BacMap

Definition	Clostridium difficile 630 chromosome, complete genome.
Accession	NC_009089
Length	4,290,252

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The map label for this gene is crt1 [H]

Identifier: 126698380

GI number: 126698380

Start: 972321

End: 973103

Strand: Direct

Name: crt1 [H]

Synonym: CD0800

Alternate gene names: 126698380

Gene position: 972321-973103 (Clockwise)

Preceding gene: 126698379

Following gene: 126698381

Centisome position: 22.66

GC content: 33.33

Gene sequence:

>783_bases
ATGGAAAATTTGAACAATCTTAAGGTGGAGGTTAAGGACAAAGTCTGTGTTATTACTATAAATAGACCTAAAGCTTTAAA
TGCTCTTAATAGTGACACTCTTAGGGAACTATCACAAGTTATTGATGTAGTATCAGAAAATGAGGCAATATTAGGAGTTA
TTATTACTGGAGAAGGTAAAGTTTTTGTAGCCGGTGCTGATATACGCCAGATGCAAAACTATAAAAGTGAGGAAGGCAGA
AAATATGCAGGTTATGCTCAGGGAATTTTTGATAAAATTGAAGCTCTTGAGAAAACAGTAATAGCAGCAGTTAATGGATA
TGCATTAGGTGGTGGATGTGAACTTGCTATGAGTTGTGATATTAGAATTGCATCAGAAAAGGCTATATTTGGACAACCTG
AAGTTAATTTAGGAGTTATTCCATGTTTTGGTGGAACTCAAAGATTATCAAGACTTGTTGGAACTGGAATAGCTAAAGAG
TTAATTTTTACAGGGAGACAAGTAAATGCAGAAGAAGCTAAATCTATTGGGCTTATTAATAAAGTGGTTCCAAGTGATTT
ACTTTTAGAAGAGTCTATGAAGATGATGAACCAAATAGTTGAAAAAGCGCCAATAGCAATAAGATATGCAAAAGTTGTTA
TTAACAAAGGGATAGATATGGATTTAAAGAATGCTCTTGAACTAGAGAAAGATATTGCAGGTTTAACTTTTGCTACAAGA
GATAAGCAAGAAGGAATGAATGCATTTATAGAAAAGAGAAAGCCAGTTTTTGAAAATAAGTAG

Upstream 100 bases:

>100_bases
GAACTTCAATGGTGATTGGCATAGTACTTGCTATTTTATATTAACAAGAAATGTAGAAAAAATAACAATGTATAATGGGG
GGAATATGATGGAATCTGTA

Downstream 100 bases:

>100_bases
CTATTATAACTAAAATTGGAACAATGTAGAATATATTCAAAATCTAAATTTCGTGAAAAAATACATATAGTTTAATTTTA
AAAGGAGTTAATTTATGAAT

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGR
KYAGYAQGIFDKIEALEKTVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKE
LIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGIDMDLKNALELEKDIAGLTFATR
DKQEGMNAFIEKRKPVFENK

Sequences:

>Translated_260_residues
MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGR
KYAGYAQGIFDKIEALEKTVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKE
LIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGIDMDLKNALELEKDIAGLTFATR
DKQEGMNAFIEKRKPVFENK
>Mature_260_residues
MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGR
KYAGYAQGIFDKIEALEKTVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKE
LIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGIDMDLKNALELEKDIAGLTFATR
DKQEGMNAFIEKRKPVFENK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=44.3548387096774, Blast_Score=216, Evalue=1e-56,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=35.8565737051793, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=34.412955465587, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI20127408, Length=220, Percent_Identity=35.9090909090909, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=30.6451612903226, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=29.5880149812734, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI68989263, Length=214, Percent_Identity=34.5794392523364, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=27.0742358078603, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=27.0742358078603, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI221136756, Length=231, Percent_Identity=25.1082251082251, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI221136753, Length=231, Percent_Identity=25.1082251082251, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI221307494, Length=231, Percent_Identity=25.1082251082251, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157694520, Length=209, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI25453481, Length=241, Percent_Identity=22.8215767634855, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI51538573, Length=241, Percent_Identity=22.8215767634855, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4757968, Length=241, Percent_Identity=22.8215767634855, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI49355787, Length=241, Percent_Identity=22.8215767634855, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4757966, Length=220, Percent_Identity=23.6363636363636, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI51538581, Length=220, Percent_Identity=23.6363636363636, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI116235487, Length=192, Percent_Identity=27.0833333333333, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594471, Length=250, Percent_Identity=29.6, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI37594469, Length=250, Percent_Identity=29.6, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=26.6355140186916, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=26.6355140186916, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=26.2672811059908, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI261878539, Length=114, Percent_Identity=40.3508771929825, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI62530384, Length=248, Percent_Identity=24.5967741935484, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=36.5758754863813, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=33.9622641509434, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=33.587786259542, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=38.3084577114428, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=32.6424870466321, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082183, Length=249, Percent_Identity=34.9397590361446, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=31.3725490196078, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=43.8735177865613, Blast_Score=226, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=42.0408163265306, Blast_Score=197, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=35.9447004608295, Blast_Score=150, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=31.7829457364341, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17534483, Length=238, Percent_Identity=29.8319327731092, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=29.4605809128631, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=33.1491712707182, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=221, Percent_Identity=34.3891402714932, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=221, Percent_Identity=34.3891402714932, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=221, Percent_Identity=33.9366515837104, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=190, Percent_Identity=32.6315789473684, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144157, Length=190, Percent_Identity=32.6315789473684, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=240, Percent_Identity=27.5, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=262, Percent_Identity=26.3358778625954, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=25.1256281407035, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=26.1603375527426, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129971, Length=265, Percent_Identity=44.1509433962264, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24653477, Length=265, Percent_Identity=44.1509433962264, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=37.007874015748, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19922422, Length=243, Percent_Identity=28.3950617283951, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19920382, Length=208, Percent_Identity=32.2115384615385, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19921000, Length=183, Percent_Identity=39.344262295082, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583077, Length=183, Percent_Identity=39.344262295082, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583079, Length=183, Percent_Identity=39.344262295082, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21357171, Length=198, Percent_Identity=30.8080808080808, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24650670, Length=199, Percent_Identity=29.1457286432161, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24654903, Length=226, Percent_Identity=26.1061946902655, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583165, Length=180, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921018, Length=179, Percent_Identity=28.4916201117318, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571729, Length=140, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571730, Length=140, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28421; Mature: 28421

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVVSENEAILGVIITGEGK
CCCCCCCEEEEECCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCE
VFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEKTVIAAVNGYALGGGCELAMSCD
EEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEEC
IRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKELIFTGRQVNAEEAKSIGLIN
EEEECCCEECCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHH
KVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGIDMDLKNALELEKDIAGLTFATR
HHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEECC
DKQEGMNAFIEKRKPVFENK
CHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQVIDVVSENEAILGVIITGEGK
CCCCCCCEEEEECCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCE
VFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEKTVIAAVNGYALGGGCELAMSCD
EEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEEC
IRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTGIAKELIFTGRQVNAEEAKSIGLIN
EEEECCCEECCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHH
KVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVINKGIDMDLKNALELEKDIAGLTFATR
HHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEECC
DKQEGMNAFIEKRKPVFENK
CHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]