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The map label for this gene is lpdA [H]

Identifier: 126698303

GI number: 126698303

Start: 884594

End: 885979

Strand: Direct

Name: lpdA [H]

Synonym: CD0723

Alternate gene names: 126698303

Gene position: 884594-885979 (Clockwise)

Preceding gene: 126698302

Following gene: 126698304

Centisome position: 20.62

GC content: 34.92

Gene sequence:

>1386_bases
ATGAAGATAGTAGTAGTAGGTGGTGGACCAGGAGGATATGTAGCAGCTATAAAAGCTTCTATGCTTGGTGCAGATGTAAC
AGTTGTTGAAAAAAGAAGAGTTGGAGGAACTTGCTTAAATGCAGGATGTATACCAACTAAAGCACTTCTTGCTTCTTCAG
GAGTATTGAATACTGTAAAAGAAGCAAAGGATTTTGGTATAGAAATAGATGGTACAGTTAAGCCAAACTTCACTGCCATA
ATGGAAAGAAAAAATAAAGTTGTAAACCAATTAATAAGTGGTATAGAGTTCTTATTTGAAAAAAGAGGAGTAAACTTAGT
TAATGGTTTTGGAAAATTAATTGATAAAAACACTATAGAAGTAACTAAAGATGATGGAACAGTAGAAACTATAAAAGCAG
ATAAGATAATACTAGCAAATGGTTCTGTTCCAGTTGTACCAAGAATGTTCCCATATGATGGAAAAGTTGTAATAACTAGT
GATGAAGTTCTTGGACTTGAAGAGATACCTGAGTCTATGTTAATAGTAGGTGGTGGAGTTATAGGATGTGAGATAGGGCA
GTTCTTTAGAGCCTTAGGAACAGAAGTAACTATAGTTGAGATGGTTGACCAAATACTATTAAATGAAGACAAAGATGTAG
CTAAACAACTACTTAGACAATTTAAGAAAGATAAAATTAAGGTTATTACAGGTATTGGAGTACAAACTTGTGAAGTAGTT
GATGGTAAGGCTGTTGCAACTCTTTCTAATGGAAAGGTTATAGAAGCACAATATGCATTAGTATGTGTTGGTAGAAGACC
TAACCTTGACAATTCTGGAGTAGAAGATATAGGAATTGAAATGGAAAGAGGAAAAGTAGTAGTTAATGAACATCTTGAAA
CTAATGTAGAAGGTATATACGCAATAGGCGATATAATAGATACTCCTTTCCTAGCACATGTTGCTTCTAAAGAAGGTATC
GTAGCAGTTGAAAATGCTTTAGGTAAGACTAAGGTAGTAGATTATAGAGCAATTCCAAGATGTGTTTATACTGAACCTGA
AGTTGCTGGTGTTGGTAAGACTGAAAAGCAACTTGAAGCTGAAGGTGTAGAATACAATGTAGGTCAATTTGATTTTAGAG
GTCTTGGAAAAGCACAAGCTATAGGACATTTCCAAGGATTTGTAAAAGTTATAGCTGACAAAGAAACTGATAAGATAATA
GGTGCAGCAGTAGTAGGACCTCATGCTACAGATTTATTGACAGAGTTGTCACTTGCTGTTCATCTAGGATTAACAGTAGA
ACAAGTAGGGGATGCAATACATCCACATCCGAGTTTATCTGAAGGGCTAATGGAAGCACTTCATGATGTACATGGAGAAT
GTGTTCATTCTGTACCTAAATTATAA

Upstream 100 bases:

>100_bases
ATGGCAATTGGGGCAGAAGAGAATATACCTGCTATACTGGATGCCGCAGGTTTATAGTATTGCAAGATTGTAGATTATAT
TAGGTTTGGGGGAATTAAAG

Downstream 100 bases:

>100_bases
TAGACAGACAGAGTACATAAGGCTCTGTGGCTAGATTTAAGGGGGAAGTCTTAAAATGGGATACAATATAGCTGTTGCAG
GAAAAGGTGGAACTGGTAAA

Product: carbon monoxide dehydrogenase/acetyl-CoA synthase complex, dihydrolipoyl dehydrogenase subunit

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCIPTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAI
MERKNKVVNQLISGIEFLFEKRGVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLLRQFKKDKIKVITGIGVQTCEVV
DGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVEDIGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGI
VAVENALGKTKVVDYRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIADKETDKII
GAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDVHGECVHSVPKL

Sequences:

>Translated_461_residues
MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCIPTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAI
MERKNKVVNQLISGIEFLFEKRGVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLLRQFKKDKIKVITGIGVQTCEVV
DGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVEDIGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGI
VAVENALGKTKVVDYRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIADKETDKII
GAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDVHGECVHSVPKL
>Mature_461_residues
MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCIPTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAI
MERKNKVVNQLISGIEFLFEKRGVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLLRQFKKDKIKVITGIGVQTCEVV
DGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVEDIGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGI
VAVENALGKTKVVDYRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIADKETDKII
GAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDVHGECVHSVPKL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=41.0869565217391, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=26.9315673289183, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=26.9953051643192, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=26.9953051643192, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=26.9953051643192, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=26.9953051643192, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=26.9953051643192, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=26.7391304347826, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI22035672, Length=456, Percent_Identity=25, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=25.2212389380531, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=38.3928571428571, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI87082354, Length=416, Percent_Identity=31.4903846153846, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=26.9911504424779, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=25.6944444444444, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=42.0131291028446, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=27.2151898734177, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=436, Percent_Identity=24.5412844036697, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=435, Percent_Identity=23.6781609195402, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=435, Percent_Identity=23.6781609195402, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=39.1120507399577, Blast_Score=296, Evalue=6e-81,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=29.7872340425532, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=446, Percent_Identity=25.7847533632287, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.9190371991247, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24640549, Length=463, Percent_Identity=28.2937365010799, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24640553, Length=463, Percent_Identity=28.2937365010799, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640551, Length=463, Percent_Identity=28.2937365010799, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17737741, Length=476, Percent_Identity=26.890756302521, Blast_Score=132, Evalue=6e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49136; Mature: 49136

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCIPTKALLASSGVLNTVK
CEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHCCCCEEECCCCCCHHHHHHCCCHHHHHH
EAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKRGVNLVNGFGKLIDKNTIE
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
VTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITSDEVLGLEEIPESMLIVGGGV
EECCCCCEEEEECCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCEEEECCCE
IGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLLRQFKKDKIKVITGIGVQTCEVV
EEHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEE
DGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVEDIGIEMERGKVVVNEHLETNVEGIY
CCCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCHHCCEEEECCEEEEECCHHCCCCEEE
AIGDIIDTPFLAHVASKEGIVAVENALGKTKVVDYRAIPRCVYTEPEVAGVGKTEKQLEA
EEHHHHCCCHHHHHCCCCCEEEECCCCCCCEEEEHHCCCCEECCCCCCCCCCCCHHHHHH
EGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIADKETDKIIGAAVVGPHATDLLTELSLAV
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HLGLTVEQVGDAIHPHPSLSEGLMEALHDVHGECVHSVPKL
HCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCIPTKALLASSGVLNTVK
CEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHCCCCEEECCCCCCHHHHHHCCCHHHHHH
EAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKRGVNLVNGFGKLIDKNTIE
HHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEE
VTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITSDEVLGLEEIPESMLIVGGGV
EECCCCCEEEEECCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCEEEECCCE
IGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLLRQFKKDKIKVITGIGVQTCEVV
EEHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEE
DGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVEDIGIEMERGKVVVNEHLETNVEGIY
CCCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCHHCCEEEECCEEEEECCHHCCCCEEE
AIGDIIDTPFLAHVASKEGIVAVENALGKTKVVDYRAIPRCVYTEPEVAGVGKTEKQLEA
EEHHHHCCCHHHHHCCCCCEEEECCCCCCCEEEEHHCCCCEECCCCCCCCCCCCHHHHHH
EGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIADKETDKIIGAAVVGPHATDLLTELSLAV
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHH
HLGLTVEQVGDAIHPHPSLSEGLMEALHDVHGECVHSVPKL
HCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]