENYPKESFRSNLNLIGTHDVTRAKTELNDDVDLVKLAVAIQMT
HCCCCHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCCHHHHHHCCCCHHHHEEEEEEEEE
FEGVPYIYYGDEAGLCGGVDPDNRRTYPWKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEF
ECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
IYTNNDSVFSFIRVNENNDRMLILVNRSENVESISLCIESSFIEEIPIKYSLKNANSTIQ
EEECCCCEEEEEEEECCCCEEEEEEECCCCCCEEEEEHHHHHHHHCCCEEEEECCCCEEE
IENNELKVDLDSKSFRIFRVN
EECCEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISLEINREEEMRTITLNEE
CCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCEEEEEEEECCCCCEEEEEECCC
LDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFYGKNRDNGYSCEYNYSDIN
CCCCCCCCEEECCCCCCCCCEEEEEEEEEEEEECCEEEEEEEECCCCCCEEEEECCCCCC
KYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDNPKKNSFIYGNWEDTPMYIKD
EEEEEEEECCCCCCCHHCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEEEEC
SQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPIFEASSNHKYDTGDYKKIDPMFG
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCCCCCCCCEEECCCCC
DEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFNMYGNYNSLGAYQSKESPYYSWYMF
CHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEH
EEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDNDSVINKWVNMGIKGWRLDVADELPTK
HHHHHHHHCCCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHHHCCCCEEEEECHHHHHHH
FIRELKKELKKADDDSILIGEVWEDASNKISYGQRRSYLLGEELDSVMGYPFRNNMFSFL
HHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
KGEINSYELCNRYMQIKENYPKESFRSNLNLIGTHDVTRAKTELNDDVDLVKLAVAIQMT
HCCCCHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCCHHHHHHCCCCHHHHEEEEEEEEE
FEGVPYIYYGDEAGLCGGVDPDNRRTYPWKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEF
ECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
IYTNNDSVFSFIRVNENNDRMLILVNRSENVESISLCIESSFIEEIPIKYSLKNANSTIQ
EEECCCCEEEEEEEECCCCEEEEEEECCCCCCEEEEEHHHHHHHHCCCEEEEECCCCEEE
IENNELKVDLDSKSFRIFRVN
EECCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8195085 [H]